Enhanced Sensitivity for the Analysis of Perfluoroethercarboxylic Acids Using LC–ESI–MS/MS: Effects of Probe Position, Mobile Phase Additive, and Capillary Voltage

2020 ◽  
Vol 31 (10) ◽  
pp. 2124-2132
Author(s):  
Richard A. Brase ◽  
David C. Spink
Keyword(s):  
Mobile Phase ◽  
Probe Position ◽  
Esi Ms ◽  
Enhanced Sensitivity ◽  
Mobile Phase Additive ◽  
Capillary Voltage

DMSO as a mobile phase additive enhances detection of ubiquitination sites by nano-LC-ESI-MS/MS

2018 ◽  
Vol 53 (2) ◽  
pp. 183-187 ◽  
Author(s):  
Joerg Doellinger ◽  
Marica Grossegesse ◽  
Andreas Nitsche ◽  
Peter Lasch
Keyword(s):  
Mobile Phase ◽  
Esi Ms ◽  
Mobile Phase Additive

scholarly journals Simultaneous and Trace Level Determination of Six Potential Impurities by UPLC-ESI-MS/MS in Antiarrhythmic Drug: Dronedarone Hydrochloride

2020 ◽  
Vol 32 (5) ◽  
pp. 1183-1190
Author(s):  
K. Durga Raja ◽  
V. Saradhi Venkata Ramana ◽  
K. Raghu Babu ◽  
B. Kishore Babu ◽  
V. Jagadeesh Kumar ◽  
...  
Keyword(s):  
Mobile Phase ◽  
Gradient Elution ◽  
Ultra Performance Liquid Chromatography ◽  
Solvent Mixture ◽  
Column Temperature ◽  
Esi Ms ◽  
Quantitation Limit ◽  
Ich Guidelines ◽  
Validation Tests ◽  
Development And Validation

Development and validation of six potential impurities by ultra performance liquid chromatography electro spray ionization tandem mass (UPLC-ESI-MS/MS) method for dronedarone hydrochloride drug was accomplished coherent with ICH guidelines. Successful chromatographic separation of dronedarone with its six impurities was attained by using gradient elution mode on RP-UPLC column using three pump mode system of 0.1 % formic acid in water as mobile phase A, methanol as the mobile phase B and solvent mixture of methanol, acetonitrile and water in the ratio of 65:30:5 v/v/v as the mobile phase C. Chromatographic conditions were set as 0.3 mL min-1 flow rate at the column temperature of 45 °C with the injection volume 2 μL. Briefly, the method enabled quantitation of six impurities with high accuracy (recovery > 90 %) and precision (% RSD < 5.0),within the ranges of 0.18-2.82 μg g-1. The regression (r) for each impurity over a range was > 0.99. The detection limit and quantitation limit of impurities were set at 0.09 and 0.18 μg g-1, respectively. The performed validation tests proved the suitability of the method for its intended purposes.

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