Role and significance of beta-glucosidases in the hydrolysis of cellulose for bioethanol production

The state of art for the bioethanol production from paper fibre residue using diluted alkali hydrolysis and fermentation processes was evaluated. Hydrolysis of paper fibre residue with diluted sodium hydroxide at various time period, temperature and concentration were investigated. The paper fibre residue was pre-steamed, impregnated with diluted NaOH (0 to 25%) and subsequently hydrolyzed in a reactor at temperatures that ranged between 30 to 50oC, for reaction time between 30 minutes to 150 minutes. The highest yield of monosaccharide (indicating the efficient hydrolysis of cellulose and hemi cellulose) was found at a temperature of 35oC for a reaction time of 90 minutes. Fermentability of hemicelluloses hydrolysate was tested using monosaccharide fermenting microorganismPenicillium chrysogenumandSaccharomyces cereviacea. The fermentability of the hydrolysate decreased strongly for hydrolysate produced at temperature higher than 50oC. The ethanol concentration of monosaccharide hydrolysate was found to be 34.06 g/L and the ethanol yield was 0.097 g/g.

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Microwave Assisted Hydrolysis of Cellulose to Release Sugars from Pongamia Oil Cake for its use in Bioethanol Production

Chemical Science Transactions ◽

10.7598/cst2018.1557 ◽

2018 ◽

Vol 7 (4) ◽

Keyword(s):

Bioethanol Production ◽

Microwave Assisted ◽

Hydrolysis Of Cellulose ◽

Pongamia Oil ◽

Hydrolysis Of

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BIOETHANOL PRODUCTION FROM COCONUT HUSK USING THE WET GAMMA IRADIATION METHOD

Jurnal Forum Nuklir ◽

10.17146/jfn.2020.14.2.5908 ◽

2021 ◽

Vol 14 (2) ◽

pp. 43

Author(s):

Putra Oktavianto ◽

Risdiyana Setiawan ◽

Ilhami Ariyanti ◽

Muhammad Fadhil Jamil

Keyword(s):

Saccharomyces Cerevisiae ◽

Cellulose Hydrolysis ◽

Raw Material ◽

Bioethanol Production ◽

Coconut Husk ◽

Hydrolysis Process ◽

Hydrolysis Of Cellulose ◽

Ethanol Ethanol ◽

Hydrolysis Of ◽

Low Dosage

BIOETHANOL PRODUCTION FROM COCONUT HUSK USING the WET GAMMA IRRADIATION METHOD. The use of coconut husk has only been used as a material for making handicrafts such as ropes, brooms, mats, and others or just burned. The combustion of coconut husk can cause air pollution. In fact, coconut husk can be used as a raw material for bioethanol production so that the beneficial value of coconut husk will also increase. One way of bioethanol production from coconut husk is by irradiating the coconut husk. The coconut husk irradiation technique to be carried out in this study is the wet irradiation technique. Wet irradiation is carried out to accelerate the process of bioethanol production because at the time of irradiation, cellulose has been hydrolyzed and glucose has been formed so that it is more efficient in time and use of the material so that the cellulose hydrolysis process is not necessary. The coconut husk samples were wet because they were mixed with 4% NaOH and were irradiated using a gamma irradiator from STTN-BATAN Yogyakarta with a dose of 30 kGy and 50 kGy and 0 kGy (or without irradiation). Then the sample is fermented with the fungus Saccharomyces Cerevisiae from tape yeast to form ethanol. Ethanol is purified and then analyzed for concentrations using pycnometric and refractometric methods. The result is that the highest ethanol content is without irradiation (0 kGy), this is due to the low dosage used. However, the main point in this wet method research is evidence of hydrolysis of cellulose by the formation of gluoxane after irradiated wet coconut husk, and with Fehling A and B analysis, brown deposits are seen proving that glucose has been formed.

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Simultaneous Sachharification and Fermentation of Rice Residues and its Comparative Analysis for Bioethanol Production

Defence Life Science Journal ◽

10.14429/dlsj.4.14188 ◽

2019 ◽

Vol 4 (3) ◽

pp. 158-162

Author(s):

G Sinha ◽

S Tiwari ◽

S K Jadhav

Keyword(s):

Plant Biomass ◽

World Population ◽

Bioethanol Production ◽

Biological Pretreatment ◽

Fermentation Efficiency ◽

Hydrolysis Of Cellulose ◽

Feedstock Availability ◽

Hydrolysis Of ◽

Rice Residues ◽

Selection Of

Energy consumption has inflated steadily over the last century because the world population has fully grown and additional countries became industrialised. Bioethanol is an alcohol produced by fermentation of plant biomass, containing carbohydrate and its production depends upon feedstock availability, variability, and sustainability. The selection of feedstock and its pretreatment is an important part of bioethanol production process. In present work, the exploration of the potential of agro-waste rice residues such as, rice bran and rice husk was done, because it contains sufficient amount of carbohydrate which can be ferment into bioethanol. The aim of the research was also to investigate how different pretreatment methods with moderate conditions differ in hydrolysis and fermentation efficiencies. Pretreatment plays an important role in the hydrolysis of cellulose and lignocellulose. It was found that biological pretreatment was a most effective method in terms of production of bioethanol and it enhances the production as well as fermentation efficiency.

