scholarly journals PAK4 methylation by the methyltransferase SETD6 attenuates cell adhesion

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Zlata Vershinin ◽  
Michal Feldman ◽  
Dan Levy
Keyword(s):  
Cell Adhesion ◽  
Mammalian Cells ◽  
Focal Adhesions ◽  
Vital Role ◽  
Migration And Invasion ◽  
Wild Type ◽  
Set Domain ◽  
Cell Adhesion And Migration ◽  
P21 Activated Kinase ◽  
And Migration

Abstract P21-activated kinase 4 (PAK4), a member of serine/threonine kinases family is over-expressed in numerous cancer tumors and is associated with oncogenic cell proliferation, migration and invasion. Our recent work demonstrated that the SET-domain containing protein 6 (SETD6) interacts with and methylates PAK4 at chromatin in mammalian cells, leading to activation of the Wnt/β-catenin signaling pathway. In our current work, we identified lysine 473 (K473) on PAK4 as the primary methylation site by SETD6. Methylation of PAK4 at K473 activates β-catenin transcriptional activity and inhibits cell adhesion. Specific methylation of PAK4 at K473 also attenuates paxillin localization to focal adhesions leading to overall reduction in adhesion-related features, such as filopodia and actin structures. The altered adhesion of the PAK4 wild-type cells is accompanied with a decrease in the migrative and invasive characteristics of the cells. Taken together, our results suggest that methylation of PAK4 at K473 plays a vital role in the regulation of cell adhesion and migration.

scholarly journals The Role of Lipid Rafts in Cancer Cell Adhesion and Migration

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Toshiyuki Murai
Keyword(s):  
Cell Adhesion ◽  
Lipid Rafts ◽  
Cancer Cell ◽  
Cancer Progression ◽  
Migration And Invasion ◽  
Dynamic Features ◽  
Cellular Functions ◽  
Cancer Cell Adhesion ◽  
Cell Adhesion And Migration ◽  
And Migration

Lipid rafts are cholesterol-enriched microdomains of the cell membrane and possess a highly dynamic nature. They have been involved in various cellular functions including the regulation of cell adhesion and membrane signaling through proteins within lipid rafts. The dynamic features of the cancer cell surface may modulate the malignant phenotype of cancer, including adhesion disorders and aggressive phenotypes of migration and invasion. Recently, it was demonstrated that lipid rafts play critical roles in cancer cell adhesion and migration. This article summarizes the important roles of lipid rafts in cancer cell adhesion and migration, with a focus on the current state of knowledge. This article will improve the understanding of cancer progression and lead to the development of novel targets for cancer therapy.

scholarly journals ANGPTL4 overexpression inhibits tumor cell adhesion and migration and predicts favorable prognosis of triple-negative breast cancer

2020 ◽  
Author(s):  
Yu-Chen Cai ◽  
Hang Yang ◽  
Ke-Feng Wang ◽  
Tan-Huan Chen ◽  
Wen-Qi Jiang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Adhesion ◽  
Triple Negative Breast Cancer ◽  
Triple Negative ◽  
Migration And Invasion ◽  
Tumor Cell Adhesion ◽  
Tumor Tissues ◽  
Cell Adhesion And Migration ◽  
And Migration

Abstract Background: Triple-negative breast cancer (TNBC) patients have relatively poor clinical outcomes. A marker predicting the prognosis of patients with TNBC could help guide treatment. Extensive evidence demonstrates that angiopoietin-like 4 (ANGPTL4) is involved in the regulation of cancer growth, metastasis and angiogenesis. Therefore, its role in TNBC is of interest.Methods: We tested the ANGPTL4 expression level in tumor tissues by immunohistochemistry (IHC) and detected its association with the clinical features of TNBC patients. Next, the effects and mechanisms of ANGPTL4 on TNBC cell migration and adhesion were investigated.Results: We found that ANGPTL4 overexpression was associated with favorable outcomes in TNBC patients. ANGPTL4 upregulation inhibited cell adhesion, migration and invasion in vitro. Further analyses demonstrated that the possible mechanism might involve suppression of TNBC progression by interacting with extracellular matrix-related genes.Conclusions: The present findings demonstrated that enhancement of ANGPTL4 expression might inversely correlate with TNBC progression. ANGPTL4 is a promising marker of TNBC and should be evaluated in further studies. Trial registration: Retrospectively registered.

scholarly journals ANGPTL4 overexpression inhibits tumor cell adhesion and migration and predicts favorable prognosis of triple-negative breast cancer

