scholarly journals Paper microfluidic implementation of loop mediated isothermal amplification for early diagnosis of hepatitis C virus

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Weronika Witkowska McConnell ◽  
Chris Davis ◽  
Suleman R. Sabir ◽  
Alice Garrett ◽  
Amanda Bradley-Stewart ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Early Diagnosis ◽  
Point Of Care ◽  
Disease Diagnosis ◽  
Isothermal Amplification ◽  
World Health ◽  
Nucleic Acid Detection ◽  
Loop Mediated Isothermal Amplification ◽  
User Friendly

AbstractThe early diagnosis of active hepatitis C virus (HCV) infection remains a significant barrier to the treatment of the disease and to preventing the associated significant morbidity and mortality seen, worldwide. Current testing is delayed due to the high cost, long turnaround times and high expertise needed in centralised diagnostic laboratories. Here we demonstrate a user-friendly, low-cost pan-genotypic assay, based upon reverse transcriptase loop mediated isothermal amplification (RT-LAMP). We developed a prototype device for point-of-care use, comprising a LAMP amplification chamber and lateral flow nucleic acid detection strips, giving a visually-read, user-friendly result in <40 min. The developed assay fulfils the current guidelines recommended by World Health Organisation and is manufactured at minimal cost using simple, portable equipment. Further development of the diagnostic test will facilitate linkage between disease diagnosis and treatment, greatly improving patient care pathways and reducing loss to follow-up, so assisting in the global elimination strategy.

scholarly journals Loop-Mediated Isothermal Amplification as Point-of-Care Diagnosis for Neglected Parasitic Infections

2020 ◽  
Vol 21 (21) ◽  
pp. 7981
Author(s):  
Catalina Avendaño ◽  
Manuel Alfonso Patarroyo
Keyword(s):  
Neglected Tropical Diseases ◽  
Point Of Care ◽  
Low Cost ◽  
High Specificity ◽  
World Health Organisation ◽  
Isothermal Amplification ◽  
World Health ◽  
Parasitic Infections ◽  
River Blindness ◽  
Loop Mediated Isothermal Amplification

The World Health Organisation (WHO) has placed twenty diseases into a group known as neglected tropical diseases (NTDs), twelve of them being parasitic diseases: Chagas’ disease, cysticercosis/taeniasis, echinococcosis, food-borne trematodiasis, human African trypanosomiasis (sleeping sickness), leishmaniasis, lymphatic filariasis, onchocerciasis (river blindness), schistosomiasis, soil-transmitted helminthiasis (ascariasis, hookworm, trichuriasis), guinea-worm and scabies. Such diseases affect millions of people in developing countries where one of the main problems concerning the control of these diseases is diagnosis-based due to the most affected areas usually being far from laboratories having suitable infrastructure and/or being equipped with sophisticated equipment. Advances have been made during the last two decades regarding standardising and introducing techniques enabling diagnoses to be made in remote places, i.e., the loop-mediated isothermal amplification (LAMP) technique. This technique’s advantages include being able to perform it using simple equipment, diagnosis made directly in the field, low cost of each test and the technique’s high specificity. Using this technique could thus contribute toward neglected parasite infection (NPI) control and eradication programmes. This review describes the advances made to date regarding LAMP tests, as it has been found that even though several studies have been conducted concerning most NPI, information is scarce for others.

scholarly journals Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay

2011 ◽  
Vol 156 (8) ◽  
pp. 1387-1396 ◽  
Author(s):  
Jin Yang ◽  
Mei-xin Fang ◽  
Jie Li ◽  
Guo-qiang Lou ◽  
Hang-jun Lu ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Assay

A Novel Close-tube Loop Mediated Isothermal Amplification (LAMP) Assay for Rapid Detection of Brucella

2021 ◽  
Author(s):  
Adarsh Mishra ◽  
Prasad Thomas ◽  
Lalit Mohan Jeena ◽  
Soni Doimari ◽  
S. Rajagunalan ◽  
...  
Keyword(s):  
Present Report ◽  
Lamp Assay ◽  
Relative Sensitivity ◽  
Disease Diagnosis ◽  
Isothermal Amplification ◽  
Nucleic Acid Detection ◽  
Brucella Species ◽  
Loop Mediated Isothermal Amplification ◽  
Major Bottleneck ◽  
Therapeutic Measures

