scholarly journals Mass spectrometry of intact membrane protein complexes

2013 ◽  
Vol 8 (4) ◽  
pp. 639-651 ◽  
Author(s):  
Arthur Laganowsky ◽  
Eamonn Reading ◽  
Jonathan T S Hopper ◽  
Carol V Robinson
Keyword(s):  
Mass Spectrometry ◽  
Membrane Protein ◽  
Protein Complexes ◽  
Intact Membrane ◽  
Membrane Protein Complexes

scholarly journals Controlling release, unfolding and dissociation of membrane protein complexes in the gas phase through collisional cooling

2015 ◽  
Vol 51 (85) ◽  
pp. 15582-15584 ◽  
Author(s):  
Michael Landreh ◽  
Idlir Liko ◽  
Povilas Uzdavinys ◽  
Mathieu Coincon ◽  
Jonathan T. S. Hopper ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Membrane Protein ◽  
Gas Phase ◽  
Protein Complexes ◽  
Intact Membrane ◽  
Collisional Cooling ◽  
Membrane Protein Complexes ◽  
Detergent Micelles

Reduced collisional cooling releases intact membrane protein complexes from detergent micelles for unfolding and dissociation studies by mass spectrometry.

scholarly journals Top-down mass spectrometry of intact membrane protein complexes reveals oligomeric state and sequence information in a single experiment

2015 ◽  
Vol 24 (8) ◽  
pp. 1292-1300 ◽  
Author(s):  
Albert Konijnenberg ◽  
Ludovic Bannwarth ◽  
Duygu Yilmaz ◽  
Armağan Koçer ◽  
Catherine Venien-Bryan ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Membrane Protein ◽  
Protein Complexes ◽  
Sequence Information ◽  
Oligomeric State ◽  
Top Down ◽  
Single Experiment ◽  
Intact Membrane ◽  
Membrane Protein Complexes ◽  
Top Down Mass Spectrometry

scholarly journals Charge Reduction Stabilizes Intact Membrane Protein Complexes for Mass Spectrometry

2014 ◽  
Vol 136 (49) ◽  
pp. 17010-17012 ◽  
Author(s):  
Shahid Mehmood ◽  
Julien Marcoux ◽  
Jonathan T. S. Hopper ◽  
Timothy M. Allison ◽  
Idlir Liko ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Membrane Protein ◽  
Protein Complexes ◽  
Charge Reduction ◽  
Intact Membrane ◽  
Membrane Protein Complexes

Ion Mobility Mass Spectrometry of Two Tetrameric Membrane Protein Complexes Reveals Compact Structures and Differences in Stability and Packing

2010 ◽  
Vol 132 (44) ◽  
pp. 15468-15470 ◽  
Author(s):  
Sheila C. Wang ◽  
Argyris Politis ◽  
Natalie Di Bartolo ◽  
Vassiliy N. Bavro ◽  
Stephen J. Tucker ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Membrane Protein ◽  
Ion Mobility ◽  
Protein Complexes ◽  
Ion Mobility Mass Spectrometry ◽  
Membrane Protein Complexes

scholarly journals A Novel Chloroplast Super-Complex Consisting of the ATP Synthase and Photosystem I Reaction Center

2019 ◽  
Author(s):  
Satarupa Bhaduri ◽  
Sandeep K Singh ◽  
Whitaker Cohn ◽  
S. Saif Hasan ◽  
Julian P. Whitelegge ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Membrane Protein ◽  
Reaction Center ◽  
Atp Synthase ◽  
Photosystem I ◽  
Protein Complexes ◽  
Thylakoid Membranes ◽  
Native Page ◽  
Membrane Protein Complexes ◽  
Reaction Center Complexes

AbstractSeveral ‘super-complexes’ of individual hetero-oligomeric membrane protein complexes, whose function is to facilitate intra-membrane electron and proton transfer and harvesting of light energy, have been previously characterized in the mitochondrial cristae and chloroplast thylakoid membranes. The latter membrane is reported here to also be the location of an intra-membrane super-complex which is dominated by the ATP-synthase and photosystem I (PSI) reaction-center complexes, defined by mass spectrometry, clear-native PAGE and Western Blot analyses. This is the first documented presence of ATP synthase in a super-complex with the PSI reaction-center located in the non-appressed stromal domain of the thylakoid membrane.

scholarly journals Mass spectrometry of membrane protein complexes

2019 ◽  
Vol 400 (7) ◽  
pp. 813-829 ◽  
Author(s):  
Julian Bender ◽  
Carla Schmidt
Keyword(s):  
Mass Spectrometry ◽  
Membrane Proteins ◽  
Membrane Protein ◽  
Protein Complexes ◽  
Three Dimensional ◽  
Membrane Protein Complexes ◽  
Hydrophobic Nature ◽  
Alternative Approaches ◽  
Lipid Environment ◽  
And Function

Abstract Membrane proteins are key players in the cell. Due to their hydrophobic nature they require solubilising agents such as detergents or membrane mimetics during purification and, consequently, are challenging targets in structural biology. In addition, their natural lipid environment is crucial for their structure and function further hampering their analysis. Alternative approaches are therefore required when the analysis by conventional techniques proves difficult. In this review, we highlight the broad application of mass spectrometry (MS) for the characterisation of membrane proteins and their interactions with lipids. We show that MS unambiguously identifies the protein and lipid components of membrane protein complexes, unravels their three-dimensional arrangements and further provides clues of protein-lipid interactions.

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