The appearance of single-ion channels in unmodified lipid bilayer membranes at the phase transition temperature

Nature ◽  
1980 ◽  
Vol 283 (5747) ◽  
pp. 585-586 ◽  
Author(s):  
V. F. Antonov ◽  
V. V. Petrov ◽  
A. A. Molnar ◽  
D. A. Predvoditelev ◽  
A. S. Ivanov
Keyword(s):  
Phase Transition ◽  
Transition Temperature ◽  
Ion Channels ◽  
Lipid Bilayer ◽  
Phase Transition Temperature ◽  
Lipid Bilayer Membranes ◽  
Bilayer Membranes

scholarly journals Variation of thermal conductivity of DPPC lipid bilayer membranes around the phase transition temperature

2017 ◽  
Vol 14 (130) ◽  
pp. 20170127 ◽  
Author(s):  
Sina Youssefian ◽  
Nima Rahbar ◽  
Christopher R. Lambert ◽  
Steven Van Dessel
Keyword(s):  
Phase Transition ◽  
Thermal Conductivity ◽  
Thermal Properties ◽  
Transition Temperature ◽  
Lipid Bilayer ◽  
Lipid Bilayers ◽  
Phase Transition Temperature ◽  
Temperature Gradients ◽  
Phase Behaviour ◽  
Gel Phase

Given their amphiphilic nature and chemical structure, phospholipids exhibit a strong thermotropic and lyotropic phase behaviour in an aqueous environment. Around the phase transition temperature, phospholipids transform from a gel-like state to a fluid crystalline structure. In this transition, many key characteristics of the lipid bilayers such as structure and thermal properties alter. In this study, we employed atomistic simulation techniques to study the structure and underlying mechanisms of heat transfer in dipalmitoylphosphatidylcholine (DPPC) lipid bilayers around the fluid–gel phase transformation. To investigate this phenomenon, we performed non-equilibrium molecular dynamics simulations for a range of different temperature gradients. The results show that the thermal properties of the DPPC bilayer are highly dependent on the temperature gradient. Higher temperature gradients cause an increase in the thermal conductivity of the DPPC lipid bilayer. We also found that the thermal conductivity of DPPC is lowest at the transition temperature whereby one lipid leaflet is in the gel phase and the other is in the liquid crystalline phase. This is essentially related to a growth in thermal resistance between the two leaflets of lipid at the transition temperature. These results provide significant new insights into developing new thermal insulation for engineering applications.

Effect of the Phase Transition on the Photochemical Reactions in Lipid Bilayer Membranes

1981 ◽  
Vol 68 (1) ◽  
pp. 69-78
Author(s):  
Kazue Kurihara ◽  
Kaoru Onuki ◽  
Yoshinori Toyoshima ◽  
Mitsunori Sukigara
Keyword(s):  
Phase Transition ◽  
Lipid Bilayer ◽  
Photochemical Reactions ◽  
Lipid Bilayer Membranes ◽  
Bilayer Membranes

Ligand partitioning into lipid bilayer membranes under high pressure: Implication of variation in phase-transition temperatures

2017 ◽  
Vol 209 ◽  
pp. 9-18 ◽  
Author(s):  
Hitoshi Matsuki ◽  
Kentaro Kato ◽  
Hirotsugu Okamoto ◽  
Shuntaro Yoshida ◽  
Masaki Goto ◽  
...  
Keyword(s):  
Phase Transition ◽  
High Pressure ◽  
Lipid Bilayer ◽  
Transition Temperatures ◽  
Lipid Bilayer Membranes ◽  
Bilayer Membranes ◽  
Phase Transition Temperatures

The Effect of the Lipid Bilayer State on Fluorescence Intensity of Fluorescein-PE in a Saturated Lipid Bilayer

2000 ◽  
Vol 55 (5-6) ◽  
pp. 418-424 ◽  
Author(s):  
Marek Langner ◽  
Hanna Pruchnik ◽  
Krystian Kubica
Keyword(s):  
Phase Transition ◽  
Dielectric Constant ◽  
Transition Temperature ◽  
Lipid Bilayer ◽  
Phase Transition Temperature ◽  
Conformational Changes ◽  
Fluorescence Intensity ◽  
Electrostatic Potential ◽  
Salt Concentration ◽  
Membrane Interface

Fluorescein-PE is a fluorescence probe that is used as a membrane label or a sensor of surface associated processes. Fluorescein-PE fluorescence intensity depends not only on bulk pH, but also on the local electrostatic potential, which affects the local membrane interface proton concentration. The pH sensitivity and hydrophilic character of the fluorescein moiety was used to detect conformational changes at the lipid bilayer surface. When located in the dipalmitoylphosphatidylcholine (DPPC) bilayer, probe fluorescence depends on conformational changes that occur during phase transitions. Relative fluorescence intensity changes more at pretransition than at the main phase transition temperature, indicating that interface conformation affects the condition in the vicinity of the membrane. Local electrostatic potential depends on surface charge density, the local dielectric constant, salt concentration and water organisation. Initial increase in fluorescence intensity at temperatures preceding that of pretransition can be explained by the decreased value of the dielectric constant in the lipid polar headgroups region related in turn to decreased water organisation within the membrane interface. The abrupt decrease in fluorescence intensity at temperatures between 25 °C and 35 °C (DPPC pretransition) is likely to be caused by an increased value of the electrostatic potential, induced by an elevated value of the dielectric constant within the phosphate group region. Further increase in the fluorescence intensity at temperatures above that of the gel-liquid phase transition correlates with the calculated decreased surface electrostatic potential. Above the main phase transition temperature, fluorescence intensity increase at a salt concentration of 140 m M is larger than with 14 m M . This results from a sharp decline of the electrostatic potential induced by the phosphocholine dipole as a function of distance from the membrane surface.

Synthetic peptides form ion channels in artificial lipid bilayer membranes

Science ◽  
1977 ◽  
Vol 196 (4296) ◽  
pp. 1341-1342 ◽  
Author(s):  
S. Kennedy ◽  
R. Roeske ◽  
A. Freeman ◽  
A. Watanabe ◽  
H. Besche
Keyword(s):  
Ion Channels ◽  
Lipid Bilayer ◽  
Synthetic Peptides ◽  
Lipid Bilayer Membranes ◽  
Bilayer Membranes

scholarly journals A simple method for the determination of the pore radius of ion channels in planar lipid bilayer membranes

1992 ◽  
Vol 105 (1-3) ◽  
pp. 93-100 ◽  
Author(s):  
O.V. Krasilnikov ◽  
R.Z. Sabirov ◽  
V.I. Ternovsky ◽  
P.G. Merzliak ◽  
J.N. Muratkhodjaev
Keyword(s):  
Ion Channels ◽  
Lipid Bilayer ◽  
Pore Radius ◽  
Planar Lipid Bilayer ◽  
Lipid Bilayer Membranes ◽  
Simple Method ◽  
Bilayer Membranes
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