Background: Brucellosis is one of the important diseases affecting both human as well as livestock. Rapid diagnosis of the pathogen is highly essential for undertaking effective therapeutic measures. Loop mediated isothermal amplification (LAMP) assay, one of the robust nucleic acid detection platforms, is used especially for rapid disease diagnosis. However, persistent contamination has been the major bottleneck in LAMP which can be eliminated with adoption of appropriate closed-tube formats.Methods: In the present report, two sets of LAMP primers targeting omp2b gene of Brucella were designed and standardized for detection of all the major Brucella spp. Result: There was absence of amplification in any of the non-Brucella species in contrast to detection of white precipitation in unaided eye in daylight as well as greenish fluorescence under ultraviolet light in all of the Brucella species. It was found to be more sensitive than conventional PCR as relative sensitivity was found to be 0.34pg for first set of primers and 34fg for second set of primers, as compared to 3.4pg through bcsp31 PCR. 

scholarly journals Assessing Molecular Point-of-Care Testing and Dried Blood Spot for Hepatitis C Virus Screening in People Who Inject Drugs

2020 ◽  
Vol 7 (6) ◽  
Author(s):  
Stéphane Chevaliez ◽  
Mélanie Wlassow ◽  
Johann Volant ◽  
Françoise Roudot-Thoraval ◽  
Antoine Bachelard ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Whole Blood ◽  
People Who Inject Drugs ◽  
Point Of Care ◽  
Dried Blood Spot ◽  
World Health ◽  
Hcv Rna ◽  
Rna Detection ◽  
Blood Spot

Abstract Background Injecting drug use is a major driver of hepatitis C virus (HCV) spread worldwide, and the World Health Organization (WHO) has identified people who inject drugs (PWID) as a key population to target for HCV screening and care. Point-of-care (POC) hepatitis C tests and dried blood spot (DBS) sampling offer benefits for the management of patients with HCV infection by increasing HCV testing and linkage to care in different nonclinical settings. The aims of this prospective study were to evaluate the feasibility and the acceptability of use HCV ribonucleic acid (RNA) POC and fingerstick DBS testing in social-medical risk-reduction centers and to describe the cascade of care among PWID in France. Methods Between June 2018 and February 2019, 89 consecutive HCV-seropositive PWID attending 2 drug treatment services and 1 supervised consumption room in inner Paris were invited to participate in further evaluation, undergoing a clinical review with a liver assessment and blood tests including fingerstick capillary whole blood POC HCV RNA testing and fingerstick DBS sampling. Results Of the 89 participants enrolled, HCV RNA was detected in 34 (38.6%) participants. Fingerstick whole blood POC RNA testing and HCV RNA detection from DBS sample were feasible and acceptable among PWID with no major difference in terms of HCV RNA detection rate. Overall, 16 participants received pan-genotypic antiviral treatment. The proportion of PWID with sustained virologic response at 12 weeks was 81.2%, with data for 3 patients still pending. Conclusions One-step screening strategy based on the detection of HCV RNA would engage people in care for treatment scale-up and HCV elimination.

scholarly journals Rapid visual detection of hepatitis C virus using a reverse transcription loop-mediated isothermal amplification assay

2021 ◽  
Vol 102 ◽  
pp. 440-445
Author(s):  
Sayamon Hongjaisee ◽  
Natteewan Doungjinda ◽  
Woottichai Khamduang ◽  
Tanawan Samleerat Carraway ◽  
Jiraprapa Wipasa ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Reverse Transcription ◽  
Visual Detection ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Assay

Hepatitis C Virus Infection: A Brief Review

JMS SKIMS ◽  
2020 ◽  
Vol 23 (1) ◽  
pp. 3-15
Author(s):  
Saleem Kamili ◽  
Hisham Qadri
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Basic Research ◽  
World Health ◽  
Hepatitis C Virus Infection ◽  
Poor Countries ◽  
Diagnostic Assays ◽  
Antiviral Therapies ◽  
Highly Sensitive ◽  
Resource Poor

Hepatitis C, caused by hepatitis C virus (HCV) was originally described as parenterally transmitted non-A non-B hepatitis. Since its discovery in 1989, the field of HCV research has become a shining example of successful translation of basic research wherein in a short of span of just 30 years the virus was discovered, highly sensitive and specific diagnostic assays were developed, epidemiology and clinical characteristics of the disease were well defined and now with the availability of highly efficacious antiviral therapies many countries are already on their way to achieving World Health Organization’s (WHO) elimination targets of hepatitis C by 2030.  However, much work needs to be done to eliminate hepatitis C especially in resource poor countries. Most recent data show an estimated 71 million people are currently infected with HCV worldwide and approximately 400,000 people die each year from causes related to HCV. Of these estimates, more than 13 million HCV infected persons are in India and Pakistan (Figure 1). Despite the availability of a cure for hepatitis C, only 20% of those infected patients have been diagnosed (1). In order to achieve the WHO targets of hepatitis C elimination, concerted efforts will have to made to make affordable and reliable diagnostics available worldwide.

Sign in / Sign up

Export Citation Format

Share Document