Proteome-wide analysis of protein lipidation using chemical probes: in-gel fluorescence visualization, identification and quantification of N-myristoylation, N- and S-acylation, O-cholesterylation, S-farnesylation and S-geranylgeranylation

2021 ◽  
Author(s):  
Wouter W. Kallemeijn ◽  
Thomas Lanyon-Hogg ◽  
Nattawadee Panyain ◽  
Andrea Goya Grocin ◽  
Paulina Ciepla ◽  
...  
Keyword(s):  
Chemical Probes ◽  
Protein Lipidation ◽  
Identification And Quantification

scholarly journals Chemical proteomics: a powerful tool for exploring protein lipidation

2013 ◽  
Vol 41 (1) ◽  
pp. 56-61 ◽  
Author(s):  
Elisabeth M. Storck ◽  
Remigiusz A. Serwa ◽  
Edward W. Tate
Keyword(s):  
Present Review ◽  
Chemical Proteomics ◽  
Post Translational Modifications ◽  
Health And Disease ◽  
Protein Lipidation ◽  
Protein Acylation ◽  
Identification And Quantification

The study of post-translational modifications such as protein lipidation is a non-trivial challenge of the post-genomic era. In recent years the field of chemical proteomics has greatly advanced our ability to identify and quantify protein lipidation. In the present review, we give a brief overview of the tools available to study protein acylation, prenylation and cholesterylation, and their application in the identification and quantification of protein lipidation in health and disease.

Development of a Rapid Survey Method of Sampling and Analysis for Asbestos in Ambient Air

1972 ◽  
Vol 30 ◽  
pp. 630-631 ◽  
Author(s):  
R. E. Heffelfinger ◽  
C. W. Melton ◽  
D. L. Kiefer ◽  
W. M. Henry ◽  
R. J. Thompson
Keyword(s):  
Neutron Activation ◽  
Ambient Air ◽  
Survey Method ◽  
Potential Hazard ◽  
Transmission Microscopy ◽  
Comparative Analyses ◽  
Asbestos Fibers ◽  
Controlled Conditions ◽  
Unequivocal Identification ◽  
Identification And Quantification

A methodology has been developed and demonstrated which is capable of determining total amounts of asbestos fibers and fibrils in air ranging from as low as fractional nanograms per cubic meter (ng/m3) of air to several micrograms/m3. The method involves the collection of samples on an absolute filter and provides an unequivocal identification and quantification of the total asbestos contents including fibrils in the collected samples.The developed method depends on the trituration under controlled conditions to reduce the fibers to fibrils, separation of the asbestos fibrils from other collected air particulates (beneficiation), and the use of transmission microscopy for identification and quantification. Its validity has been tested by comparative analyses by neutron activation techniques. It can supply the data needed to set emissions criteria and to serve as a basis for assessing the potential hazard for asbestos pollution to the populace.

HPLC-DAD/ESI-MS identification and quantification of polar constituents in six Stachys taxa from Balkan peninsula

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
A Karioti ◽  
J Kukic Markovic ◽  
S Petrovic ◽  
M Niketic ◽  
A Bilia
Keyword(s):  
Balkan Peninsula ◽  
Esi Ms ◽  
Identification And Quantification
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