Validation of a Novel Estrogen Receptor-Based Microtitration Plate Assay for the Determination of Phytoestrogens in Soy-Based Foods

1999 ◽  
Vol 47 (10) ◽  
pp. 4106-4111 ◽  
Author(s):  
Stephen D. Garrett ◽  
Heather A. Lee ◽  
Phöbe M. K. Friar ◽  
Michael R. A. Morgan
Keyword(s):  
Estrogen Receptor ◽  
Plate Assay ◽  
Microtitration Plate

Determination of Estrogen Receptors in Human Breast Cancer: Comparison between Enzyme Immunoassay and Dextran-Coated Charcoal Method

1986 ◽  
Vol 72 (5) ◽  
pp. 511-514 ◽  
Author(s):  
Cecilia Bozzetti ◽  
Nadia Naldi ◽  
Annamaria Guazzi ◽  
Rita Nizzoli ◽  
Magda Benecchi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Estrogen Receptor ◽  
Estrogen Receptors ◽  
Enzyme Immunoassay ◽  
Strong Correlation ◽  
Human Breast Cancer ◽  
Human Breast ◽  
Dextran Coated Charcoal ◽  
The Mean

Estrogen receptor determination was performed on 120 breast cancer cytosols, using the dextran-coated charcoal method (DCC) and an enzyme immunoassay (EIA) to compare the efficiency of the two techniques. A strong correlation was noted between ER concentrations determined by DCC and EIA (P < 0.001). The mean ER-EIA value was significantly higher than the mean ER-DCC value in premenopausal (P < 0.001) as well in postmenopausal (P < 0.001) patients.

scholarly journals Assessment of Interlaboratory Variation in the Immunohistochemical Determination of Estrogen Receptor Status Using a Breast Cancer Tissue Microarray

2002 ◽  
Vol 117 (5) ◽  
pp. 723-728 ◽  
Author(s):  
Robin L. Parker ◽  
David G. Huntsman ◽  
David W. Lesack ◽  
James B. Cupples ◽  
Dennis R. Grant ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Estrogen Receptor ◽  
Tissue Microarray ◽  
Estrogen Receptor Status ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Receptor Status

The relevance of kinetic parameters in the determination of specific binding to the estrogen receptor

1978 ◽  
Vol 9 (1) ◽  
pp. 9-15 ◽  
Author(s):  
M.M. Bouton ◽  
J.P. Raynaud
Keyword(s):  
Estrogen Receptor ◽  
Kinetic Parameters ◽  
Specific Binding

Estrogen Receptor Immunocytochemistry for Preoperative Determination of Estrogen Receptor Status on Fine-Needle Aspirates of Breast Cancer

1987 ◽  
Vol 88 (4) ◽  
pp. 399-404 ◽  
Author(s):  
Angelika Reiner ◽  
Georg Reiner ◽  
Jürgen Spona ◽  
Bela Teleky ◽  
Roland Kolb ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Estrogen Receptor ◽  
Estrogen Receptor Status ◽  
Fine Needle ◽  
Receptor Status ◽  
Fine Needle Aspirates

scholarly journals Study of RsaI polymorphism of the ERβ gene in Greek women with endometriosis

2020 ◽  
Vol 19 (3) ◽  
pp. 115-122
Author(s):  
Elli Anagnostou ◽  
Agathi Theodoropoulou ◽  
Despina Mavrogianni ◽  
Athanasios Protopapas ◽  
Peter Drakakis ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Control Group ◽  
Genetic Profile ◽  
Genotype Distribution ◽  
Female Population ◽  
Infertile Women ◽  
Caucasian Origin ◽  
Rsai Polymorphism ◽  
Esr2 Gene

Estrogens and estrogen receptors (ERs) play an important role in the pathogenesis of endometriosis. The aim of this study was to investigate the presence of gene polymorphism RsaI in the gene of the estrogen receptor ERβ in the Greek female population, and its distribution in women suffering from endometriosis and in a control group. We included 67 consecutive infertile women of Caucasian origin who were operated laparoscopically in our Gynecological Endoscopy Unit for endometriosis, and 96 women participated as control group. Patients were genotyped for RsaI (G/A, rs1256049) polymorphism in ESR2 exon 5, using real-time PCR. The patients’ genotype distribution did not differ from the control group. There were no women homozygous for the polymorphic allele in neither group. The different genotypes of ESR2 could not be associated with the stage of endometriosis. The data of this study point that in Greek population who had proven endometriosis the determination of RsaI polymorphism of ESR2 gene doesn’t offer any information for the progression of endometriosis, regarding the genetic profile of this particular gene.

scholarly journals Plate Assay for Simultaneous Detection of Alginate Lyases and Determination of Substrate Specificity

1990 ◽  
Vol 56 (7) ◽  
pp. 2265-2267 ◽  
Author(s):  
Peter Gacesa ◽  
Frederick S. Wusteman
Keyword(s):  
Substrate Specificity ◽  
Simultaneous Detection ◽  
Plate Assay ◽  
Alginate Lyases

