Abnormal Micellar Growth in Sugar-Based and Ethoxylated Nonionic Surfactants and Their Mixtures in Dilute Regimes Using Analytical Ultracentrifugation

Formation of viscoelastic wormlike micelles in aqueous systems of mixed amino acid based anionic and nonionic surfactants in presence of salt is presented. N-dodecylglutamic acid (LAD) upon neutralization with L-Lysine in 1:2 mole ratio forms globular type of micelles above critical micelle concentration and the solution follows Newtonian fluid like behavior at 25ºC. The addition of tri(oxyethylene) nmonotetradecyl ether (C14EO3) to the semi-dilute aqueous solution of the LADLysine-2 induces one dimensional micellar growth and viscosity of solution increases. After a certain concentration of C14EO3 micelles get entangled and form a rigid network of wormlike micelles and viscosity increases by several orders of magnitude. The wormlike micellar solutions show a viscoelastic character and can be described the Maxwell mechanical model with a single stress relaxation. Rheological measurements have shown that zero shear viscosity (No) increases with the C14EO3 concentration gradually at first sharpl, and then finally decreases before phase separation.Keywords: nonionic surfactant, wormlike micelles, rheology, viscoelastic solutionsDOI: 10.3126/jncs.v23i0.2098J. Nepal Chem. Soc., Vol. 23, 2008/2009 Page: 65-73

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Neutron Small Angle Scattering Studies of Micellar Growth in Mixed Anionic-Nonionic Surfactants, Sodium Dodecyl Sulfate, SDS, and Hexaethylene Glycol Monododecyl Ether, C12E6, in the Presence and Absence of Solubilized Alkane, Hexadecane

The Journal of Physical Chemistry B ◽

10.1021/jp013810l ◽

2002 ◽

Vol 106 (34) ◽

pp. 8891-8897 ◽

Cited By ~ 26

Author(s):

J. Penfold ◽

E. Staples ◽

I. Tucker

Keyword(s):

Sodium Dodecyl Sulfate ◽

Small Angle ◽

Nonionic Surfactants ◽

Sodium Dodecyl ◽

Small Angle Scattering ◽

Angle Scattering ◽

Micellar Growth ◽

Dodecyl Sulfate ◽

Presence And Absence ◽

Neutron Small Angle Scattering

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Micellar growth of poly(oxyethylene) nonionic surfactants with increasing temperature: deduction from critical micellization concentration-temperature relationships

Langmuir ◽

10.1021/la00097a005 ◽

1990 ◽

Vol 6 (7) ◽

pp. 1225-1228 ◽

Cited By ~ 7

Author(s):

Nagamune Nishikido

Keyword(s):

Nonionic Surfactants ◽

Critical Micellization Concentration ◽

Micellar Growth ◽

Increasing Temperature

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The Nature of Rapidly Extracted Phycocyanin from the Blue-Green Alga Plectonema Boryanum

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100065870 ◽

1971 ◽

Vol 29 ◽

pp. 366-367

Author(s):

M. Kessel ◽

R. MacColl

Keyword(s):

Self Assembly ◽

Analytical Ultracentrifugation ◽

Uranyl Acetate ◽

The Self ◽

Major Protein ◽

Subunit Structure ◽

Blue Green Alga ◽

Thin Sections ◽

Blue Green Algae ◽

Cacodylate Buffer

The major protein of the blue-green algae is the biliprotein, C-phycocyanin (Amax = 620 nm), which is presumed to exist in the cell in the form of distinct aggregates called phycobilisomes. The self-assembly of C-phycocyanin from monomer to hexamer has been extensively studied, but the proposed next step in the assembly of a phycobilisome, the formation of 19s subunits, is completely unknown. We have used electron microscopy and analytical ultracentrifugation in combination with a method for rapid and gentle extraction of phycocyanin to study its subunit structure and assembly.To establish the existence of phycobilisomes, cells of P. boryanum in the log phase of growth, growing at a light intensity of 200 foot candles, were fixed in 2% glutaraldehyde in 0.1M cacodylate buffer, pH 7.0, for 3 hours at 4°C. The cells were post-fixed in 1% OsO4 in the same buffer overnight. Material was stained for 1 hour in uranyl acetate (1%), dehydrated and embedded in araldite and examined in thin sections.

