Revalorization of adsorbed residual oil in spent bleaching clay as a sole carbon source for polyhydroxyalkanoate (PHA) accumulation in Cupriavidus necator Re2058/pCB113

2020 ◽  
Vol 53 (1) ◽  
pp. 169-178 ◽  
Author(s):  
Nabila Husna Bt Mohamad Hairudin ◽  
Shangeetha Ganesan ◽  
Kumar Sudesh
2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Kenta Yonezuka ◽  
Jun Shimodaira ◽  
Michiro Tabata ◽  
Shun Nagase ◽  
Daisuke Kasai ◽  
...  

Cupriavidus necatorstrain PHE3-6 grows on phenol as a sole carbon source and cometabolizescis-andtrans-dichloroethenes and trichloroethene. Here, we report the draft genome sequence of PHE3-6, which provides insights into the degradation system of phenol and chlorinated ethenes.


2020 ◽  
Vol 69 (12) ◽  
pp. 1619-1626
Author(s):  
Tarique Panhwar ◽  
Sarfaraz Ahmed Mahesar ◽  
Syed Tufail Hussain Sherazi ◽  
Aijaz Ali Laghari ◽  
Abdulaziz E. Atabani

Author(s):  
Mustafa Kamal Abdul Aziz ◽  
Noor Azian Morad ◽  
Noor Baini Nabila Muhamad ◽  
Takayuki Okayama ◽  
Ryota Kose

2021 ◽  
Vol 8 (11) ◽  
pp. 179
Author(s):  
Kenji Tanaka ◽  
Kazumasa Yoshida ◽  
Izumi Orita ◽  
Toshiaki Fukui

The copolyester of 3-hydroxybutyrate (3HB) and 3-hydoxyhexanoate (3HHx), PHBHHx, is one of the most practical kind of bacterial polyhydroxyalkanoates due to its high flexibility and marine biodegradability. PHBHHx is usually produced from vegetable oils or fatty acids through b-oxidation, whereas biosynthesis from sugars has been achieved by recombinant strains of hydrogen-oxidizing bacterium Cupriavidus necator. This study investigated the biosynthesis of PHBHHx from CO2 as the sole carbon source by engineered C. necator strains. The recombinant strains capable of synthesizing PHBHHx from fructose were cultivated in a flask using complete mineral medium and a substrate gas mixture (H2/O2/CO2 = 8:1:1). The results of GC and 1H NMR analyses indicated that the recombinants of C. necator synthesized PHBHHx from CO2 with high cellular content. When 1.0 g/L (NH4)2SO4 was used as a nitrogen source, the 3HHx composition of PHBHHx in the strain MF01∆B1/pBBP-ccrMeJ4a-emd was 47.7 ± 6.2 mol%. Further investigation demonstrated that the PHA composition can be regulated by using (R)-enoyl-CoA hydratase (PhaJ) with different substrate specificity. The composition of 3HHx in PHBHHx was controlled to about 11 mol%, suitable for practical applications, and high cellular content was kept in the strains transformed with pBPP-ccrMeJAc-emd harboring short-chain-length-specific PhaJ.


Author(s):  
Vivek Kumar Ranjan ◽  
Shriparna Mukherjee ◽  
Subarna Thakur ◽  
Krutika Gupta ◽  
Ranadhir Chakraborty

2008 ◽  
Vol 5 (4) ◽  
pp. 367-374 ◽  
Author(s):  
Jing Wang ◽  
Guiwen Yan ◽  
Mingquan An ◽  
Jieli Liu ◽  
Houming Zhang ◽  
...  

1975 ◽  
Vol 25 (2) ◽  
pp. 119-135 ◽  
Author(s):  
Meryl Polkinghorne ◽  
M. J. Hynes

SUMMARYWild-type strains ofAspergillus nidulansgrow poorly onL-histidine as a sole nitrogen source. The synthesis of the enzyme histidase (EC. 4.3.1.3) appears to be a limiting factor in the growth of the wild type, as strains carrying the mutantareA102 allele have elevated histidase levels and grow strongly on histidine as a sole nitrogen source.L-Histidine is an extremely weak sole carbon source for all strains.Ammonium repression has an important role in the regulation of histidase synthesis and the relief of ammonium repression is dependent on the availability of a good carbon source. The level of histidase synthesis does not respond to the addition of exogenous substrate.Mutants carrying lesions in thesarA orsarB loci (suppressor ofareA102) have been isolated. The growth properties of these mutants on histidine as a sole nitrogen source correlate with the levels of histidase synthesized. Mutation at thesarA andsarB loci also reduces the utilization of a number of other nitrogen sources. The data suggest that these two genes may code for regulatory products involved in nitrogen catabolism. No histidase structural gene mutants were identified and possible explanations of this are discussed.


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