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The effect of the implementation of ultrasound in enzyme separation

Analecta Technica Szegedinensia ◽

10.14232/analecta.2015.2.34-41 ◽

2015 ◽

Vol 9 (2) ◽

pp. 34-41

Author(s):

Kübra Keçeci ◽

Balázs Lemmer ◽

Szabolcs Kertész ◽

Gábor Keszthelyi-Szabó ◽

Zsuzsanna László ◽

...

Keyword(s):

Enzymatic Degradation ◽

Potential Method ◽

Crystalline Cellulose ◽

Bioethanol Production ◽

Important Research ◽

Permeate Flux ◽

Research Issues ◽

Enzyme Recycling ◽

Hydrolysis Of Cellulose ◽

Hydrolysis Of

Enzymes are biological catalysts that generally are designed to do one job well, but to do one job only. Therefore, the enzymes that catalyze the hydrolysis of cellulose to sugar do not break down the sugars. Enzymatic hydrolysis processes have been under development for only 10 years. The important research issues include understanding the processes necessary to render the crystalline cellulose easily digestible, understanding and improving the basic mechanisms in the hydrolysis step, and developing better and less expensive enzymes. The other way to make a process less expensive may be the recycling of enzymes. The essential unit operation in the bioethanol production is the cellulose enzymatic degradation, so the question of recycling is very important. In our work the sonication assisted ultrafiltration was investigated as a potential method for enzyme recycling. The results showed the ultrasound effects the permeate flux since the resistance is reduced by the sonication. The sonicated enzyme keeps its activity so the recycling mechanism might be used for bioethanol production.

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Immobilized Nanoparticles-Mediated Enzymatic Hydrolysis of Cellulose for Clean Sugar Production: A Novel Approach

Current Nanoscience ◽

10.2174/1573413714666180611081759 ◽

2019 ◽

Vol 15 (3) ◽

pp. 296-303 ◽

Cited By ~ 5

Author(s):

Swapnil Gaikwad ◽

Avinash P. Ingle ◽

Silvio Silverio da Silva ◽

Mahendra Rai

Keyword(s):

Catalytic Activity ◽

Enzymatic Hydrolysis ◽

Immobilized Enzyme ◽

Free Enzyme ◽

Magnetic Nanomaterials ◽

Sugar Production ◽

Cellulase Enzyme ◽

Enzymatic Hydrolysis Of Cellulose ◽

Hydrolysis Of Cellulose ◽

Hydrolysis Of

Background: Enzymatic hydrolysis of cellulose is an expensive approach due to the high cost of an enzyme involved in the process. The goal of the current study was to apply magnetic nanomaterials as a support for immobilization of enzyme, which helps in the repeated use of immobilized enzyme for hydrolysis to make the process cost-effective. In addition, it will also provide stability to enzyme and increase its catalytic activity. Objective: The main aim of the present study is to immobilize cellulase enzyme on Magnetic Nanoparticles (MNPs) in order to enable the enzyme to be re-used for clean sugar production from cellulose. Methods: MNPs were synthesized using chemical precipitation methods and characterized by different techniques. Further, cellulase enzyme was immobilized on MNPs and efficacy of free and immobilized cellulase for hydrolysis of cellulose was evaluated. Results: Enzymatic hydrolysis of cellulose by immobilized enzyme showed enhanced catalytic activity after 48 hours compared to free enzyme. In first cycle of hydrolysis, immobilized enzyme hydrolyzed the cellulose and produced 19.5 ± 0.15 gm/L of glucose after 48 hours. On the contrary, free enzyme produced only 13.7 ± 0.25 gm/L of glucose in 48 hours. Immobilized enzyme maintained its stability and produced 6.15 ± 0.15 and 3.03 ± 0.25 gm/L of glucose in second and third cycle, respectively after 48 hours. Conclusion: This study will be very useful for sugar production because of enzyme binding efficiency and admirable reusability of immobilized enzyme, which leads to the significant increase in production of sugar from cellulosic materials.

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