scholarly journals Laser solid-phase synthesis of single-atom catalysts

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Yudong Peng ◽  
Jianyun Cao ◽  
Yang Sha ◽  
Wenji Yang ◽  
Lin Li ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Solid Phase ◽  
Catalytic Performance ◽  
Laser Wavelength ◽  
Single Atom ◽  
High Yield ◽  
Phase Synthesis ◽  
Freeze Dried ◽  
Catalytic Sites ◽  
Scanning Irradiation

AbstractSingle-atom catalysts (SACs) with atomically dispersed catalytic sites have shown outstanding catalytic performance in a variety of reactions. However, the development of facile and high-yield techniques for the fabrication of SACs remains challenging. In this paper, we report a laser-induced solid-phase strategy for the synthesis of Pt SACs on graphene support. Simply by rapid laser scanning/irradiation of a freeze-dried electrochemical graphene oxide (EGO) film loaded with chloroplatinic acid (H2PtCl6), we enabled simultaneous pyrolysis of H2PtCl6 into SACs and reduction/graphitization of EGO into graphene. The rapid freezing of EGO hydrogel film infused with H2PtCl6 solution in liquid nitrogen and the subsequent ice sublimation by freeze-drying were essential to achieve the atomically dispersed Pt. Nanosecond pulsed infrared (IR; 1064 nm) and picosecond pulsed ultraviolet (UV; 355 nm) lasers were used to investigate the effects of laser wavelength and pulse duration on the SACs formation mechanism. The atomically dispersed Pt on graphene support exhibited a small overpotential of −42.3 mV at −10 mA cm−2 for hydrogen evolution reaction and a mass activity tenfold higher than that of the commercial Pt/C catalyst. This method is simple, fast and potentially versatile, and scalable for the mass production of SACs.

High-yield solid-phase synthesis of humanin, an Alzheimer’s disease associated 24-mer peptide, and humanin analogues

2005 ◽  
Author(s):  
Christos Zikos ◽  
Alexandra Evangelou ◽  
Dimitra Benaki ◽  
Emmanuel Mikros ◽  
Maria Pelecanou ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Solid Phase Synthesis ◽  
Solid Phase ◽  
High Yield ◽  
Phase Synthesis

scholarly journals First Total Synthesis of Hoshinoamides A  

2021 ◽  
Author(s):  
Haipin Zhou ◽  
Zihan Rui ◽  
Yiming Yang ◽  
Shengtao Xu ◽  
Yutian Shao ◽  
...  
Keyword(s):  
Amino Acids ◽  
Plasmodium Falciparum ◽  
Total Synthesis ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis ◽  
High Yield ◽  
Marine Cyanobacterium ◽  
Phase Synthesis ◽  
Synthetic Strategy ◽  
Coupling Yield

Hoshinoamides A, B and C, linear lipopeptides, were isolated from the marine cyanobacterium Caldora penicillata, with potent antiplasmodial activity against chloroquine-sensitive Plasmodium falciparum. Herein, we describe the first total synthesis of Hoshinoamides A. Our synthetic strategy uses the combined methods of solution and solid phase peptide synthesis. Liquid phase synthesis is to improve the coupling yield of L-Val3 and N-Me-D-Phe2. Connecting other amino acids efficiency and convergence by solid state synthesis. This synthetic strategy has good purity and high yield.

scholarly journals First total synthesis of hoshinoamide A

2021 ◽  
Vol 17 ◽  
pp. 2924-2931
Author(s):  
Haipin Zhou ◽  
Zihan Rui ◽  
Yiming Yang ◽  
Shengtao Xu ◽  
Yutian Shao ◽  
...  
Keyword(s):  
Amino Acids ◽  
Liquid Phase ◽  
Total Synthesis ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis ◽  
High Yield ◽  
Marine Cyanobacterium ◽  
Phase Synthesis ◽  
Synthetic Strategy ◽  
Coupling Yield

Hoshinoamides A, B and C, linear lipopeptides, were isolated from the marine cyanobacterium Caldora penicillata, with potent antiplasmodial activity against chloroquine-sensitive Plasmodium falciparum. Herein, we describe the first total synthesis of hoshinoamide A by the combination of liquid-phase and solid-phase peptide synthesis. Liquid-phase synthesis is to improve the coupling yield of ʟ-Val3 and N-Me-ᴅ-Phe2. Connecting other amino acids efficiency and convergence is achieved by solid-state synthesis. Our synthetic strategy could synthesize the target peptide in high yield with good purity

scholarly journals Enzymatic Beacons for Specific Sensing of Dilute Nucleic Acid and Potential Utility for SARS-CoV-2 Detection

2021 ◽  
Author(s):  
Xiaoyu Zhang ◽  
Venubabu Kotikam ◽  
Eriks Rozners ◽  
Brian P. Callahan
Keyword(s):  
Nucleic Acid ◽  
Solid Phase ◽  
Detection Sensitivity ◽  
High Yield ◽  
Nucleic Acid Detection ◽  
Phase Synthesis ◽  
Dna Oligonucleotides ◽  
C Terminus ◽  
Potential Use ◽  
Better Than

Enzymatic beacons, or E-beacons, are 1:1 bioconjugates of the nanoluciferase enzyme linked covalently at its C-terminus to hairpin forming DNA oligonucleotides equipped with a dark quencher. We prepared E-beacons biocatalytically using the promiscuous hedgehog protein-cholesterol ligase, HhC. Instead of cholesterol, HhC attached nanoluciferase site-specifically to mono-sterylated hairpin DNA, prepared in high yield by solid phase synthesis. We tested three potential E-beacon dark quenchers: Iowa Black, Onyx-A, and dabcyl. Prototype E-beacon carrying each of those quenchers provided sequence-specific nucleic acid sensing through turn-on bioluminescence. For practical application, we prepared dabcyl-quenched E-beacons for potential use in detecting the COVID-19 virus, SARS-CoV-2. Targeting the E484 codon of the SARS-CoV-2 Spike protein, E-beacons (80 x 10-12 M) reported wild-type SARS-CoV-2 nucleic acid at greater than or equal to 1 x 10-9 M with increased bioluminescence of 8-fold. E-beacon prepared for the E484K variant of SARS-CoV-2 functioned with similar sensitivity. These E-beacons could discriminate their complementary target from nucleic acid encoding the E484Q mutation of the SARS-CoV-2 Kappa variant. Along with specificity, detection sensitivity with E-beacons is two to three orders of magnitude better than synthetic molecular beacons, rivaling the most sensitive nucleic acid detection agents re-ported to date.

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