2020 ◽  
Author(s):  
Yu-Chen Cai ◽  
Hang Yang ◽  
Ke-Feng Wang ◽  
Tan-Huan Chen ◽  
Wen-Qi Jiang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Adhesion ◽  
Triple Negative Breast Cancer ◽  
Triple Negative ◽  
Migration And Invasion ◽  
Tumor Cell Adhesion ◽  
Tumor Tissues ◽  
Cell Adhesion And Migration ◽  
And Migration

Abstract Background: Triple-negative breast cancer (TNBC) patients have relatively poor clinical outcomes. A marker predicting the prognosis of patients with TNBC could help guide treatment. Extensive evidence demonstrates that angiopoietin-like 4 (ANGPTL4) is involved in the regulation of cancer growth, metastasis and angiogenesis. Therefore, its role in TNBC is of interest.Methods: We tested the ANGPTL4 expression level in tumor tissues by immunohistochemistry (IHC) and detected its association with the clinical features of TNBC patients. Next, the effects and mechanisms of ANGPTL4 on TNBC cell migration and adhesion were investigated.Results: We found that ANGPTL4 overexpression was associated with favorable outcomes in TNBC patients. ANGPTL4 upregulation inhibited cell adhesion, migration and invasion in vitro. Further analyses demonstrated that the possible mechanism might involve suppression of TNBC progression by interacting with extracellular matrix-related genes.Conclusions: The present findings demonstrated that enhancement of ANGPTL4 expression might inversely correlate with TNBC progression. ANGPTL4 is a promising marker of TNBC and should be evaluated in further studies. Trial registration: Retrospectively registered.

scholarly journals ANGPTL4 overexpression inhibits tumor cell adhesion and migration and predicts favorable prognosis of triple-negative breast cancer

BMC Cancer ◽  
2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Yu-Chen Cai ◽  
Hang Yang ◽  
Ke-Feng Wang ◽  
Tan-Huan Chen ◽  
Wen-Qi Jiang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Adhesion ◽  
Triple Negative Breast Cancer ◽  
Triple Negative ◽  
Migration And Invasion ◽  
Tumor Cell Adhesion ◽  
Tumor Tissues ◽  
Cell Adhesion And Migration ◽  
And Migration

Abstract Background Triple-negative breast cancer (TNBC) patients have relatively poor clinical outcomes. A marker predicting the prognosis of patients with TNBC could help guide treatment. Extensive evidence demonstrates that angiopoietin-like 4 (ANGPTL4) is involved in the regulation of cancer growth, metastasis and angiogenesis. Therefore, its role in TNBC is of interest. Methods: We tested the ANGPTL4 expression level in tumor tissues by immunohistochemistry (IHC) and detected its association with the clinical features of TNBC patients. Next, the effects and mechanisms of ANGPTL4 on TNBC cell migration and adhesion were investigated. Results We found that ANGPTL4 overexpression was associated with favorable outcomes in TNBC patients. ANGPTL4 upregulation inhibited cell adhesion, migration and invasion in vitro. Further analyses demonstrated that the possible mechanism might involve suppression of TNBC progression by interacting with extracellular matrix-related genes. Conclusions The present findings demonstrated that enhancement of ANGPTL4 expression might inversely correlate with TNBC progression. ANGPTL4 is a promising marker of TNBC and should be evaluated in further studies. Trial registration Retrospectively registered.

scholarly journals GAR22β regulates cell migration, sperm motility, and axoneme structure

2016 ◽  
Vol 27 (2) ◽  
pp. 277-294 ◽  
Author(s):  
Ivonne Gamper ◽  
David Fleck ◽  
Meltem Barlin ◽  
Marc Spehr ◽  
Sara El Sayad ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Cell Motility ◽  
Focal Adhesion ◽  
Wild Type ◽  
Cell Adhesion And Migration ◽  
Cytoskeleton Remodeling ◽  
Focal Adhesion Turnover ◽  
And Migration ◽  
Noncanonical Amino Acid ◽  
Dependent Processes

Spatiotemporal cytoskeleton remodeling is pivotal for cell adhesion and migration. Here we investigated the function of Gas2-related protein on chromosome 22 (GAR22β), a poorly characterized protein that interacts with actin and microtubules. Primary and immortalized GAR22β−/− Sertoli cells moved faster than wild-type cells. In addition, GAR22β−/− cells showed a more prominent focal adhesion turnover. GAR22β overexpression or its reexpression in GAR22β−/− cells reduced cell motility and focal adhesion turnover. GAR22β–actin interaction was stronger than GAR22β–microtubule interaction, resulting in GAR22β localization and dynamics that mirrored those of the actin cytoskeleton. Mechanistically, GAR22β interacted with the regulator of microtubule dynamics end-binding protein 1 (EB1) via a novel noncanonical amino acid sequence, and this GAR22β–EB1 interaction was required for the ability of GAR22β to modulate cell motility. We found that GAR22β is highly expressed in mouse testes, and its absence resulted in reduced spermatozoa generation, lower actin levels in testes, and impaired motility and ultrastructural disorganization of spermatozoa. Collectively our findings identify GAR22β as a novel regulator of cell adhesion and migration and provide a foundation for understanding the molecular basis of diverse cytoskeleton-dependent processes.

