Enhancement of immunomodulative effect of lactic acid bacteria on plasmacytoid dendritic cells with sucrose palmitate

AbstractLactococcus lactis strain Plasma (LC-Plasma) possesses strong activity of stimulating plasmacytoid dendritic cells (pDCs) via the TLR9-Myd88 pathway. To reveal the effective genome structure for pDCs stimulatory activity, we performed an in vitro screening, using randomly selected DNA fragments from the LC-Plasma genome. The results showed that CpG motifs are necessary factor for active DNA fragment, but the copy number of CpG motifs did not show strong correlation to the pDCs stimulatory activity of DNA fragment. We also found that the G+C contents of DNA fragments have significant negative effects on pDCs stimulatory activity. We also performed bioinformatics analysis of genome of lactic acid bacteria (LAB) and investigated the relation between CpG copy number in the genome and pDCs stimulatory activity. We found that strains of lactic acid bacteria (LAB) with high copy number of CpG motifs in the low-G+C region of the genome had higher probability of having high pDCs stimulatory activity. Three species, L.lactis subsp. lactis, Leuconostoc mesenteroides, and Pediococcus pentosaceus were the typical examples of high pDCs stimulatory activity LAB.ImportanceThis study provides a new perspective on the structure of DNA fragments that are able to activate pDCs via the TLR9-Myd88 pathway. The information from this study should be useful for designing new DNA fragments, including phosphodiesterbond-DNA oligomers containing CpG motifs and DNA-containing vaccines. This work also presented an in silico screening method for identifying bacterial species that are able to activate pDCs. Therefore, this study should be useful for providing data for the development of vaccine adjuvants and therapeutics for infectious and allergic diseases.

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Impact of Lactic Acid Bacteria on Dendritic Cells from Allergic Patients in an Experimental Model of Intestinal Epithelium

Journal of Biomedicine and Biotechnology ◽

10.1155/2007/71921 ◽

2007 ◽

Vol 2007 ◽

pp. 1-9 ◽

Cited By ~ 10

Author(s):

Céline Ratajczak ◽

Catherine Duez ◽

Corinne Grangette ◽

Pierre Pochard ◽

André-Bernard Tonnel ◽

...

Keyword(s):

Dendritic Cells ◽

Lactic Acid ◽

Lactic Acid Bacteria ◽

Experimental Model ◽

Intestinal Epithelium

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Double-Stranded RNA Derived from Lactic Acid Bacteria Augments Th1 Immunity via Interferon-β from Human Dendritic Cells

Frontiers in Immunology ◽

10.3389/fimmu.2018.00027 ◽

2018 ◽

Vol 9 ◽

Cited By ~ 8

Author(s):

Tadaomi Kawashima ◽

Naho Ikari ◽

Yohei Watanabe ◽

Yoshiro Kubota ◽

Sachiyo Yoshio ◽

...

Keyword(s):

Dendritic Cells ◽

Lactic Acid ◽

Lactic Acid Bacteria ◽

Interferon Β ◽

Double Stranded Rna ◽

Human Dendritic Cells

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Targeting mucosal dendritic cells with microbial antigens from probiotic lactic acid bacteria

Expert Review of Vaccines ◽

10.1586/14760584.7.2.163 ◽

2008 ◽

Vol 7 (2) ◽

pp. 163-174 ◽

Cited By ~ 44

Author(s):

Mansour Mohamadzadeh ◽

Tri Duong ◽

Timothy Hoover ◽

Todd R Klaenhammer

Keyword(s):

Dendritic Cells ◽

Lactic Acid ◽

Lactic Acid Bacteria ◽

Probiotic Lactic Acid Bacteria

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Differential effects of lactobacilli on activation and maturation of mouse dendritic cells

Beneficial Microbes ◽

10.3920/bm2013.0066 ◽

2014 ◽

Vol 5 (3) ◽

pp. 323-334 ◽

Cited By ~ 10

Author(s):

I. Elawadli ◽

J.T. Brisbin ◽

B.A. Mallard ◽

M.W. Griffiths ◽

M. Corredig ◽

...

