Real-Time TaqMan Polymerase Chain Reaction Detection and Quantification of Cow DNA in Pure Water Buffalo Mozzarella Cheese: Method Validation and Its Application on Commercial Samples

2007 ◽  
Vol 55 (9) ◽  
pp. 3429-3434 ◽  
Author(s):  
Rosa M. Lopparelli ◽  
Barbara Cardazzo ◽  
Stefania Balzan ◽  
Valerio Giaccone ◽  
Enrico Novelli
Keyword(s):  
Polymerase Chain Reaction ◽  
Method Validation ◽  
Water Buffalo ◽  
Pure Water ◽  
Mozzarella Cheese ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Detection And Quantification ◽  
Taqman Polymerase Chain Reaction

Use of duplex polymerase chain reaction (duplex-PCR) technique to identify bovine and water buffalo milk used in making mozzarella cheese

2001 ◽  
Vol 68 (4) ◽  
pp. 689-698 ◽  
Author(s):  
STEFANO REA ◽  
KOICHI CHIKUNI ◽  
RAFFAELLA BRANCIARI ◽  
RAM SUKASI SANGAMAYYA ◽  
DAVID RANUCCI ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Species Identification ◽  
Water Buffalo ◽  
Bovine Milk ◽  
Buffalo Milk ◽  
Animal Origin ◽  
Duplex Pcr ◽  
Mozzarella Cheese ◽  
Chain Reaction ◽  
Polymerase Chain

Molecular biology techniques have been used for species identification in food of animal origin in relatively recent years. A polymerase chain reaction (PCR) based method, the multiplex PCR, was recently applied to species identification in meat and meat products. It allows co-amplification of separate regions of a single gene or specific fragments, each typical of a different animal species in a single PCR reaction, using different pairs of primers in the same reaction mix. In the present paper, the duplex-PCR technique is proposed to identify bovine and water buffalo DNA in a single PCR assay in milk and mozzarella cheese (a typical Italian cheese, originally made from pure water buffalo milk). Because of its lower cost, undeclared bovine milk is added to water buffalo milk for making different kinds of mozzarella cheese. The results of this experiment indicate the applicability of this method, which showed an absolute specificity for the two species and a high sensitivity even down to low DNA concentrations (1 pg). In bovine and water buffalo mixtures of both milk and mozzarella cheese, the minimum concentration tested was 1% of bovine in water buffalo milk and water buffalo in bovine milk. The importance of the somatic cell content in raw milk is also discussed with special reference to the evaluation of mixtures (milk or cheese) of the two species.

scholarly journals Polymerase chain reaction detection of cytomegalovirus genome in renal biopsies

1992 ◽  
Vol 42 (4) ◽  
pp. 1012-1016 ◽  
Author(s):  
Susan Kadereit ◽  
Susan Michelson ◽  
Beatrice Mougenot ◽  
Philippe Thibault ◽  
Pierre J. Verroust ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Renal Biopsies ◽  
Polymerase Chain

scholarly journals Polymerase chain reaction detection of sepsis-inducing pathogens in blood using SepsiTest™

Critical Care ◽  
2008 ◽  
Vol 12 (Suppl 5) ◽  
pp. P10 ◽  
Author(s):  
Claudia Disqué ◽  
Anna-Julia Kochem ◽  
Helge Mühl ◽  
Michael Lorenz ◽  
Samir Sakka
Keyword(s):  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Polymerase Chain

Polymerase Chain Reaction Detection of Salmonella spp. in Fecal Samples of Pet Birds

2008 ◽  
Vol 3 (1) ◽  
pp. e29-e29
Author(s):  
B. Sareyyüpoğlu ◽  
A Çelik Ok ◽  
Z. Cantekin ◽  
H. Yardimci ◽  
M. Akan ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Salmonella Spp ◽  
Fecal Samples ◽  
Pet Birds ◽  
Polymerase Chain
Sign in / Sign up

Export Citation Format

Share Document