Solid-like Dynamic Nuclear Polarization Observed in the Fluid Phase of Lipid Bilayers at 9.4 T

2022 ◽  
Author(s):  
Andrei A. Kuzhelev ◽  
Danhua Dai ◽  
Vasyl Denysenkov ◽  
Thomas F. Prisner
Keyword(s):  
Lipid Bilayers ◽  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization ◽  
Fluid Phase

scholarly journals Solid-State NMR/Dynamic Nuclear Polarization of Polypeptides in Planar Supported Lipid Bilayers

2015 ◽  
Vol 119 (46) ◽  
pp. 14574-14583 ◽  
Author(s):  
Evgeniy S. Salnikov ◽  
Hiba Sarrouj ◽  
Christian Reiter ◽  
Christopher Aisenbrey ◽  
Armin Purea ◽  
...  
Keyword(s):  
Solid State ◽  
Lipid Bilayers ◽  
Dynamic Nuclear Polarization ◽  
Solid State Nmr ◽  
Nuclear Polarization ◽  
Supported Lipid Bilayers

Dynamic Nuclear Polarization-Enhanced NMR on Aligned Lipid Bilayers at Ambient Temperature

2014 ◽  
Vol 136 (44) ◽  
pp. 15533-15536 ◽  
Author(s):  
Orawan Jakdetchai ◽  
Vasyl Denysenkov ◽  
Johanna Becker-Baldus ◽  
Bercem Dutagaci ◽  
Thomas F. Prisner ◽  
...  
Keyword(s):  
Ambient Temperature ◽  
Lipid Bilayers ◽  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization

Direct Measurement of Charge Reversal on Lipid Bilayers using Heterodyne-Detected Second Harmonic Generation Spectroscopy

2019 ◽  
Author(s):  
HanByul Chang ◽  
Paul Ohno ◽  
Yangdongling Liu ◽  
Franz Geiger
Keyword(s):  
Lipid Bilayers ◽  
Surface Potential ◽  
Second Harmonic ◽  
Fluid Phase ◽  
Cationic Polyelectrolyte ◽  
Charge Reversal ◽  
Buried Interfaces ◽  
Phase Transition Temperatures ◽  
Complementary Techniques ◽  
Order Susceptibility

We report the detection of charge reversal induced by the adsorption of a cationic polyelectrolyte, poly(allylamine) hydrochloride (PAH), to buried supported lipid bilayers (SLBs), used as idealized model biological membranes. We observe changes in the surface potential in isolation from other contributors to the total SHG response by extracting the phase-shifted potential-dependent third-order susceptibility from the overall SHG signal. We demonstrate the utility of this technique in detecting both the sign of the surface potential and the point of charge reversal at buried interfaces without any prior information or complementary techniques<i>.</i>Furthermore, isolation of the second-order susceptibility contribution from the overall SHG response allows us to directly monitor changes in the Stern Layer. Finally, we characterize the Stern and Diffuse Layers over single-component SLBs formed from three different zwitterionic lipids of different gel-to-fluid phase transition temperatures (T<sub>m</sub>s). We determine whether the surface potential changes with the physical phase state (gel, transitioning, or fluid) of the SLB and incorporate 20 percent of negatively charged lipids to the zwitterionic SLB to investigate how the surface potential changes with surface charge.

Porphyrins as Versatile, Aggregation-Tolerant, and Biocompatible Polarizing Agents for Triplet Dynamic Nuclear Polarization of Biomolecules

2021 ◽  
pp. 2645-2650
Author(s):  
Tomoyuki Hamachi ◽  
Koki Nishimura ◽  
Hironori Kouno ◽  
Yusuke Kawashima ◽  
Kenichiro Tateishi ◽  
...  
Keyword(s):  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization

Solvent-free dynamic nuclear polarization enhancements in organically modified mesoporous silica

2021 ◽  
Author(s):  
Marcos de Oliveira Jr. ◽  
Kevin Herr ◽  
Martin Brodrecht ◽  
Nadia Berenice Haro-Mares ◽  
Till Wissel ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Porous Materials ◽  
Structural Characterization ◽  
Dynamic Nuclear Polarization ◽  
High Field ◽  
Nuclear Polarization ◽  
Organically Modified ◽  
Field Dynamic ◽  
Free Dynamic

High-field Dynamic Nuclear Polarization is a powerful tool for the structural characterization of species on the surface of porous materials or nanoparticles. For these studies the main source of polarization...

scholarly journals Dynamic Nuclear Polarization of Silicon Carbide Micro- and Nanoparticles

2021 ◽  
Author(s):  
Min Lin ◽  
Vincent Breukels ◽  
Tom W. J. Scheenen ◽  
Jos M. J. Paulusse
Keyword(s):  
Silicon Carbide ◽  
Dynamic Nuclear Polarization ◽  
Nuclear Polarization

scholarly journals Dynamic Nuclear Polarization / solid-state NMR of membranes. Thermal effects and sample geometry

2019 ◽  
Vol 100 ◽  
pp. 70-76 ◽  
Author(s):  
Evgeniy Sergeevich Salnikov ◽  
Fabien Aussenac ◽  
Sebastian Abel ◽  
Armin Purea ◽  
Paul Tordo ◽  
...  
Keyword(s):  
Solid State ◽  
Dynamic Nuclear Polarization ◽  
Thermal Effects ◽  
Solid State Nmr ◽  
Nuclear Polarization ◽  
Sample Geometry
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