Enzyme-Linked Immunosorbent Assay Based on a Polyclonal Antibody for the Detection of the Insecticide Fenitrothion. Evaluation of Antiserum and Application to the Analysis of Water Samples

2002 ◽  
Vol 50 (1) ◽  
pp. 53-58 ◽  
Author(s):  
Eiki Watanabe ◽  
Yukiko Kanzaki ◽  
Hiroshi Tokumoto ◽  
Ryoko Hoshino ◽  
Hiroaki Kubo ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Polyclonal Antibody ◽  
Water Samples ◽  
Enzyme Linked Immunosorbent Assay

Production of Polyclonal Antibody and Development of a Competitive Enzyme-Linked Immunosorbent Assay for Benzoic Acid in Foods

2014 ◽  
Vol 8 (5) ◽  
pp. 1101-1111 ◽  
Author(s):  
Lin Luo ◽  
Yu-Dong Shen ◽  
Hong Wang ◽  
Zhi-Li Xiao ◽  
Yuan-Ming Sun ◽  
...  
Keyword(s):  
Benzoic Acid ◽  
Immunosorbent Assay ◽  
Polyclonal Antibody ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals Novel Polyclonal Antibody Raised against Tetrodotoxin Using Its Haptenic Antigen Prepared from 4,9-anhydrotetrodotoxin Reacted with 1,2-Ethaneditiol and Further Reacted with Keyhole Limpet Hemocyanin

Toxins ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 551 ◽  
Author(s):  
Sato ◽  
Takaishi ◽  
Yasumoto ◽  
Watabe
Keyword(s):  
Immunosorbent Assay ◽  
Polyclonal Antibody ◽  
Specific Antibody ◽  
Metabolic Pathways ◽  
Keyhole Limpet Hemocyanin ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elisa System ◽  
One Step ◽  
Analytical Procedures ◽  
Specific Polyclonal Antibody

A novel polyclonal antibody against tetrodotoxin (TTX) was raised using its haptenic antigen, where 4,9-anhydroTTX was reacted with 1,2-ethanedithiol and this derivative was further reacted with keyhole limpet hemocyanin (KLH). This newly designed antigen (KLH-TTX) was inoculated into rabbits, resulting in the production of the specific polyclonal antibody, which reacted well with TTX and its analogs, 4-epiTTX, 11-oxoTTX and 5,6,11-trideoxyTTX, except for 4,9-anhydroTTX. The enzyme-linked immunosorbent assay (ELISA) system using this specific antibody was also developed in the present study. This newly developed polyclonal antibody with analytical procedures using direct one-step ELISA is useful to detect TTX and its analogs in toxic organisms and also disclose the mechanisms involved in their metabolic pathways and accumulation of TTX.

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