scholarly journals ANGPTL4 overexpression inhibits tumor cell adhesion and migration and predicts favorable prognosis of triple-negative breast cancer

2020 ◽  
Author(s):  
Yu-Chen Cai ◽  
Hang Yang ◽  
Ke-Feng Wang ◽  
Tan-Huan Chen ◽  
Wen-Qi Jiang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Adhesion ◽  
Triple Negative Breast Cancer ◽  
Triple Negative ◽  
Migration And Invasion ◽  
Tumor Cell Adhesion ◽  
Tumor Tissues ◽  
Cell Adhesion And Migration ◽  
And Migration

Abstract Background: Triple-negative breast cancer (TNBC) patients have relatively poor clinical outcomes. A marker predicting the prognosis of patients with TNBC could help guide treatment. Extensive evidence demonstrates that angiopoietin-like 4 (ANGPTL4) is involved in the regulation of cancer growth, metastasis and angiogenesis. Therefore, its role in TNBC is of interest. Methods: We tested the ANGPTL4 expression level in tumor tissues by immunohistochemistry (IHC) and detected its association with the clinical features of TNBC patients. Next, the effects and mechanisms of ANGPTL4 on TNBC cell migration and adhesion were investigated. Results: We found that ANGPTL4 overexpression was associated with favorable outcomes in TNBC patients. ANGPTL4 upregulation inhibited cell adhesion, migration and invasion in vitro. Further analyses demonstrated that the possible mechanism might involve suppression of TNBC progression by interacting with extracellular matrix-related genes. Conclusions: The present findings demonstrated that enhancement of ANGPTL4 expression might inversely correlate with TNBC progression. ANGPTL4 is a promising marker of TNBC and should be evaluated in further studies. Trial registration: Retrospectively registered.

scholarly journals Regulation of the microtubular cytoskeleton by Polycystin-1 favors focal adhesions turnover to modulate cell adhesion and migration

2015 ◽  
Vol 16 (1) ◽  
Author(s):  
Maddalena Castelli ◽  
Chiara De Pascalis ◽  
Gianfranco Distefano ◽  
Nadia Ducano ◽  
Amanda Oldani ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Focal Adhesions ◽  
Microtubular Cytoskeleton ◽  
Cell Adhesion And Migration ◽  
And Migration

scholarly journals ANGPTL4 Overexpression Inhibits Tumor Cell Adhesion and Migration and Predicts Favorable Prognosis of Triple-Negative Breast Cancer

2020 ◽  
Author(s):  
Yu-Chen Cai ◽  
Hang Yang ◽  
Ke-Feng Wang ◽  
Tan-Huan Chen ◽  
Wen-Qi Jiang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Adhesion ◽  
Triple Negative Breast Cancer ◽  
Triple Negative ◽  
Migration And Invasion ◽  
Tumor Cell Adhesion ◽  
Tumor Tissues ◽  
Cell Adhesion And Migration ◽  
And Migration

Abstract Background: Triple-negative breast cancer (TNBC) patients have relatively poor clinical outcomes. A marker predicting the prognosis of patients with TNBC could help guide treatment. Extensive evidence demonstrates that angiopoietin-like 4 (ANGPTL4) is involved in the regulation of cancer growth, metastasis and angiogenesis. Therefore, its role in TNBC is of interest.Methods: We tested the ANGPTL4 expression level in tumor tissues by immunohistochemistry (IHC) and detected its association with the clinical features of TNBC patients. Next, the effects and mechanisms of ANGPTL4 on TNBC cell migration and adhesion were investigated.Results: We found that ANGPTL4 overexpression was associated with favorable outcomes in TNBC patients. ANGPTL4 upregulation inhibited cell adhesion, migration and invasion in vitro. Further analyses demonstrated that the possible mechanism might involve suppression of TNBC progression by interacting with extracellular matrix-related genes.Conclusions: The present findings demonstrated that enhancement of ANGPTL4 expression might inversely correlate with TNBC progression. ANGPTL4 is a promising marker of TNBC and should be evaluated in further studies. Trial registration: Retrospectively registered.

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