AI-powered transmitted light microscopy for functional analysis of live cells

AbstractTransmitted light microscopy can readily visualize the morphology of living cells. Here, we introduce artificial-intelligence-powered transmitted light microscopy (AIM) for subcellular structure identification and labeling-free functional analysis of live cells. AIM provides accurate images of subcellular organelles; allows identification of cellular and functional characteristics (cell type, viability, and maturation stage); and facilitates live cell tracking and multimodality analysis of immune cells in their native form without labeling.

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Quantification of Bacterial Twitching Motility in Dense Colonies Using Transmitted Light Microscopy and Computational Image Analysis

BIO-PROTOCOL ◽

10.21769/bioprotoc.2804 ◽

2018 ◽

Vol 8 (8) ◽

Author(s):

Benjamin Smith ◽

Jianfang Li ◽

Matteo Metruccio ◽

Stephanie Wan ◽

David Evans ◽

...

Keyword(s):

Image Analysis ◽

Light Microscopy ◽

Transmitted Light ◽

Twitching Motility ◽

Transmitted Light Microscopy ◽

Computational Image Analysis

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Thin-Section Preparation and Transmitted-Light Microscopy

10.31399/asm.tb.omfrc.t53030115 ◽

2010 ◽

pp. 115-135

Keyword(s):

Light Microscopy ◽

Polymeric Composites ◽

Glass Slide ◽

Transmitted Light ◽

Fiber Reinforced ◽

Transmitted Light Microscopy ◽

Ultrathin Sections ◽

Microscopy Techniques ◽

Surface Mounting ◽

Selection Of

Abstract Transmitted-light methods reveal more details of the morphology of fiber-reinforced polymeric composites than are observable using any other available microscopy techniques. This chapter describes the various aspects relating to the selection and preparation of ultrathin-section specimens of fiber-reinforced polymeric composites for examination by transmitted-light microscopy techniques. The preparation steps covered are a selection of the rough section, preparation of the rough section for preliminary mounting, grinding and polishing the primary-mount first surface, mounting the first surface on a glass slide, and preparing the second surface (top surface). The optimization of microscope conditions and analysis of specimens by microscopy techniques are also covered. In addition, examples of composite ultrathin sections that are analyzed using transmitted-light microscopy contrast methods are shown throughout.

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Transmitted light microscopy of a fibre reinforced metal

Journal of Microscopy ◽

10.1046/j.1365-2818.2003.01097.x ◽

2003 ◽

Vol 209 (1) ◽

pp. 8-12 ◽

Cited By ~ 6

Author(s):

B. Moser ◽

A. Rossoll ◽

L. Weber ◽

O. Beffort ◽

A. Mortensen

Keyword(s):

Light Microscopy ◽

Transmitted Light ◽

Transmitted Light Microscopy

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Stereoscopic Photography in Transmitted Light Microscopy

Microscopy Today ◽

10.1017/s1551929517000621 ◽

2017 ◽

Vol 25 (4) ◽

pp. 46-49 ◽

Cited By ~ 1

Author(s):

Robert Sturm

Keyword(s):

Light Microscopy ◽

Transmitted Light ◽

Transmitted Light Microscopy

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Jurassic palynostratigraphy of Bornholm, Baltic Sea, Denmark

Danmarks Geologiske Undersøgelse Serie C ◽

10.34194/seriec.v12.7115 ◽

1995 ◽

Vol 12 ◽

pp. 137-144

Author(s):

Eva B Koppelhus

Keyword(s):

Light Microscopy ◽

Baltic Sea ◽

Transmitted Light ◽

Geological Survey ◽

Type Section ◽

Transmitted Light Microscopy ◽

Standard Techniques

Samples of the Rønne Formation have been collected from exposures at Munkerup, Sose Bugt and Galgeløkke and n.vo boreholes, Galgeløkke-1 and -2, at Galgeløkke south of Rønne (Fig. 1). The Hasle Formation was sampled at the type section south of Hasle harbour, at Korsodde and from the Levka-1 and Hasle-1 boreholes. The Baga Formation, that consists of the previously defined Levka, Sorthat and Baga beds (Gry, 1969; Gravesen et al. 1982), was sampled from Mo sections within the Hasle Klinkerfabrik clay pit, from the coastal section at Korsodde, and from four cored boreholes the Levka-1, 106, 107 and 109 at the Hasle Klinkerfabrik clay pit (Nielsen & Koppelhus 1989, 1991; Koppelhus & Nielsen 1994). The samples were processed for their palynological content using the standard techniques developed at the Geological Survey of Denmark (Poulsen et al, 1990). They were examined by transmitted light microscopy. All of the slides are stored in the collections of the Department of Stratigraphy. at the Geological Survey of Denmark.

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Confocal study of microaggregates in 2.5 billion year-old sediments

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100146680 ◽

1993 ◽

Vol 51 ◽

pp. 168-169

Author(s):

G.Y. Fan ◽

S. Mojzsist ◽

G. Arrheniust

Keyword(s):

Confocal Microscopy ◽

Light Microscopy ◽

Western Australia ◽

Transmitted Light ◽

Iron Formation ◽

Drill Core ◽

Thin Sections ◽

Transmitted Light Microscopy ◽

Rock Texture

Light microscopy conducted on samples of Archean sediments reveals microaggregates (Fig. 1) which are suggestive of a biotic origin. These aggregates, typically 15 μm wide and 50 μm long, are thought to be the mineral remains of colonies of microorganisms that lived 2.5 billion years ago. Confocal microscopy is used to study the structures of these microaggregates in 3-D.Samples under study are from the lowest section drill core taken from the Dales Gorge Member of the Brockman Iron-Formation (Hamersley Basin) in Western Australia. These sediments are well preserved and escaped metamorphic conditions typically experienced by older rocks of this type. Two types of samples were prepared for study under the microscope: thin sections (~40 μm) for transmitted light microscopy to study the general rock texture and to locate the features of interest, and thick sections (3 mm) for confocal microscopy to determine the 3-D structure of the aggregates in their undisturbed state.

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