scholarly journals Octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Saskia Seiser ◽  
Lukas Janker ◽  
Nina Zila ◽  
Michael Mildner ◽  
Ana Rakita ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Skin ◽  
Ex Vivo ◽  
Skin Wound ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tape Stripping ◽  
Skin Wound Healing ◽  
Wound Model ◽  
Octenidine Dihydrochloride ◽  
Anti Inflammatory

AbstractOctenidine dihydrochloride (OCT) is a widely used antiseptic molecule, promoting skin wound healing accompanied with improved scar quality after surgical procedures. However, the mechanisms by which OCT is contributing to tissue regeneration are not yet completely clear. In this study, we have used a superficial wound model by tape stripping of ex vivo human skin. Protein profiles of wounded skin biopsies treated with OCT-containing hydrogel and the released secretome were analyzed using liquid chromatography-mass spectrometry (LC–MS) and enzyme-linked immunosorbent assay (ELISA), respectively. Proteomics analysis of OCT-treated skin wounds revealed significant lower levels of key players in tissue remodeling as well as reepithelization after wounding such as pro-inflammatory cytokines (IL-8, IL-6) and matrix-metalloproteinases (MMP1, MMP2, MMP3, MMP9) when compared to controls. In addition, enzymatic activity of several released MMPs into culture supernatants was significantly lower in OCT-treated samples. Our data give insights on the mode of action based on which OCT positively influences wound healing and identified anti-inflammatory and protease-inhibitory activities of OCT.

scholarly journals Parakeratotic Corneocytes Play a Unique Role in Human Skin Wound Healing

2013 ◽  
Vol 133 (3) ◽  
pp. 856-858 ◽  
Author(s):  
Marcia L. Usui ◽  
Robert A. Underwood ◽  
Philip. Fleckman ◽  
John E. Olerud
Keyword(s):  
Wound Healing ◽  
Human Skin ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Unique Role

scholarly journals Human skin long noncoding RNA WAKMAR1 regulates wound healing by enhancing keratinocyte migration

2019 ◽  
Vol 116 (19) ◽  
pp. 9443-9452 ◽  
Author(s):  
Dongqing Li ◽  
Lara Kular ◽  
Manika Vij ◽  
Eva K. Herter ◽  
Xi Li ◽  
...  
Keyword(s):  
Wound Healing ◽  
Noncoding Rna ◽  
Chronic Wounds ◽  
Ex Vivo ◽  
Dna Methyltransferases ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Protein Coding ◽  
Keratinocyte Migration ◽  
Gene Expression Control

An increasing number of studies reveal the importance of long noncoding RNAs (lncRNAs) in gene expression control underlying many physiological and pathological processes. However, their role in skin wound healing remains poorly understood. Our study focused on a skin-specific lncRNA, LOC105372576, whose expression was increased during physiological wound healing. In human nonhealing wounds, however, its level was significantly lower compared with normal wounds under reepithelialization. We characterized LOC105372576 as a nuclear-localized, RNAPII-transcribed, and polyadenylated lncRNA. In keratinocytes, its expression was induced by TGF-β signaling. Knockdown of LOC105372576 and activation of its endogenous transcription, respectively, reduced and increased the motility of keratinocytes and reepithelialization of human ex vivo skin wounds. Therefore, LOC105372576 was termed “wound and keratinocyte migration-associated lncRNA 1” (WAKMAR1). Further study revealed that WAKMAR1 regulated a network of protein-coding genes important for cell migration, most of which were under the control of transcription factor E2F1. Mechanistically, WAKMAR1 enhanced E2F1 expression by interfering with E2F1 promoter methylation through the sequestration of DNA methyltransferases. Collectively, we have identified a lncRNA important for keratinocyte migration, whose deficiency may be involved in the pathogenesis of chronic wounds.

scholarly journals Nigella sativa and Its Active Compound, Thymoquinone, Accelerate Wound Healing in an In Vivo Animal Model: A Comprehensive Review

2020 ◽  
Vol 17 (11) ◽  
pp. 4160 ◽  
Author(s):  
Nusaibah Sallehuddin ◽  
Abid Nordin ◽  
Ruszymah Bt Hj Idrus ◽  
Mh Busra Fauzi
Keyword(s):  
Wound Healing ◽  
Nigella Sativa ◽  
Healing Process ◽  
Skin Wound ◽  
Tissue Growth ◽  
Inclusion Criteria ◽  
Skin Wound Healing ◽  
In Vivo Animal Model ◽  
Anti Inflammatory

Nigella sativa (NS) has been reported to have a therapeutic effect towards skin wound healing via its anti-inflammatory, tissue growth stimulation, and antioxidative properties. This review examines all the available studies on the association of Nigella sativa (NS) and skin wound healing. The search was performed in Medline via EBSCOhost and Scopus databases to retrieve the related papers released between 1970 and March 2020. The principal inclusion criteria were original article issued in English that stated wound healing criteria of in vivo skin model with topically applied NS. The search discovered 10 related articles that fulfilled the required inclusion criteria. Studies included comprise different types of wounds, namely excisional, burn, and diabetic wounds. Seven studies unravelled positive results associated with NS on skin wound healing. Thymoquinone has anti-inflammatory, antioxidant, and antibacterial properties, which mainly contributed to wound healing process.

scholarly journals 350 The landscape of microRNA-mediated gene regulation in human skin wound healing and venous ulcers

2021 ◽  
Vol 141 (10) ◽  
pp. S210
Author(s):  
Z. Liu ◽  
L. Zhang ◽  
MA. Toma ◽  
P. Sommar ◽  
N.X. Landén
Keyword(s):  
Wound Healing ◽  
Gene Regulation ◽  
Human Skin ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Venous Ulcers

scholarly journals 1′‐Acetoxychavicol acetate accelerates human skin wound healing

2015 ◽  
Vol 29 (S1) ◽  
Author(s):  
Atsuki Mori ◽  
Koji Sugawara ◽  
Daisuke Tsuruta ◽  
Isao Matsui‐Yuasa ◽  
Akiko Kojima‐Yuasa
Keyword(s):  
Wound Healing ◽  
Human Skin ◽  
Skin Wound ◽  
Skin Wound Healing
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