Membrane Mimetic Chemistry in Artificial Cells

2021 ◽  
Author(s):  
Jacob A. Vance ◽  
Neal K. Devaraj
Keyword(s):  
Artificial Cells ◽  
Membrane Mimetic

Artificial cells containing sustainable energy conversion engines

2019 ◽  
Vol 3 (5) ◽  
pp. 573-578 ◽  
Author(s):  
Kwanwoo Shin
Keyword(s):  
Energy Conversion ◽  
Nicotinamide Adenine Dinucleotide ◽  
Sustainable Energy ◽  
Living Cells ◽  
Energy Transduction ◽  
Artificial Cells ◽  
Energy Conversion Process ◽  
Metabolic Reactions ◽  
Metabolic Activities ◽  
Reduced Nicotinamide Adenine Dinucleotide

Living cells naturally maintain a variety of metabolic reactions via energy conversion mechanisms that are coupled to proton transfer across cell membranes, thereby producing energy-rich compounds. Until now, researchers have been unable to maintain continuous biochemical reactions in artificially engineered cells, mainly due to the lack of mechanisms that generate energy-rich resources, such as adenosine triphosphate (ATP) and reduced nicotinamide adenine dinucleotide (NADH). If these metabolic activities in artificial cells are to be sustained, reliable energy transduction strategies must be realized. In this perspective, this article discusses the development of an artificially engineered cell containing a sustainable energy conversion process.

Membrane mimetic chemistry

AccessScience ◽  
2015 ◽  
Keyword(s):  
Membrane Mimetic

Artificial cells, cell engineering and therapy

2007 ◽  
Author(s):  
S. Prakash
Keyword(s):  
Cell Engineering ◽  
Artificial Cells

A Cascade Signaling Network between Artificial Cells Switching Activity of Synthetic Transmembrane Channels

2020 ◽  
Author(s):  
Qiuxia Yang ◽  
Zhenzhen Guo ◽  
Hui Liu ◽  
Ruizi Peng ◽  
Liujun Xu ◽  
...  
Keyword(s):  
Signaling Network ◽  
Switching Activity ◽  
Artificial Cells ◽  
Transmembrane Channels

scholarly journals Droplet Microfluidics for Tumor Drug‐Related Studies and Programmable Artificial Cells

2021 ◽  
pp. 2000123
Author(s):  
Pantelitsa Dimitriou ◽  
Jin Li ◽  
Giusy Tornillo ◽  
Thomas McCloy ◽  
David Barrow
Keyword(s):  
Droplet Microfluidics ◽  
Artificial Cells

scholarly journals CryoEM structure of the antibacterial target PBP1b at 3.3 Å resolution

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Nathanael A. Caveney ◽  
Sean D. Workman ◽  
Rui Yan ◽  
Claire E. Atkinson ◽  
Zhiheng Yu ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Acid Anhydride ◽  
Atomic Resolution ◽  
Inherent Difficulty ◽  
Penicillin Binding Proteins ◽  
Antibiotic Development ◽  
Class A ◽  
Membrane Mimetic ◽  
Antibacterial Target ◽  
Resolution Structure

AbstractThe pathway for the biosynthesis of the bacterial cell wall is one of the most prolific antibiotic targets, exemplified by the widespread use of β-lactam antibiotics. Despite this, our structural understanding of class A penicillin binding proteins, which perform the last two steps in this pathway, is incomplete due to the inherent difficulty in their crystallization and the complexity of their substrates. Here, we determine the near atomic resolution structure of the 83 kDa class A PBP from Escherichia coli, PBP1b, using cryogenic electron microscopy and a styrene maleic acid anhydride membrane mimetic. PBP1b, in its apo form, is seen to exhibit a distinct conformation in comparison to Moenomycin-bound crystal structures. The work herein paves the way for the use of cryoEM in structure-guided antibiotic development for this notoriously difficult to crystalize class of proteins and their complex substrates.

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