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Solution properties of mixed surfactant system (II)Electric properties of anionic-nonionic surfactants in aqueous solutions

Journal of Colloid and Interface Science ◽

10.1016/s0021-9797(84)80011-9 ◽

1984 ◽

Vol 98 (1) ◽

pp. 78-83 ◽

Cited By ~ 1

Author(s):

K OGINO ◽

N TSUBAKI ◽

M ABE

Keyword(s):

Aqueous Solutions ◽

Nonionic Surfactants ◽

Electric Properties ◽

Solution Properties ◽

Mixed Surfactant ◽

Mixed Surfactant System ◽

Surfactant System

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Bovine Platelet Proteins II. Purification of Platelet Fibrinogen

Thrombosis and Haemostasis ◽

10.1055/s-0038-1653556 ◽

1969 ◽

Vol 21 (03) ◽

pp. 419-427 ◽

Cited By ~ 5

Author(s):

N. O Solum ◽

S Łopaciuk

Keyword(s):

Analytical Ultracentrifugation ◽

Insoluble Fraction ◽

Disc Electrophoresis ◽

Plasma Fibrinogen ◽

Starch Gel Electrophoresis ◽

Freezing And Thawing ◽

Insoluble Material ◽

High Resolution Power ◽

Starch Gel ◽

Platelet Proteins

Summary1. Platelet fibrinogen has been purified from washed bovine platelets. The procedure was based on the methods for purification of plasma fibrinogen by fractionated precipitations and extractions with ethanol and glycine below 0°, and precipitation of proteins by dimethylformamide at 0°.2. The platelet extract obtained by freezing and thawing of the cells, freed from insoluble material by centrifugation at 23,000 x g for 30 min, contained 0.22 ±0.003mg fibrinogen per 109 platelets. Total protein of this fraction was 0.77 ±0.08 mg per 109 platelets whereas that of the insoluble fraction was 0.79 ±0.09 mg per 109 platelets.3. The most purified platelet fibrinogen fraction contained 91-98% of the protein in a thrombin-clottable state. The yield was approx. 20%. It showed homogeneity in analytical ultracentrifugation, in immunoelectrophoresis using an antiserum produced by immunization of rabbits against platelet extract, and in starch gel electrophoresis using a discontinuous system of Tris HCl and borate buffers offering a high resolution power towards the platelet proteins. Polyacrylamide disc electrophoresis revealed two to three faint lines behind the main fibrinogen line. At least one such line was also observed with purified plasma fibrinogen.

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A Cross-Method Comparison of Sub-10 nm Nanoparticle Size Distribution

10.26434/chemrxiv.9891992.v1 ◽

2019 ◽

Cited By ~ 1

Author(s):

Ye Yang ◽

Suiyang Liao ◽

Zhi Luo ◽

Runzhang Qi ◽

Niamh Mac Fhionnlaoich ◽

...

Keyword(s):

Size Distribution ◽

Analytical Ultracentrifugation ◽

Statistical Significance ◽

Method Comparison ◽

Size Determination ◽

Size Dependent ◽

X Ray Scattering ◽

Transmission Electron ◽

Gold Nps ◽

Reliable Representation

Accurate nanoparticle (NP) size determination is essential across research domains, with many functions in nanoscience and biomedical research being size-dependent. Although transmission electron microscopy (TEM) is capable of resolving a single NP down to the sub-nm scale, the reliable representation of entire populations is plagued by challenges in providing statistical significance, predominantly due to limited sample counts, suboptimal preparation procedures and operator bias during image acquisition and analysis. Meanwhile alternative techniques exist, but reliable implementation requires a detailed understanding of appendant limitations. Herein, conventional TEM is compared to the size determination of sub-10 nm gold NPs in solution by small-angle X-ray scattering and analytical ultracentrifugation. Form-free Monte Carlo fitting of scattering profiles offers access to a direct representation of the core size distribution while ultracentrifugation sedimentation velocity analysis provides information of the hydrodynamic size distribution. We report a comparison of these three methods in determining the size of quasi-monodisperse, polydisperse and bimodal gold nanoparticles of 2 – 7 nm and discuss advantages and limitations of each technique.

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