Direct Counting of Submicrometer-Sized Photosynthetic Apparatus Dispersed in Medium at Cryogenic Temperature by Confocal Laser Fluorescence Microscopy:  Estimation of the Number of Bacteriochlorophyllcin Single Light-Harvesting Antenna Complexes Chlorosomes of Green Photosynthetic Bacteria

2007 ◽  
Vol 111 (43) ◽  
pp. 12605-12609 ◽  
Author(s):  
Yoshitaka Saga ◽  
Yutaka Shibata ◽  
Shigeru Itoh ◽  
Hitoshi Tamiaki
Keyword(s):  
Fluorescence Microscopy ◽  
Cryogenic Temperature ◽  
Photosynthetic Bacteria ◽  
Light Harvesting ◽  
Photosynthetic Apparatus ◽  
Laser Fluorescence ◽  
Confocal Laser ◽  
Green Photosynthetic Bacteria ◽  
Direct Counting ◽  
Light Harvesting Antenna

Various adhesion molecules impair microvascular leukocyte kinetics in ventilator-induced lung injury

2006 ◽  
Vol 290 (6) ◽  
pp. L1059-L1068 ◽  
Author(s):  
Naoki Miyao ◽  
Yukio Suzuki ◽  
Kei Takeshita ◽  
Hiroyasu Kudo ◽  
Makoto Ishii ◽  
...  
Keyword(s):  
Lung Injury ◽  
Fluorescence Microscopy ◽  
Adhesion Molecules ◽  
Mononuclear Cells ◽  
Tissue Injury ◽  
Laser Fluorescence ◽  
Confocal Laser ◽  
Polymorphonuclear Cells ◽  
Ventilator Induced Lung Injury ◽  
Endothelial Adhesion Molecules

Although the endothelial expression of various adhesion molecules substantially differs between pulmonary microvessels, their importance for neutrophil and lymphocyte sequestration in ventilator-induced lung injury (VILI) has not been systematically analyzed. We investigated the kinetics of polymorphonuclear cells (PMN) and mononuclear cells (MN) in the acinar microcirculation of the isolated rat lung with VILI by real-time confocal laser fluorescence microscopy, with or without inhibition of ICAM-1, VCAM-1, or P-selectin by monoclonal antibodies (MAb). Adhesion molecules in each microvessel were estimated by intravital fluorescence microscopy or immunohistochemical staining. In high tidal volume-ventilated lungs, 1) ICAM-1, VCAM-1, and P-selectin were differently upregulated in venules, arterioles, and capillaries; 2) venular PMN rolling was improved by inhibition of ICAM-1, VCAM-1, or P-selectin, whereas arteriolar PMN rolling was improved by ICAM-1 or VCAM-1 inhibition; 3) capillary PMN entrapment was ameliorated only by anti-ICAM-1 MAb; and 4) MN rolling in venules and arterioles and MN entrapment in capillaries were improved by ICAM-1 and VCAM-1 inhibition. In conclusion, the contribution of endothelial adhesion molecules to abnormal leukocyte behavior in VILI-injured microcirculation is microvessel and leukocyte specific. ICAM-1- and VCAM-1-dependent, but P-selectin-independent, arteriolar PMN rolling, which is expected to reflect the initial stage of tissue injury, should be taken as a phenomenon unique to ventilator-associated lung injury.

Absorption Linear Dichroism Measured Directly on a Single Light-Harvesting System: The Role of Disorder in Chlorosomes of Green Photosynthetic Bacteria

2011 ◽  
Vol 133 (17) ◽  
pp. 6703-6710 ◽  
Author(s):  
Shu Furumaki ◽  
Frantisek Vacha ◽  
Satoshi Habuchi ◽  
Yusuke Tsukatani ◽  
Donald A. Bryant ◽  
...  
Keyword(s):  
Photosynthetic Bacteria ◽  
Light Harvesting ◽  
Linear Dichroism ◽  
Green Photosynthetic Bacteria

scholarly journals Bacteriochlorophyll-protein complexes from the light-harvesting antenna of photosynthetic bacteria

Biochemistry ◽  
1978 ◽  
Vol 17 (10) ◽  
pp. 2011-2019 ◽  
Author(s):  
Kenneth Sauer ◽  
Lynn A. Austin
Keyword(s):  
Photosynthetic Bacteria ◽  
Light Harvesting ◽  
Protein Complexes ◽  
Light Harvesting Antenna

Exciton interactions in the light-harvesting antenna of photosynthetic bacteria studied with triplet-singlet spectroscopy and singlet-triplet annihilation on the B820 subunit form of Rhodospirillum rubrum

1991 ◽  
Vol 1059 (1) ◽  
pp. 111-119 ◽  
Author(s):  
Frank van Mourik ◽  
Cornelis J.R. van der Oord ◽  
Kees J. Visscher ◽  
Pamela S. Parkes-Loach ◽  
Paul A. Loach ◽  
...  
Keyword(s):  
Photosynthetic Bacteria ◽  
Rhodospirillum Rubrum ◽  
Light Harvesting ◽  
Exciton Interactions ◽  
Light Harvesting Antenna

scholarly journals Initial Characterization of the Photosynthetic Apparatus of “Candidatus Chlorothrix halophila,” a Filamentous, Anoxygenic Photoautotroph

2007 ◽  
Vol 189 (11) ◽  
pp. 4196-4203 ◽  
Author(s):  
Allison M. L. van de Meene ◽  
Tien Le Olson ◽  
Aaron M. Collins ◽  
Robert E. Blankenship
Keyword(s):  
Photosynthetic Bacteria ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Photosynthetic Apparatus ◽  
Fluorescence Emission ◽  
Photosynthetic Characteristics ◽  
Green Bacteria ◽  
Green Photosynthetic Bacteria ◽  
Antenna Complex ◽  
Membrane Bound

ABSTRACT “Candidatus Chlorothrix halophila” is a recently described halophilic, filamentous, anoxygenic photoautotroph (J. A. Klappenbach and B. K. Pierson, Arch. Microbiol. 181:17-25, 2004) that was enriched from the hypersaline microbial mats at Guerrero Negro, Mexico. Analysis of the photosynthetic apparatus by negative staining, spectroscopy, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the photosynthetic apparatus in this organism has similarities to the photosynthetic apparatus in both the Chloroflexi and Chlorobi phyla of green photosynthetic bacteria. The chlorosomes were found to be ellipsoidal and of various sizes, characteristics that are comparable to characteristics of chlorosomes in other species of green photosynthetic bacteria. The absorption spectrum of whole cells was dominated by the chlorosome bacteriochlorophyll c (BChl c) peak at 759 nm, with fluorescence emission at 760 nm. A second fluorescence emission band was observed at 870 nm and was tentatively attributed to a membrane-bound antenna complex. Fluorescence emission spectra obtained at 77 K revealed another complex that fluoresced at 820 nm, which probably resulted from the chlorosome baseplate complex. All of these results suggest that BChl c is present in the chlorosomes of “Ca. Chlorothrix halophila,” that BChl a is present in the baseplate, and that there is a membrane-bound antenna complex. Analysis of the proteins in the chlorosomes revealed an ∼6-kDa band, which was found to be related to the BChl c binding protein CsmA found in other green bacteria. Overall, the absorbance and fluorescence spectra of “Ca. Chlorothrix halophila” revealed an interesting mixture of photosynthetic characteristics that seemed to have properties similar to properties of both phyla of green bacteria when they were compared to the photosynthetic characteristics of Chlorobium tepidum and Chloroflexus aurantiacus.

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