scholarly journals Large Scale Production of Vesicles by Hollow Fiber Extrusion: A Novel Method for Generating Polymersome Encapsulated Hemoglobin Dispersions

Langmuir ◽  
2010 ◽  
Vol 26 (7) ◽  
pp. 5279-5285 ◽  
Author(s):  
Shahid Rameez ◽  
Ibrahim Bamba ◽  
Andre F. Palmer
Keyword(s):  
Hollow Fiber ◽  
Large Scale ◽  
Scale Production ◽  
Large Scale Production ◽  
Novel Method

Use of a hollow fiber bioreactor for large-scale production of α2-adrenoceptors in mammalian cells

1994 ◽  
Vol 37 (2) ◽  
pp. 179-184 ◽  
Author(s):  
Sari Ala-Uotila ◽  
Anne Marjamäki ◽  
Marja-Terttu Matikainen ◽  
Markku Jalkanen
Keyword(s):  
Hollow Fiber ◽  
Mammalian Cells ◽  
Large Scale ◽  
Scale Production ◽  
Α2 Adrenoceptors ◽  
Hollow Fiber Bioreactor ◽  
Large Scale Production

scholarly journals Large-scale production of lentiviral vector in a closed system hollow fiber bioreactor

2015 ◽  
Vol 2 ◽  
pp. 15020 ◽  
Author(s):  
Jonathan Sheu ◽  
Jim Beltzer ◽  
Brian Fury ◽  
Katarzyna Wilczek ◽  
Steve Tobin ◽  
...  
Keyword(s):  
Closed System ◽  
Hollow Fiber ◽  
Large Scale ◽  
Lentiviral Vector ◽  
Scale Production ◽  
Hollow Fiber Bioreactor ◽  
Large Scale Production

A Novel Method for Expression and Large-Scale Production of Human Brain -Glutamate Decarboxylase

2000 ◽  
Vol 267 (3) ◽  
pp. 777-782 ◽  
Author(s):  
Kathleen M. Davis ◽  
Todd Foos ◽  
C.Sean Bates ◽  
Erin Tucker ◽  
Che-Chang Hsu ◽  
...  
Keyword(s):  
Human Brain ◽  
Glutamate Decarboxylase ◽  
Large Scale ◽  
Scale Production ◽  
Large Scale Production ◽  
Novel Method

scholarly journals A Novel Method for High-Level Production of TEV Protease by Superfolder GFP Tag

2009 ◽  
Vol 2009 ◽  
pp. 1-8 ◽  
Author(s):  
Xudong Wu ◽  
Di Wu ◽  
Zhisheng Lu ◽  
Wentao Chen ◽  
Xiaojian Hu ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Large Scale ◽  
Fluorescent Protein ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Scale Production ◽  
Sequence Specificity ◽  
Tev Protease ◽  
Large Scale Production ◽  
Novel Method

Because of its stringent sequence specificity, tobacco etch virus (TEV) protease is widely used to remove fusion tags from recombinant proteins. Due to the poor solubility of TEV protease, many strategies have been employed to increase the expression level of this enzyme. In our work, we introduced a novel method to produce TEV protease by using visible superfolder green fluorescent protein (sfGFP) as the fusion tag. The soluble production and catalytic activity of six variants ofsfGFP-TEV was examined, and then the best variant was selected for large-scale production. After purified by Ni-NTA affinity chromatography and Q anion exchange chromatography, the best variant ofsfGFP-TEV fusion protease was obtained with purity of over 98% and yield of over 320 mg per liter culture. ThesfGFP-TEV had a similar catalytic activity to that of the original TEV protease. Our research showed a novel method of large-scale production of visible and functional TEV protease for structural genomics research and other applications.

Large-scale production of high-quality graphene using glucose and ferric chloride

2014 ◽  
Vol 5 (12) ◽  
pp. 4656-4660 ◽  
Author(s):  
Binbin Zhang ◽  
Jinliang Song ◽  
Guanying Yang ◽  
Buxing Han
Keyword(s):  
Ferric Chloride ◽  
Large Scale ◽  
Scale Production ◽  
High Quality ◽  
Large Scale Production ◽  
Novel Method

A novel method to prepare high-quality graphene is developed using simple calcination of a mixture of glucose and FeCl3.

A low-cost automated device incorporating a hollow fiber filtration cartridge for large-scale production of ghosts from human erythrocytes

1990 ◽  
Vol 21 (4) ◽  
pp. 299-309 ◽  
Author(s):  
Soumaya El Ouggouti ◽  
Alain Truskolaski ◽  
Annette Bussel ◽  
Dodier Dhermy ◽  
Olivier Bertrand
Keyword(s):  
Hollow Fiber ◽  
Large Scale ◽  
Low Cost ◽  
Human Erythrocytes ◽  
Scale Production ◽  
Large Scale Production

Large-Scale Production of Natural Cytokines During Activation and Expansion of Human T Lymphocytes in Hollow Fiber Bioreactor Cultures

1999 ◽  
Vol 22 (4) ◽  
pp. 299-307 ◽  
Author(s):  
Cor H. J. Lamers ◽  
Jan W. Gratama ◽  
Barbara Luider-Vrieling ◽  
Reinder L. H. Bolhuis ◽  
Egbert J. E. G. Bast
Keyword(s):  
T Lymphocytes ◽  
Hollow Fiber ◽  
Large Scale ◽  
Scale Production ◽  
Hollow Fiber Bioreactor ◽  
Human T Lymphocytes ◽  
Large Scale Production ◽  
Bioreactor Cultures

A novel method for the large-scale production of PG-CNP37, a C-type natriuretic peptide analogue

2013 ◽  
Vol 164 (2) ◽  
pp. 196-201 ◽  
Author(s):  
Shinong Long ◽  
Daniel J. Wendt ◽  
Sean M. Bell ◽  
Timothy W. Taylor ◽  
Jean-Yves Dewavrin ◽  
...  
Keyword(s):  
Natriuretic Peptide ◽  
Large Scale ◽  
Scale Production ◽  
Peptide Analogue ◽  
Large Scale Production ◽  
Novel Method
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