scholarly journals Measurement of drug-target engagement in live cells by two-photon fluorescence anisotropy imaging

2017 ◽  
Vol 12 (7) ◽  
pp. 1472-1497 ◽  
Author(s):  
Claudio Vinegoni ◽  
Paolo Fumene Feruglio ◽  
Christian Brand ◽  
Sungon Lee ◽  
Antoinette E Nibbs ◽  
...  
Keyword(s):  
Fluorescence Anisotropy ◽  
Drug Target ◽  
Live Cells ◽  
Target Engagement ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Open-Chain Crown-Ether-Derived Two-Photon Fluorescence Probe for Real-Time Dynamic Biopsy of Mercury Ions

2017 ◽  
Vol 70 (6) ◽  
pp. 705
Author(s):  
Chibao Huang ◽  
Daohai Zhang ◽  
Junle Qu ◽  
Xiaonan Liu ◽  
Guanglian Zhao ◽  
...  
Keyword(s):  
Water Solubility ◽  
Fluorescence Probe ◽  
Stokes Shift ◽  
Cell Permeability ◽  
Photon Absorption ◽  
Live Cells ◽  
Open Chain ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

A novel two-photon fluorescence probe for Hg2+ derived from bis(styryl)terephthalonitrile, as a two-photon fluorophore, and bis[2-(2-hydroxyethyl sulfanyl) ethyl]amino group (ionophore), as a novel Hg2+ ligand, was developed. The probe possesses small molecule size, large two-photon absorption cross-section (1067 GM) in H2O, non-cytotoxic effect, long wavelength emission at 588 nm, large Stokes shift (121 nm), excellent photostability, high water solubility, good cell permeability, and pH insensitivity in the biologically relevant range. The probe can selectively detect Hg2+ ions in live cells and living tissues without interference from other metal ions and the membrane-bound probes, and its quenching constant is 8.73 × 105 M–1.

Two-photon fluorescence anisotropy imaging

2006 ◽  
Author(s):  
Wei Li ◽  
Yi Wang ◽  
Hanrong Shao ◽  
Yonghong He ◽  
Hui Ma
Keyword(s):  
Fluorescence Anisotropy ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Two-Photon Fluorescence Anisotropy Imaging to Elucidate the Dynamics and the Stability of Immobilized Proteins

2016 ◽  
Vol 120 (3) ◽  
pp. 485-491 ◽  
Author(s):  
Alejandro H. Orrego ◽  
Carolina García ◽  
José M. Mancheño ◽  
Jose M. Guisán ◽  
M. Pilar Lillo ◽  
...  
Keyword(s):  
Fluorescence Anisotropy ◽  
Two Photon ◽  
Immobilized Proteins ◽  
The Stability ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

A two-photon off-on fluorescence probe for imaging thiols in live cells and tissues

2016 ◽  
Vol 15 (3) ◽  
pp. 412-419 ◽  
Author(s):  
Xinyue Zhu ◽  
Yan Li ◽  
Wenyan Zan ◽  
Jianjian Zhang ◽  
Zhenjie Chen ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Fluorescence Probe ◽  
Live Cells ◽  
Imaging Probe ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

An effective off-on two-photon fluorescence imaging probe for thiols was developed and successfully applied to image thiols both in live cells and tissues.

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