Keyword(s):

Dendritic Cells ◽

Lactic Acid ◽

Lactic Acid Bacteria ◽

Immune Responses ◽

Inflammatory Cytokine ◽

Cytokine Production ◽

Lactobacillus Helveticus ◽

Inflammatory Cytokine Production

Lactic acid bacteria (LAB) are of interest because of their potential to modulate immune responses. The effects of LAB range from regulation to stimulation of the immune system. A series of studies were performed in vitro to study the effects of six lactic acid bacteria (LAB), Lactobacillus helveticus LH-2, Lactobacillus acidophilus La-5, La-115, La-116 and La-14, and Lactobacillus salivarius, on maturation and activation of mouse dendritic cells. Production of tumour necrosis factor (TNF)-?, interleukin (IL)-6 and IL-10 by dendritic cells (DCs) was determined after treating cells with live LAB. The expression of DC maturation markers, CD80 and CD40, was also measured using flow cytometry after stimulation with LAB. In addition, the expression of Toll-like receptors (TLRs) 2, 4 and 9 by DCs stimulated with LAB was measured. Our results revealed that LAB act differentially on pro-inflammatory and anti-inflammatory cytokine production and induction of co-stimulatory molecules by DCs. Specifically, L. salivarius was found to be the most effective LAB to induce pro-inflammatory cytokine production and expression of co-stimulatory molecules. Moreover, La-14, La-116 and La-5 induced moderate maturation and activation of DCs. On the other hand, LH-2 and La-115 were the least effective lactobacilli to induce DC responses. The present study also revealed that L. salivarius was able to induce the expression of TLR2, 4 and 9 by DCs. In conclusion, various strains and species of LAB can differentially regulate DC activation and maturation, providing further evidence that these bacteria may have the ability to influence and steer immune responses in vivo.

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Effects of oral intake of plasmacytoid dendritic cells-stimulative lactic acid bacterial strain on pathogenesis of influenza-like illness and immunological response to influenza virus

British Journal Of Nutrition ◽

10.1017/s0007114515002408 ◽

2015 ◽

Vol 114 (5) ◽

pp. 727-733 ◽

Cited By ~ 20

Author(s):

Tetsu Sugimura ◽

Hitoshi Takahashi ◽

Kenta Jounai ◽

Konomi Ohshio ◽

Masaya Kanayama ◽

...

Keyword(s):

Dendritic Cells ◽

Influenza Virus ◽

Lactic Acid ◽

Placebo Group ◽

Mononuclear Cells ◽

Winter Season ◽

Plasmacytoid Dendritic Cells ◽

Double Blind ◽

Peripheral Blood Mononuclear ◽

Influenza Like Illness

AbstractLactococcus lactis ssp. lactis JCM5805 has been shown to be a rare lactic acid bacterium that can activate plasmacytoid dendritic cells in both murine and human species. In this study, we carried out a randomised placebo-controlled double-blind experiment to evaluate its effect on the pathogenesis of influenza-like illness during the winter season. A total of 213 volunteers were divided into two groups, which received either yogurt made with L. lactis JCM5805 or a placebo beverage daily for 10 weeks. In the JCM5805 group, the cumulative incidence days of ‘cough’ and ‘feverishness’, which are defined as major symptoms of an influenza-like illness, were significantly decreased compared with the placebo group. In addition, peripheral blood mononuclear cells prepared from volunteers were cultured in the presence of inactivated human influenza virus A/H1N1 (A/PR/8/34). IFN-α elicited by A/H1N1 tended to be higher in the JCM5805 group compared with the placebo group, and an IFN-α-inducible antiviral factor, interferon-stimulated gene 15 (ISG15), elicited by A/H1N1 was significantly higher in the JCM5805 group compared with the placebo group after the intake period. These results suggest that intake of JCM5805 is able to prevent the pathogenesis of an influenza-like illness via enhancement of an IFN-α-mediated response to the influenza virus.

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