Resonance Enhancement of Two-Photon Absorption in Fluorescent Proteins

2007 ◽  
Vol 111 (50) ◽  
pp. 14051-14054 ◽  
Author(s):  
M. Drobizhev ◽  
N. S. Makarov ◽  
T. Hughes ◽  
A. Rebane
Keyword(s):  
Fluorescent Proteins ◽  
Photon Absorption ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Resonance Enhancement

scholarly journals Multiphoton Bleaching of Red Fluorescent Proteins and the Ways to Reduce It

2022 ◽  
Vol 23 (2) ◽  
pp. 770
Author(s):  
Mikhail Drobizhev ◽  
Rosana S. Molina ◽  
Jacob Franklin
Keyword(s):  
Chemical Structure ◽  
Fluorescent Proteins ◽  
Multiphoton Ionization ◽  
Excitation Power ◽  
Photon Absorption ◽  
Green Fluorescent Proteins ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Red Fluorescent Proteins ◽  
Green Fluorescent

Red fluorescent proteins and biosensors built upon them are potentially beneficial for two-photon laser microscopy (TPLM) because they can image deeper layers of tissue, compared to green fluorescent proteins. However, some publications report on their very fast photobleaching, especially upon excitation at 750–800 nm. Here we study the multiphoton bleaching properties of mCherry, mPlum, tdTomato, and jREX-GECO1, measuring power dependences of photobleaching rates K at different excitation wavelengths across the whole two-photon absorption spectrum. Although all these proteins contain the chromophore with the same chemical structure, the mechanisms of their multiphoton bleaching are different. The number of photons required to initiate a photochemical reaction varies, depending on wavelength and power, from 2 (all four proteins) to 3 (jREX-GECO1) to 4 (mCherry, mPlum, tdTomato), and even up to 8 (tdTomato). We found that at sufficiently low excitation power P, the rate K often follows a quadratic power dependence, that turns into higher order dependence (K~Pα with α > 2) when the power surpasses a particular threshold P*. An optimum intensity for TPLM is close to the P*, because it provides the highest signal-to-background ratio and any further reduction of laser intensity would not improve the fluorescence/bleaching rate ratio. Additionally, one should avoid using wavelengths shorter than a particular threshold to avoid fast bleaching due to multiphoton ionization.

scholarly journals Two-Photon Absorption Properties of Chromophores of a Few Fluorescent Proteins: a Theoretical Investigation

2016 ◽  
Vol 32 (1) ◽  
pp. 301-312
Author(s):  
Chuan-Xiang YE ◽  
◽  
Hui-Li MA ◽  
Wan-Zhen LIANG ◽  
◽  
...  
Keyword(s):  
Theoretical Investigation ◽  
Fluorescent Proteins ◽  
Photon Absorption ◽  
Absorption Properties ◽  
Two Photon Absorption ◽  
Two Photon

scholarly journals Impact of the Subunit Arrangement on the Nonlinear Absorption Properties of Organometallic Complexes with Ruthenium(II) σ-Acetylide and Benzothiadiazole as Building Units §

Inorganics ◽  
2019 ◽  
Vol 7 (5) ◽  
pp. 67
Author(s):  
Eleonora Garoni ◽  
Alessia Colombo ◽  
Kenji Kamada ◽  
Claudia Dragonetti ◽  
Dominique Roberto
Keyword(s):  
Electron Acceptor ◽  
Cross Sections ◽  
Nonlinear Absorption ◽  
Organometallic Complexes ◽  
Photon Absorption ◽  
Absorption Cross ◽  
Absorption Properties ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Resonance Enhancement

In this paper, the nonlinear absorption properties of two complexes consisting of Ru(C≡CPh)(C≡C)(dppe)2 (dppe = Ph2PCH2CH2PPh2) as electron donor (D) and 4,7-di(2-thienyl)benzo[c][1,2,5]thiadiazole as electron acceptor (A) units in two different arrangement, i.e., A–D–A and D–A–D, are presented. They were measured in solution by the femtosecond open-aperture Z-scan method. The complexes show moderate two-photon absorption cross-sections σ(2) of several hundred to one thousand GM (here 1 GM = 10−50 cm4 s molecule−1 photon−1). Although they are formed by the same building units, it was found that the two-photon absorption values of the D–A–D arrangement are six times higher than that of the A–D–A one. This difference can be explained by the number of metal cores (one or two ruthenium centers), the geometrical configurations of the complexes (more or less planar), and the resonance enhancement by lowering the intermediate state.