Microbiological Plate Assay for Determination of Tilmicosin in Bovine Serum

1995 ◽  
Vol 78 (3) ◽  
pp. 659-662 ◽  
Author(s):  
Mark R Coleman ◽  
James S Peloso ◽  
John W Moran
Keyword(s):  
Bovine Serum ◽  
Limit Of Detection ◽  
Agar Plate ◽  
Treated Animal ◽  
Validation Data ◽  
Method Performance ◽  
Plate Assay ◽  
Relative Standard ◽  
Limit Of Quantitation

Abstract A microbiological agar plate assay is described for determination of tilmicosin in bovine blood serum. The serum or serum dilution is added directly to wells cut in the agar plates. Tilmicosin activity is determined by measuring the zone of bacterial growth inhibition in agar medium inoculated with Micrococcus luteus, ATCC 9341. The assay was validated by evaluating the following parameters: accuracy, precision, linearity, parallelism, ruggedness, storage stability, and relative activity of isomers. Accuracy was evaluated with freshly collected bovine serum and with commercially available sera. Recoveries ranged from 93.4 to 97.5% across a fortification range of 0.08 to 1.28 μg/mL. Precision was estimated over a 6-day period with serum obtained from a tilmicosin-treated animal. Relative standard deviations were 0.63 to 3.13% within day and 5.23% across 6 days. Standard curves were linear with little variation in slope. No parallelism was observed between tilmicosin in serum and tilmicosin in buffered saline. The limit of detection was estimated to be 0.05 μg/mL, and the validated limit of quantitation was 0.08 μg/mL. Ruggedness was evaluated with different lots of antibiotic medium, different lots of sera, and different analysts. These variables did not affect method performance. Analyses of tilmicosin in frozen sera demonstrated that tilmicosin is stable for up to 16 days when stored at −20°C. A comparison of the relative microbiological activities of the purified cis and trans isomers of tilmicosin to that of the reference standard indicated no differences in microbiological activities, and showed a parallel response among the 3. The validation data demonstrate that this method is a rugged, reliable, and simple assay for tilmicosin in serum.

Development of 96-microwell plate assay with Fluorescence Reader and HPLC method with Fluorescence Detection for High-throughput Analysis of Linifanib in its Bulk and Dosage Forms

2020 ◽  
Vol 17 ◽  
Author(s):  
Nasr Y. Khalil ◽  
Ibrahim A. Darwish ◽  
Mamdouh Alanazi ◽  
Mohammed A. Hamidaddin
Keyword(s):  
Fluorescence Detection ◽  
High Performance ◽  
Kinase Inhibitor ◽  
Internal Standard ◽  
Dosage Forms ◽  
Fluorescence Detector ◽  
High Throughput Analysis ◽  
Plate Assay ◽  
Microwell Plate

Background: Linifanib (LFB) is a tyrosine kinase inhibitor with antineoplastic activity. The existing methods for analysis of LFB in bulk and dosage forms do not meet the requirements of quality control (QC) analysis. Objective: The present study was devoted to the development of two methods with high throughputs for determination of LFB. These methods are 96-microwell plate assay with microplate fluorescence reader (MWP-FR) and high-performance liquid chromatography with fluorescence detection (HPLC-FD). Methods: The MWP-FR assay was carried out in white opaque 96-well assay plates and the native fluorescence signals of LFB were measured at 360 nm for excitation and 500 nm for emission. In the HPLC-FD, the chromatographic separation of LFB and quinine sulphate (QS) as internal standard (IS) was performed on μ-Bondapack CN HPLC column using a mobile phase consisting of acetonitrile:water (60:40, v/v) pumped at a flow rate of 1 ml/min in an isocratic mode. The fluorescence detector was set at 350 nm for excitation and 454 nm for emission. Results: The linear ranges of the MWP-FR and HPLC-FD were 1 – 12 μg/well and 10 – 500 ng/ml, respectively. The limits of quantitation were 0.85 μg/well and 8.24 ng/ml for MWP-FR and HPLC-FD, respectively. Both MWP-FR and HPLC-FL methods were successfully applied for the determination of LFB in both bulk and tablets. Conclusion: Both methods have high analytical throughputs, they are suitable for use in QC laboratories for analysis of large numbers of LFB samples, and are environmentally friendly as they consume low volumes of chemicals and solvents.

A Case of Delayed Cutaneous Metastases. A Determination of the Estrogen Receptor and Cellular Proliferating Activities.

1998 ◽  
Vol 60 (5) ◽  
pp. 643-647 ◽  
Author(s):  
Tomoyoshi OKADA ◽  
Hiroyuki HARA ◽  
Fuminori WAKUI ◽  
Hiroyuki SHIMOJIMA ◽  
Michio HONJO ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Cutaneous Metastases
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