Induction of Apoptosis by 1-(2-Hydroxy-5-methylphenyl)-3-phenyl-1,3-propanedione through Reactive Oxygen Species Production, GADD153 Expression, and Caspases Activation in Human Epidermoid Carcinoma Cells

2005 ◽  
Vol 53 (23) ◽  
pp. 9039-9049 ◽  
Author(s):  
Min-Hsiung Pan ◽  
Yi-Hong Sin ◽  
Ching-Shu Lai ◽  
Ying-Jan Wang ◽  
Jen-Kun Lin ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen Species Production ◽  
Reactive Oxygen ◽  
Carcinoma Cells ◽  
Epidermoid Carcinoma ◽  
Oxygen Species ◽  
Induction Of Apoptosis ◽  
Species Production ◽  
Gadd153 Expression ◽  
Human Epidermoid Carcinoma

Induction of apoptosis and reactive oxygen species production byN-nitrosopiperidine andN-nitrosodibutylamine in human leukemia cells

2008 ◽  
Vol 28 (4) ◽  
pp. 455-465 ◽  
Author(s):  
Almudena García ◽  
Paloma Morales ◽  
Nuria Arranz ◽  
Eugenia Delgado ◽  
Joseph Rafter ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen Species Production ◽  
Reactive Oxygen ◽  
Leukemia Cells ◽  
Human Leukemia ◽  
Oxygen Species ◽  
Human Leukemia Cells ◽  
Induction Of Apoptosis ◽  
Species Production

Induction of apoptosis and reactive oxygen species production by N-nitrosopiperidine and N-nitrosodibutylamine in human leukemia cells

2007 ◽  
Vol 172 ◽  
pp. S66 ◽  
Author(s):  
Almudena García ◽  
Paloma Morales ◽  
Nuria Arranz ◽  
Eugenia Delgado ◽  
Ana I. Haza
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen Species Production ◽  
Reactive Oxygen ◽  
Leukemia Cells ◽  
Human Leukemia ◽  
Oxygen Species ◽  
Human Leukemia Cells ◽  
Induction Of Apoptosis ◽  
Species Production

scholarly journals Cytotoxic effect of celastrol alone or in combination with paclitaxel on anaplastic thyroid carcinoma cells

Tumor Biology ◽  
2017 ◽  
Vol 39 (5) ◽  
pp. 101042831769836 ◽  
Author(s):  
Si Hyoung Kim ◽  
Jun Goo Kang ◽  
Chul Sik Kim ◽  
Sung-Hee Ihm ◽  
Moon Gi Choi ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Thyroid Carcinoma ◽  
Cell Viability ◽  
Reactive Oxygen Species Production ◽  
Reactive Oxygen ◽  
Anaplastic Thyroid Carcinoma ◽  
Carcinoma Cells ◽  
Oxygen Species ◽  
Protein Levels ◽  
Species Production

The influence of celastrol alone or in combination with paclitaxel on survival of anaplastic thyroid carcinoma cells was investigated. In 8505C and SW1736 cells, after treatment of celastrol, cell viability decreased, and cytotoxic activity increased. The protein levels of heat shock protein (hsp) 90, hsp70, Bax, death receptor 5, cleaved caspase-3, cleaved poly (ADP-ribose) polymerase, phospho-extracellular signal-regulated kinase 1/2 (ERK1/2), and phospho-c-Jun N-terminal kinase (JNK) were elevated, and those of Bcl2, phospho-nuclear factor-kappaB (NF-κB), and total and phospho-Akt were reduced. The endoplasmic reticulum stress markers expression and reactive oxygen species production were enhanced. In celastrol-treated cells, N-acetylcysteine increased cell viability and phospho-NF-κB protein levels, and decreased reactive oxygen species production and cytotoxic activity. The protein levels of cyclooxygenase 2, phospho-ERK1/2, phospho-JNK and Bip were diminished. After treatment of both celastrol and paclitaxel, compared with paclitaxel alone, cell viability and the percentage of viable cells were reduced, and death rate and cytotoxic activity were elevated. The protein levels of phospho-ERK1/2, phospho-JNK, Bip, and cyclooxygenase 2, and reactive oxygen species production were enhanced. All of the Combination Index values calculated by Chou–Talalay equation were lower than 1.0, implying the synergism between celastrol and paclitaxel in induction of cell death. In conclusion, our results suggest that celastrol induces cytotoxicity through involvement of Bcl2 family proteins and death receptor, and modulation of phospho-NF-κB, Akt, and mitogen-activated protein kinase in association with endoplasmic reticulum stress and reactive oxygen species production in anaplastic thyroid carcinoma cells. Moreover, celastrol synergizes with paclitaxel in induction of cytotoxicity in anaplastic thyroid carcinoma cells.

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