scholarly journals Mechanism of Action Studies of Lomaiviticin A and the Monomeric Lomaiviticin Aglycon. Selective and Potent Activity Toward DNA Double-Strand Break Repair-Deficient Cell Lines

2015 ◽  
Vol 137 (17) ◽  
pp. 5741-5747 ◽  
Author(s):  
Laureen C. Colis ◽  
Denise C. Hegan ◽  
Miho Kaneko ◽  
Peter M. Glazer ◽  
Seth B. Herzon
Keyword(s):  
Cell Lines ◽  
Mechanism Of Action ◽  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Deficient Cell ◽  
Dna Double Strand Break ◽  
Break Repair

scholarly journals Fidelity of DNA double-strand break repair in heterozygous cell lines harbouring BRCA1 missense mutations

Oncogene ◽  
2003 ◽  
Vol 23 (4) ◽  
pp. 914-919 ◽  
Author(s):  
Isabelle Coupier ◽  
Céline Baldeyron ◽  
Alexandra Rousseau ◽  
Véronique Mosseri ◽  
Sabine Pages-Berhouet ◽  
...  
Keyword(s):  
Cell Lines ◽  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Missense Mutations ◽  
Dna Double Strand Break ◽  
Break Repair

scholarly journals Assessing Candidate Gene nsSNPs for Phenotypic Differences in Double-Strand Break Repair Using Radiation-InducedγH2A.X Foci

2008 ◽  
Vol 2008 ◽  
pp. 1-8 ◽  
Author(s):  
Christina A. Markunas ◽  
David M. Umbach ◽  
Zongli Xu ◽  
Jack A. Taylor
Keyword(s):  
Cell Lines ◽  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Screening Criteria ◽  
Phenotypic Differences ◽  
Dna Double Strand Break ◽  
Significant Difference ◽  
Break Repair ◽  
Repair Genes

Nonsynonymous SNPs (nsSNPs) in DNA repair genes may be important determinants of DNA damage and cancer risk. We applied a set of screening criteria to a large number of nsSNPs and selected a subset of SNPs that were likely candidates for phenotypic effects on DNA double-strand break repair (DSBR). In order to induce and follow DSBR, we exposed panels of cell lines to gamma irradiation and followed the formation and disappearance ofγH2A.X foci over time. All panels of cell lines showed significant increases in number, intensity, and area of foci at both the 1-hour and 3-hour time points. Twenty four hours following exposure, the number of foci returned to preexposure levels in all cell lines, whereas the size and intensity of foci remained significantly elevated. We saw no significant difference inγH2A.X foci between controls and any of the panels of cell lines representing the different nsSNPs.

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