Identifying Novel Protein Complexes in Cancer Cells Using Epitope-Tagging of Endogenous Human Genes and Affinity-Purification Mass Spectrometry

Jing Song; Yujun Hao; Zhanwen Du; Zhenghe Wang; Rob M. Ewing

doi:10.1021/pr300598t

Identifying Novel Protein Complexes in Cancer Cells Using Epitope-Tagging of Endogenous Human Genes and Affinity-Purification Mass Spectrometry

Journal of Proteome Research ◽

10.1021/pr300598t ◽

2012 ◽

Vol 11 (12) ◽

pp. 5630-5641 ◽

Cited By ~ 16

Author(s):

Jing Song ◽

Yujun Hao ◽

Zhanwen Du ◽

Zhenghe Wang ◽

Rob M. Ewing

Keyword(s):

Mass Spectrometry ◽

Cancer Cells ◽

Protein Complexes ◽

Affinity Purification ◽

Epitope Tagging ◽

Human Genes ◽

Affinity Purification Mass Spectrometry ◽

Novel Protein

Download Full-text

A Tag-Based Affinity Purification Mass Spectrometry Workflow for Systematic Isolation of the Human Mitochondrial Protein Complexes

Mitochondria in Health and in Sickness - Advances in Experimental Medicine and Biology ◽

10.1007/978-981-13-8367-0_6 ◽

2019 ◽

pp. 83-100

Author(s):

Zhuoran Wu ◽

Ramy Malty ◽

Mohamed Taha Moutaoufik ◽

Qingzhou Zhang ◽

Matthew Jessulat ◽

...

Keyword(s):

Mass Spectrometry ◽

Protein Complexes ◽

Affinity Purification ◽

Mitochondrial Protein ◽

Affinity Purification Mass Spectrometry

Download Full-text

Identification of Protein Complexes from Tandem Affinity Purification/Mass Spectrometry Data via Biased Random Walk

IEEE/ACM Transactions on Computational Biology and Bioinformatics ◽

10.1109/tcbb.2014.2352616 ◽

2015 ◽

Vol 12 (2) ◽

pp. 455-466 ◽

Cited By ~ 3

Author(s):

Bingjing Cai ◽

Haiying Wang ◽

Huiru Zheng ◽

Hui Wang

Keyword(s):

Mass Spectrometry ◽

Random Walk ◽

Protein Complexes ◽

Affinity Purification ◽

Tandem Affinity Purification ◽

Mass Spectrometry Data ◽

Biased Random Walk ◽

Affinity Purification Mass Spectrometry

Download Full-text

Incorporating semantic similarity into clustering process for identifying protein complexes from Affinity Purification/Mass Spectrometry data

2012 IEEE International Conference on Bioinformatics and Biomedicine ◽

10.1109/bibm.2012.6392718 ◽

2012 ◽

Author(s):

Bingjing Cai ◽

Haiying Wang ◽

Huiru Zheng ◽

Hui Wang

Keyword(s):

Mass Spectrometry ◽

Semantic Similarity ◽

Protein Complexes ◽

Affinity Purification ◽

Mass Spectrometry Data ◽

Affinity Purification Mass Spectrometry

Download Full-text

Rapid isolation of aphid-virus protein complexes from viruliferous insects using affinity purification-mass spectrometry

10.1603/ice.2016.107795 ◽

2016 ◽

Author(s):

Stacy L. DeBlasio

Keyword(s):

Mass Spectrometry ◽

Protein Complexes ◽

Affinity Purification ◽

Virus Protein ◽

Rapid Isolation ◽

Affinity Purification Mass Spectrometry

Download Full-text

Analyzing protein complexes inDrosophila with tandem affinity purification-mass spectrometry

Developmental Dynamics ◽

10.1002/dvdy.20272 ◽

2005 ◽

Vol 232 (3) ◽

pp. 827-834 ◽

Cited By ~ 76

Author(s):

Alexey Veraksa ◽

Andreas Bauer ◽

Spyros Artavanis-Tsakonas

Keyword(s):

Mass Spectrometry ◽

Protein Complexes ◽

Affinity Purification ◽

Tandem Affinity Purification ◽

Affinity Purification Mass Spectrometry

Download Full-text

Detection of protein complexes from affinity purification/mass spectrometry data

BMC Systems Biology ◽

10.1186/1752-0509-6-s3-s4 ◽

2012 ◽

Vol 6 (Suppl 3) ◽

pp. S4 ◽

Cited By ~ 5

Author(s):

Bingjing Cai ◽

Haiying Wang ◽

Huiru Zheng ◽

Hui Wang

Keyword(s):

Mass Spectrometry ◽

Protein Complexes ◽

Affinity Purification ◽

Mass Spectrometry Data ◽

Affinity Purification Mass Spectrometry

Download Full-text

Faculty Opinions recommendation of Sortase A mediated site-specific immobilization for identification of protein interactions in affinity purification-mass spectrometry experiments.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.725280481.793522616 ◽

2016 ◽

Author(s):

Morten Meldal ◽

Sanne Schoffelen

Keyword(s):

Mass Spectrometry ◽

Protein Interactions ◽

Affinity Purification ◽

Sortase A ◽

Site Specific ◽

Affinity Purification Mass Spectrometry

Download Full-text

SAINT: probabilistic scoring of affinity purification–mass spectrometry data

Nature Methods ◽

10.1038/nmeth.1541 ◽

2010 ◽

Vol 8 (1) ◽

pp. 70-73 ◽

Cited By ~ 414

Author(s):

Hyungwon Choi ◽

Brett Larsen ◽

Zhen-Yuan Lin ◽

Ashton Breitkreutz ◽

Dattatreya Mellacheruvu ◽

...

Keyword(s):

Mass Spectrometry ◽

Affinity Purification ◽

Mass Spectrometry Data ◽

Affinity Purification Mass Spectrometry

Download Full-text

Delineating WWOX Protein Interactome by Tandem Affinity Purification-Mass Spectrometry: Identification of Top Interactors and Key Metabolic Pathways Involved

Frontiers in Oncology ◽

10.3389/fonc.2018.00591 ◽

2018 ◽

Vol 8 ◽

Cited By ~ 7

Author(s):

Tabish Hussain ◽

Jaeho Lee ◽

Martin C. Abba ◽

Junjie Chen ◽

C. Marcelo Aldaz

Keyword(s):

Mass Spectrometry ◽

Metabolic Pathways ◽

Affinity Purification ◽

Tandem Affinity Purification ◽

Protein Interactome ◽

Mass Spectrometry Identification ◽

Affinity Purification Mass Spectrometry

Download Full-text

Reinvestigation of Aminoacyl-TRNA Synthetase Core Complex by Affinity Purification-Mass Spectrometry Reveals TARSL2 as a Potential Member of the Complex

PLoS ONE ◽

10.1371/journal.pone.0081734 ◽

2013 ◽

Vol 8 (12) ◽

pp. e81734 ◽

Cited By ~ 11

Author(s):

Kyutae Kim ◽

Seong-Jun Park ◽

Seungjin Na ◽

Jun Seok Kim ◽

Hyungwon Choi ◽

...

Keyword(s):

Mass Spectrometry ◽

Affinity Purification ◽

Trna Synthetase ◽

Aminoacyl Trna Synthetase ◽

Core Complex ◽

Affinity Purification Mass Spectrometry ◽

Potential Member

Download Full-text