Resonance Enhancement of the Two-Photon Absorption in PbS Quantum Dots

2010 ◽  
Author(s):  
Gero Nootz ◽  
Lazaro A. Padilha ◽  
Scott Webster ◽  
David J. Hagan ◽  
Eric W. Van Stryland ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Photon Absorption ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Resonance Enhancement ◽  
Pbs Quantum Dots

scholarly journals Absolute Two-Photon Absorption Spectra and Two-Photon Brightness of Orange and Red Fluorescent Proteins

2009 ◽  
Vol 113 (4) ◽  
pp. 855-859 ◽  
Author(s):  
M. Drobizhev ◽  
S. Tillo ◽  
N. S. Makarov ◽  
T. E. Hughes ◽  
A. Rebane
Keyword(s):  
Absorption Spectra ◽  
Fluorescent Proteins ◽  
Photon Absorption ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Red Fluorescent Proteins

scholarly journals Resonance enhancement of two photon absorption by magnetically trapped atoms in strong rf-fields

2018 ◽  
Vol 382 (4) ◽  
pp. 157-161
Author(s):  
A. Chakraborty ◽  
S.R. Mishra
Keyword(s):  
Photon Absorption ◽  
Trapped Atoms ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Magnetically Trapped ◽  
Resonance Enhancement

Soluble meso-tetrakis(arylethynyl)porphyrins — synthesis and optical properties

2014 ◽  
Vol 18 (10n11) ◽  
pp. 998-1013 ◽  
Author(s):  
Agnieszka Nowak-Król ◽  
Łukasz G. Łukasiewicz ◽  
Joy E. Haley ◽  
Mikhail Drobizhev ◽  
Aleksander Rebane ◽  
...  
Keyword(s):  
Optical Properties ◽  
Red Light ◽  
Photon Absorption ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Resonance Enhancement ◽  
Order Of Magnitude ◽  
Homo And Lumo ◽  
The One ◽  
First Time

Trans- A 2 B 2-tetrakis(arylethynyl)porphyrins with suitable solubility in CH 2 Cl 2, CHCl 3, EtOAc , acetone and toluene have been obtained for the first time. Among two possible strategies the one comprising the synthesis of 5,15-dibromo-10,20-bis[(isopropylsilyl)ethynyl]porphyrin proved to be more efficient. The pathway towards densely substituted arylacetylenes has been optimized. The use of previously identified 3,4,5-trialkoxyaryl substituent was crucial for achieving the reasonable solubility. The optical properties of meso-substituted tetrakis(arylethynyl)porphyrins were studied showing that strong polarization imparted by direct conjugation of all four substituents with porphyrin core resulted not only in strong absorption of red light but also in a relatively long triplet lifetime. Meso-tetrakis(arylethynyl)porphyrins have a significantly longer lifetime of T1 state than bis(arylethynyl)porphyrins and in their case all the states are mixtures of transitions between the HOMO-1, HOMO and LUMO, LUMO+1 MOs. We show that the presence of two additional arylethynyl substituents at meso-positions enhance the maximum two-photon absorption cross-section of trans- A 2 B 2-tetrakis(arylethynyl)porphyrins by more than one order of magnitude. Maximum values as high as σ2 = 500 GM at 950 nm result from realization of suitable conditions for effective resonance enhancement along with a lowering of the energy and intensification of the two-photon allowed transitions in the Soret region.

Selective excitation of fluorescent proteins on the basis of the two-photon absorption spectrum measurement

2006 ◽  
Author(s):  
M. Tanaka ◽  
J. Tada ◽  
F. Kannari ◽  
H. Kawano ◽  
H. Mizuno ◽  
...  
Keyword(s):  
Absorption Spectrum ◽  
Fluorescent Proteins ◽  
Selective Excitation ◽  
Photon Absorption ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Spectrum Measurement
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