Luminescence Studies of Polyelectrolyte Behavior in Solution. 3. Time-Resolved Fluorescence Anisotropy Measurements of the Conformational Behavior of Poly(methacrylic acid) in Dilute Aqueous Solutions

1994 ◽  
Vol 27 (15) ◽  
pp. 4304-4311 ◽  
Author(s):  
Ian Soutar ◽  
Linda Swanson
Keyword(s):  
Aqueous Solutions ◽  
Methacrylic Acid ◽  
Fluorescence Anisotropy ◽  
Conformational Behavior ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Dilute Aqueous Solutions

Study of polymer chain dynamics in solution by time-resolved spectrofluorometry

1989 ◽  
Vol 54 (11) ◽  
pp. 3011-3024 ◽  
Author(s):  
Vlastimil Fidler ◽  
Stefan Vajda ◽  
Zuzana Limpouchová ◽  
Jiří Dvořák ◽  
Karel Procházka ◽  
...  
Keyword(s):  
Aqueous Solutions ◽  
Conformational Changes ◽  
Methacrylic Acid ◽  
Correlation Time ◽  
General Level ◽  
Polymeric Chain ◽  
Relative Molecular Mass ◽  
Chain Dynamics ◽  
Time Resolved ◽  
Covalently Bonded

The methodology of polarization time-resolved fluorometry and interpretation of its results are outlined at a general level, and the measurement on and use of facilities of the Edinburgh Instruments Model 299T apparatus are discussed in detail. The dynamics of conformational changes in chains of poly(methacrylic acid) containing covalently bonded dansyl labels are studied in aqueous solutions at various pH. It is shown that at pH > 6, the shorter effective rational correlation time τr < 2 ns corresponds to the rotation of the free dansyl label about bonds by which it is attached to the polymeric chain; at pH < 4 the longer effective rational correlation time τr = 20-26 ns corresponds to the rotation of the compact spherical formation constituted by a part of the collapsed polymeric chain in which the label is fixed and whose relative molecular mass is approx. 15 000-20 000.

scholarly journals Time-Resolved Fluorescence Anisotropy and Molecular Dynamics Analysis of a Novel GFP Homo-FRET Dimer

2020 ◽  
Author(s):  
Yurema Teijeiro-Gonzalez ◽  
Alessandro Crnjar ◽  
Andrew J. Beavil ◽  
Rebecca L. Beavil ◽  
Jakub Nedbal ◽  
...  
Keyword(s):  
Molecular Dynamics ◽  
Fluorescence Anisotropy ◽  
Dynamics Analysis ◽  
Time Resolved Fluorescence ◽  
Time Resolved

Nanoparticle metrology standards based on the time-resolved fluorescence anisotropy of silica colloids

2009 ◽  
Vol 20 (2) ◽  
pp. 025310 ◽  
Author(s):  
Kathleen Apperson ◽  
Jan Karolin ◽  
Robert W Martin ◽  
David J S Birch
Keyword(s):  
Fluorescence Anisotropy ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Silica Colloids

Local Chain Dynamics of Adsorbed Polystyrene Studied by Time-Resolved Fluorescence Anisotropy

2001 ◽  
Vol 34 (24) ◽  
pp. 8401-8404 ◽  
Author(s):  
Sangmin Jeon ◽  
Sung Chul Bae ◽  
Steve Granick
Keyword(s):  
Fluorescence Anisotropy ◽  
Time Resolved Fluorescence ◽  
Chain Dynamics ◽  
Time Resolved

scholarly journals Nanosecond Dynamics of Calmodulin and Ribosome-Bound Nascent Chains Studied by Time-Resolved Fluorescence Anisotropy

2014 ◽  
Vol 106 (2) ◽  
pp. 670a-671a
Author(s):  
Daryan Kempe ◽  
Paraskevas Lamprou ◽  
Alexandros Katranidis ◽  
Georg Büldt ◽  
Jörg Fitter
Keyword(s):  
Fluorescence Anisotropy ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Nanosecond Dynamics
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