Two-State Folding of Lysozyme Versus Multiple-State Folding of α-Lactalbumin Illustrated by the Technique of Disulfide Scrambling

Abstract. The pulmonary arteries are affected by a variety of congenital and acquired abnormalities. Multiple state-of-the art imaging modalities are available to evaluate these pulmonary arterial abnormalities, including computed tomography (CT), magnetic resonance imaging (MRI), echocardiography, nuclear medicine imaging and catheter pulmonary angiography. In part one of this two-part series on state-of-the art pulmonary arterial imaging, we review these imaging modalities, focusing particularly on CT and MRI. We also review the utility of these imaging modalities in the evaluation of pulmonary thromboembolism.

Download Full-text

Review on the Application of Mixed-mode Chromatography for Separation of Structure Isoforms

Current Protein and Peptide Science ◽

10.2174/1389203718666171009111033 ◽

2018 ◽

Vol 20 (1) ◽

pp. 56-60 ◽

Cited By ~ 3

Author(s):

Tsutomu Arakawa

Keyword(s):

Ion Exchange ◽

Mixed Mode ◽

Ion Exchange Chromatography ◽

Exchange Chromatography ◽

Charged State ◽

Partial Structure ◽

Reverse Phase ◽

Oligomer Formation ◽

Structure Rearrangement ◽

Disulfide Scrambling

Proteins often generate structure isoforms naturally or artificially due to, for example, different glycosylation, disulfide scrambling, partial structure rearrangement, oligomer formation or chemical modification. The isoform formations are normally accompanied by alterations in charged state or hydrophobicity. Thus, isoforms can be fractionated by reverse-phase, hydrophobic interaction or ion exchange chromatography. We have applied mixed-mode chromatography for fractionation of isoforms for several model proteins and observed that cation exchange Capto MMC and anion exchange Capto adhere columns are effective in separating conformational isoforms and self-associated oligomers.

Download Full-text

Navigating the Complex Maze of Ethics CPE

Accounting and the Public Interest ◽

10.2308/api.2010.10.1.88 ◽

2010 ◽

Vol 10 (1) ◽

pp. 88-104 ◽

Cited By ~ 3

Author(s):

Howard O. Rockness ◽

Joanne W. Rockness

Keyword(s):

Detailed Analysis ◽

Ethics Education ◽

State Level ◽

Course Content ◽

Complex Maze ◽

Education Literature ◽

Current State ◽

Multiple State ◽

Ethics Courses ◽

Overall Effectiveness

ABSTRACT: This paper evaluates the current state of ethics CPE requirements for the CPA profession in the context of the ethics literature in philosophy, business, and accounting and documents the development of state-level ethics requirements for CPAs. It presents a detailed analysis of ethics CPE requirements by state including hours required, frequency required, acceptance of ethics CPE across state lines, and course content. The paper then proposes changes in the content of CPE-required ethics courses consistent with the ethics education literature and to reduce the complexity of meeting multiple state requirements. The recommendations would improve the overall effectiveness of ethics CPE for CPAs.

Download Full-text

The CDC Field Epidemiology Manual

10.1093/oso/9780190933692.001.0001 ◽

2019 ◽

Cited By ~ 3

Keyword(s):

Disease Outbreaks ◽

Incident Management ◽

The Public ◽

Field Epidemiology ◽

Field Investigations ◽

Toxic Agents ◽

Core Elements ◽

Multiple State ◽

Prompt Action ◽

Collection And Management

The CDC Field Epidemiology Manual is the definitive resource for the most up-to-date guidance for epidemiologists and other experts conducting field investigations to address acute public health concerns that require prompt action. This latest edition (an update of the 3rd edition of the popular book Field Epidemiology, edited by Dr. Michael Gregg) offers practical advice to guide investigators through the core elements of field investigations, beginning with initiating operations and ending with developing interventions and communicating findings to the public. The manual also provides special considerations to address challenges that often arise during field investigations, such as addressing legal issues, working with multiple state and federal agencies, navigating a multinational outbreak investigation, and working within an incident management structure. The manual includes updated information on using new tools for field investigations, such as the latest technologies for data collection and management and incorporating data from geographic information systems (GIS). Finally, the manual includes tips for investigations in a wide variety of settings, including healthcare and community congregate settings, and different types of outbreaks, including acute enteric disease outbreaks, those suspected to be related to intentional use of biologic and toxic agents, and outbreaks of suicide, violence, and other forms of injury. The manual is written primarily for epidemiologists who will be conducting field investigations in local, state, federal, or international settings. However, others who contribute to field investigations (e.g., laboratory scientists, lawyers, experts in public policy and communications) will also find the book to be an excellent source of information. The manual is written in an easily readable format, including boxes and bulleted points, to provide greater utility for investigators in the field.

Download Full-text