scholarly journals High efficiency transduction of human VEGF165 into human skeletal myoblasts: in vitro studies

2003 ◽  
Vol 35 (5) ◽  
pp. 412-420 ◽  
Author(s):  
Lei Ye ◽  
Husnain Kh Haider ◽  
Shujia Jiang ◽  
Ruowen Ge ◽  
Peter K Law ◽  
...  
Keyword(s):  
High Efficiency ◽  
In Vitro Studies ◽  
Skeletal Myoblasts

scholarly journals Human epicardium-derived cells fuse with high efficiency with skeletal myotubes and differentiate toward the skeletal muscle phenotype: a comparison study with stromal and endothelial cells

2011 ◽  
Vol 22 (5) ◽  
pp. 581-592 ◽  
Author(s):  
Antonietta Gentile ◽  
Gabriele Toietta ◽  
Vincenzo Pazzano ◽  
Vasileios D. Tsiopoulos ◽  
Ada Francesca Giglio ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
High Efficiency ◽  
Low Frequency ◽  
Mdx Mice ◽  
Skeletal Myoblasts ◽  
Primary Myoblasts ◽  
Multipotent Cells ◽  
Nonmuscle Cells ◽  
Cell Adhesion Molecule 1

Recent studies have underscored a role for the epicardium as a source of multipotent cells. Here, we investigate the myogenic potential of adult human epicardium-derived cells (EPDCs) and analyze their ability to undergo skeletal myogenesis when cultured with differentiating primary myoblasts. Results are compared to those obtained with mesenchymal stromal cells (MSCs) and with endothelial cells, another mesodermal derivative. We demonstrate that EPDCs spontaneously fuse with pre-existing myotubes with an efficiency that is significantly higher than that of other cells. Although at a low frequency, endothelial cells may also contribute to myotube formation. In all cases analyzed, after entering the myotube, nonmuscle nuclei are reprogrammed to express muscle-specific genes. The fusion competence of nonmyogenic cells in vitro parallels their ability to reconstitute dystrophin expression in mdx mice. We additionally show that vascular cell adhesion molecule 1 (VCAM1) expression levels of nonmuscle cells are modulated by soluble factors secreted by skeletal myoblasts and that VCAM1 function is required for fusion to occur. Finally, treatment with interleukin (IL)-4 or IL-13, two cytokines released by differentiating myotubes, increases VCAM1 expression and enhances the rate of fusion of EPDCs and MSCs, but not that of endothelial cells.

In vitro - studies with antler bone cells: Structure forming capacity, osteocalcin production and influence of sex steroids

2006 ◽  
Vol 15 (04) ◽  
pp. 245-257 ◽  
Author(s):  
H. J. Rolf ◽  
K. G. Wiese ◽  
H. Siggelkow ◽  
H. Schliephake ◽  
G. A. Bubernik
Keyword(s):  
Sex Steroids ◽  
Bone Cells ◽  
In Vitro Studies ◽  
Osteocalcin Production

Selective Glucocorticoid Receptor Agonists for Treatment of Inflammatory Bowel Disease – in vitro studies

2011 ◽  
Vol 49 (10) ◽  
Author(s):  
Kerstin Reuter ◽  
Stefan Loitsch ◽  
Axel Dignass ◽  
Jürgen Stein
Keyword(s):  
Inflammatory Bowel Disease ◽  
Glucocorticoid Receptor ◽  
Bowel Disease ◽  
In Vitro Studies ◽  
Receptor Agonists ◽  
Inflammatory Bowel

Biological and physico-chemical characterization of ultra high diluted Hydrastis canadensis and in vitro studies on mammalian cells

2017 ◽  
Vol 262 (02) ◽  
pp. 2-76
Author(s):  
N Chandrakar ◽  
MK Temgire ◽  
SG Kane ◽  
AK Suresh ◽  
J Bellare
Keyword(s):  
Mammalian Cells ◽  
Chemical Characterization ◽  
In Vitro Studies ◽  
Hydrastis Canadensis ◽  
Physico Chemical

In Vitro Studies on the Mechanism of the Antifibrino-lytic Action of E-Aminocaproic Acid (EACA)

1968 ◽  
Vol 19 (03/04) ◽  
pp. 584-592 ◽  
Author(s):  
Hanna Lukasiewicz ◽  
S Niewiarowski
Keyword(s):  
Aminocaproic Acid ◽  
Test Tube ◽  
In Vitro Studies ◽  
Lytic Action ◽  
Human Plasminogen ◽  
Experimental Findings ◽  
Fibrin Clots

Summary and Conclusion1. It has been found that EACA does not inhibit activation of human plasminogen into plasmin by SK and UK in a concentration of 5 × 10–2 M. The activation of bovine plasminogen by SK and UK is inhibited by this concentration of EACA but not by a lower one.2. EACA in concentrations of 1,5 × 10–1 – 10–4 M does not inhibit casein proteolysis by plasmin. The proteolysis of fibrinogen and fibrin measured by the release of TCA soluble tyrosine is inhibited by EACA in concentrations of 1,5 × 10–1 – 10–2 M.3. The lysis of non-stabilized clots by plasmin measured in a test tube was inhibited by an EACA concentration of 5 × 10–3 – 5 × 10–4 M. The lysis of stabilized clots by plasmin was inhibited by an EACA concentration of 10–5 M.4. On the basis of experimental findings and data given in literature the authors postulate that the mechanism of the antifibrinolytic effects of EACA consists mainly in a modification of plasmin action on fibrin. These effects are dependent on the structure of the fibrin clots.

The Venom of the Boomslang (Dispholidus Typus): In Vivo and in Vitro Studies

1969 ◽  
Vol 21 (02) ◽  
pp. 234-244 ◽  
Author(s):  
N Mackay ◽  
J.C Ferguson ◽  
Antonia Bagshawe ◽  
A.T.T Forrester ◽  
G.P Mcnicol
Keyword(s):  
In Vitro Studies

SummaryAn account is given of the effects of boomslang venom in man. Evidence was found of a fibrinolytic state apparently secondary to the coagulant action of the venom. These features rapidly responded to the administration of specific antivenom. In vitro studies, using a homogenate of boomslang parotids, confirmed the coagulant properties of the venom and showed them to be of much greater potency than the proteolytic actions.

Effect of X-irradiation on gene expression of rat liver chemokines: in vivo and in vitro studies

2008 ◽  
Vol 46 (01) ◽  
Author(s):  
F Moriconi ◽  
H Christiansen ◽  
H Christiansen ◽  
N Sheikh ◽  
J Dudas ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rat Liver ◽  
In Vitro Studies ◽  
X Irradiation

IN VITRO STUDIES ON LIPOLYSIS IN HUMAN ADIPOSE TISSUE

1969 ◽  
Vol 61 (1_Suppl) ◽  
pp. S156
Author(s):  
Suad Efendić ◽  
Peter Amer ◽  
Jan Östman
Keyword(s):  
Adipose Tissue ◽  
Human Adipose Tissue ◽  
In Vitro Studies
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