Synthetic Approach to Gain Insight into Antigenic Determinants of Cephalosporins: In Vitro Studies of Chemical Structure−IgE Molecular Recognition Relationships

2011 ◽  
Vol 24 (5) ◽  
pp. 706-717 ◽  
Author(s):  
Maria Isabel Montañez ◽  
Cristobalina Mayorga ◽  
Maria Jose Torres ◽  
Adriana Ariza ◽  
Miguel Blanca ◽  
...  
Keyword(s):  
Molecular Recognition ◽  
Chemical Structure ◽  
In Vitro Studies ◽  
Antigenic Determinants ◽  
Synthetic Approach ◽  
Insight Into

scholarly journals In vitro studies provide insight into effects of Dicer-2 helicase mutations in Drosophila melanogaster

RNA ◽  
2020 ◽  
Vol 26 (12) ◽  
pp. 1847-1861 ◽  
Author(s):  
Helen M. Donelick ◽  
Loïc Talide ◽  
Matthieu Bellet ◽  
P. Joseph Aruscavage ◽  
Emilie Lauret ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
In Vitro Studies ◽  
Insight Into

scholarly journals Structure and Dynamics of GPCRs in Lipid Membranes: Physical Principles and Experimental Approaches

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4729
Author(s):  
Andrew J. Y. Jones ◽  
Florian Gabriel ◽  
Aditi Tandale ◽  
Daniel Nietlispach
Keyword(s):  
Lipid Bilayer ◽  
Lipid Membranes ◽  
In Vitro Studies ◽  
Specific Effects ◽  
Biophysical Studies ◽  
Experimental Approaches ◽  
Specific Lipid ◽  
Physical Principles ◽  
Insight Into

Over the past decade, the vast amount of information generated through structural and biophysical studies of GPCRs has provided unprecedented mechanistic insight into the complex signalling behaviour of these receptors. With this recent information surge, it has also become increasingly apparent that in order to reproduce the various effects that lipids and membranes exert on the biological function for these allosteric receptors, in vitro studies of GPCRs need to be conducted under conditions that adequately approximate the native lipid bilayer environment. In the first part of this review, we assess some of the more general effects that a membrane environment exerts on lipid bilayer-embedded proteins such as GPCRs. This is then followed by the consideration of more specific effects, including stoichiometric interactions with specific lipid subtypes. In the final section, we survey a range of different membrane mimetics that are currently used for in vitro studies, with a focus on NMR applications.

In vitro - studies with antler bone cells: Structure forming capacity, osteocalcin production and influence of sex steroids

2006 ◽  
Vol 15 (04) ◽  
pp. 245-257 ◽  
Author(s):  
H. J. Rolf ◽  
K. G. Wiese ◽  
H. Siggelkow ◽  
H. Schliephake ◽  
G. A. Bubernik
Keyword(s):  
Sex Steroids ◽  
Bone Cells ◽  
In Vitro Studies ◽  
Osteocalcin Production

In Vitro Studies on the Mechanism of the Antifibrino-lytic Action of E-Aminocaproic Acid (EACA)

1968 ◽  
Vol 19 (03/04) ◽  
pp. 584-592 ◽  
Author(s):  
Hanna Lukasiewicz ◽  
S Niewiarowski
Keyword(s):  
Aminocaproic Acid ◽  
Test Tube ◽  
In Vitro Studies ◽  
Lytic Action ◽  
Human Plasminogen ◽  
Experimental Findings ◽  
Fibrin Clots

Summary and Conclusion1. It has been found that EACA does not inhibit activation of human plasminogen into plasmin by SK and UK in a concentration of 5 × 10–2 M. The activation of bovine plasminogen by SK and UK is inhibited by this concentration of EACA but not by a lower one.2. EACA in concentrations of 1,5 × 10–1 – 10–4 M does not inhibit casein proteolysis by plasmin. The proteolysis of fibrinogen and fibrin measured by the release of TCA soluble tyrosine is inhibited by EACA in concentrations of 1,5 × 10–1 – 10–2 M.3. The lysis of non-stabilized clots by plasmin measured in a test tube was inhibited by an EACA concentration of 5 × 10–3 – 5 × 10–4 M. The lysis of stabilized clots by plasmin was inhibited by an EACA concentration of 10–5 M.4. On the basis of experimental findings and data given in literature the authors postulate that the mechanism of the antifibrinolytic effects of EACA consists mainly in a modification of plasmin action on fibrin. These effects are dependent on the structure of the fibrin clots.

The Venom of the Boomslang (Dispholidus Typus): In Vivo and in Vitro Studies

1969 ◽  
Vol 21 (02) ◽  
pp. 234-244 ◽  
Author(s):  
N Mackay ◽  
J.C Ferguson ◽  
Antonia Bagshawe ◽  
A.T.T Forrester ◽  
G.P Mcnicol
Keyword(s):  
In Vitro Studies

SummaryAn account is given of the effects of boomslang venom in man. Evidence was found of a fibrinolytic state apparently secondary to the coagulant action of the venom. These features rapidly responded to the administration of specific antivenom. In vitro studies, using a homogenate of boomslang parotids, confirmed the coagulant properties of the venom and showed them to be of much greater potency than the proteolytic actions.

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