Molecularly Imprinted Synthetic Glucosidase for the Hydrolysis of Cellulose in Aqueous and Nonaqueous Solutions

2021 ◽  
Author(s):  
Xiaowei Li ◽  
Milad Zangiabadi ◽  
Yan Zhao
Keyword(s):  
Nonaqueous Solutions ◽  
Molecularly Imprinted ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

Immobilized Nanoparticles-Mediated Enzymatic Hydrolysis of Cellulose for Clean Sugar Production: A Novel Approach

2019 ◽  
Vol 15 (3) ◽  
pp. 296-303 ◽  
Author(s):  
Swapnil Gaikwad ◽  
Avinash P. Ingle ◽  
Silvio Silverio da Silva ◽  
Mahendra Rai
Keyword(s):  
Catalytic Activity ◽  
Enzymatic Hydrolysis ◽  
Immobilized Enzyme ◽  
Free Enzyme ◽  
Magnetic Nanomaterials ◽  
Sugar Production ◽  
Cellulase Enzyme ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

Background: Enzymatic hydrolysis of cellulose is an expensive approach due to the high cost of an enzyme involved in the process. The goal of the current study was to apply magnetic nanomaterials as a support for immobilization of enzyme, which helps in the repeated use of immobilized enzyme for hydrolysis to make the process cost-effective. In addition, it will also provide stability to enzyme and increase its catalytic activity. Objective: The main aim of the present study is to immobilize cellulase enzyme on Magnetic Nanoparticles (MNPs) in order to enable the enzyme to be re-used for clean sugar production from cellulose. Methods: MNPs were synthesized using chemical precipitation methods and characterized by different techniques. Further, cellulase enzyme was immobilized on MNPs and efficacy of free and immobilized cellulase for hydrolysis of cellulose was evaluated. Results: Enzymatic hydrolysis of cellulose by immobilized enzyme showed enhanced catalytic activity after 48 hours compared to free enzyme. In first cycle of hydrolysis, immobilized enzyme hydrolyzed the cellulose and produced 19.5 ± 0.15 gm/L of glucose after 48 hours. On the contrary, free enzyme produced only 13.7 ± 0.25 gm/L of glucose in 48 hours. Immobilized enzyme maintained its stability and produced 6.15 ± 0.15 and 3.03 ± 0.25 gm/L of glucose in second and third cycle, respectively after 48 hours. Conclusion: This study will be very useful for sugar production because of enzyme binding efficiency and admirable reusability of immobilized enzyme, which leads to the significant increase in production of sugar from cellulosic materials.

In Situ Microscopy for In-line Monitoring of the Enzymatic Hydrolysis of Cellulose

2013 ◽  
Vol 85 (17) ◽  
pp. 8121-8126 ◽  
Author(s):  
Britta Opitz ◽  
Andreas Prediger ◽  
Christian Lüder ◽  
Marrit Eckstein ◽  
Lutz Hilterhaus ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

Enhanced Hydrolysis of Cellulose to Reducing Sugars on Kaolinte Clay Activated by Mineral Acid

2021 ◽  
Author(s):  
Yuxiao Dong ◽  
Dongshen Tong ◽  
Laibin Ren ◽  
Xingtao Chen ◽  
Hao Zhang ◽  
...  
Keyword(s):  
Reducing Sugars ◽  
Mineral Acid ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

Enhanced ammonia adsorption and separation by a molecularly imprinted polymer after acid hydrolysis of its ester crosslinker

2021 ◽  
Vol 412 ◽  
pp. 125145
Author(s):  
Zhangliang Han ◽  
Yangjie Xu ◽  
Haozhong Tian ◽  
Jiahao Liang ◽  
Dezhi Sun
Keyword(s):  
Acid Hydrolysis ◽  
Molecularly Imprinted Polymer ◽  
Imprinted Polymer ◽  
Ammonia Adsorption ◽  
Molecularly Imprinted ◽  
Hydrolysis Of

Role and significance of beta-glucosidases in the hydrolysis of cellulose for bioethanol production

2013 ◽  
Vol 127 ◽  
pp. 500-507 ◽  
Author(s):  
Reeta Rani Singhania ◽  
Anil Kumar Patel ◽  
Rajeev K. Sukumaran ◽  
Christian Larroche ◽  
Ashok Pandey
Keyword(s):  
Bioethanol Production ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

Enzymatic hydrolysis of cellulose in aqueous two-phase systems. II. Semicontinuous conversion of a model substrate, solka floc BW 200

1985 ◽  
Vol 27 (7) ◽  
pp. 1044-1050 ◽  
Author(s):  
Folke Tjerneld ◽  
Ingrid Persson ◽  
Per-Åke Albertsson ◽  
Bärbel Hahn-Hägerdal
Keyword(s):  
Enzymatic Hydrolysis ◽  
Two Phase ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Of Cellulose ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Hydrolysis Of

MgO-Fe3O4 linked cellulase enzyme complex improves the hydrolysis of cellulose from Chlorella sp. CYB2

2017 ◽  
Vol 122 ◽  
pp. 22-30 ◽  
Author(s):  
Rajendran Velmurugan ◽  
Aran Incharoensakdi
Keyword(s):  
Enzyme Complex ◽  
Cellulase Enzyme ◽  
Chlorella Sp ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

Observations on the complex interactions involved in the enzymatic hydrolysis of cellulose

1984 ◽  
Vol 9 (4) ◽  
pp. 313-314
Author(s):  
T. M. Wood ◽  
S. I. McCrae ◽  
C. A. Wilson
Keyword(s):  
Enzymatic Hydrolysis ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Complex Interactions ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

The Effect of Dicarboxylic Acid Catalyst Structure on Hydrolysis of Cellulose Model Compound D-Cellobiose in Water

2021 ◽  
Vol 08 ◽  
Author(s):  
Harshica Fernando ◽  
Ananda S. Amarasekara
Keyword(s):  
Dicarboxylic Acid ◽  
Model Compound ◽  
Acid Catalyst ◽  
Catalytic Activities ◽  
Polycarboxylic Acids ◽  
Hydrolysis Of Cellulose ◽  
Phthalic Acids ◽  
Turn Over Frequency ◽  
Turn Over ◽  
Hydrolysis Of

Background: Polycarboxylic acids are of interest as simple mimics for cellulase enzyme catalyzed depolymerization of cellulose. In this study, DFT calculations were used to investigate the effect of structure on dicarboxylic acid organo-catalyzed hydrolysis of cellulose model compound D-cellobiose to D-glucose. Methods: Binding energy of the complex formed between D-cellobiose and acid (Ebind), as well as glycosidic oxygen to dicarboxylic acid closest acidic H distance were studied as key parameters affecting the turn over frequency of hydrolysis in water. Result: α-D-cellobiose - dicarboxylic acid catalyst down face approach showed high Ebind values for five of the six acids studied; indicating the favorability of down face approach. Maleic, cis-1,2-cyclohexane dicarboxylic, and phthalic acids with the highest catalytic activities showed glycosidic oxygen to dicarboxylic acid acidic H distances 3.5-3.6 Å in the preferred configuration. Conclusion: The high catalytic activities of these acids may be due to the rigid structure, where acid groups are held in a fixed geometry.

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