scholarly journals Overwintering honeybees maintained dynamic and stable intestinal bacteria

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Peng Liu ◽  
Yujie Zhu ◽  
Liang Ye ◽  
Tengfei Shi ◽  
Lai Li ◽  
...  
Keyword(s):  
16S Rdna ◽  
High Throughput ◽  
Beta Diversity ◽  
High Throughput Sequencing ◽  
Intestinal Bacteria ◽  
High Quality ◽  
Dynamic Changes ◽  
Ecological Balance ◽  
Significant Difference

AbstractHoneybee is an important pollinator for maintaining ecological balance. However, scientist found the bizarre mass death of bees in winter. Meanwhile, some reported that the differences composed of intestinal bacteria between healthy honeybees and CCD honeybees. It is essential that explored dynamic changes to the intestinal bacteria in overwintering honeybees. We collected bee samples before overwintering, during prophase of overwintering, metaphase of overwintering, anaphase of overwintering, telophase of overwintering, and after overwintering. By using high-throughput sequencing targeting the V3−V4 regions of the 16S rDNA, the abundance of the intestinal bacteria were analyzed in overwintering honeybees. A total of 1,373,886 high-quality sequences were acquired and Proteobacteria (85.69%), Firmicutes (10.40%), Actinobacteria (3.66%), and Cyanobacteria (1.87%) were identified as major components of the intestinal bacteria. All core honeybee intestinal bacteria genera, such as Gilliamella, Bartonella, Snodgrassella, Lactobacillus, Frischella, Commensalibacter, and Bifidobacterium were detected. The abundance of Actinobacteria, Bartonella, and Bifidobacterium increased initially and then decreased in winter honeybees. There were no significant differences in the richness and evenness of the microbiota in overwintering honeybees; however, there was a statistically significant difference in the beta diversity of the intestinal bacteria after overwintering compared with that in other groups. Our results suggested that honeybees maintained their intestinal ecosystem balance, and increased the abundance of gut probiotics in response to environmental and nutrition pressures in winter.

scholarly journals Overwintering honeybees maintained dynamic and stable intestinal bacteria

2021 ◽  
Author(s):  
Peng Liu ◽  
Yujie Zhu ◽  
Liang Ye ◽  
Tengfei Shi ◽  
Lai Li ◽  
...  
Keyword(s):  
16S Rdna ◽  
High Throughput ◽  
Beta Diversity ◽  
High Throughput Sequencing ◽  
Intestinal Bacteria ◽  
High Quality ◽  
Dynamic Changes ◽  
Ecological Balance ◽  
Significant Difference

Abstract Honeybee is an important pollinator for maintaining ecological balance. However, scientist found the bizarre mass death of bees in winter. Meanwhile, some reported that the differences composed of intestinal bacteria between healthy honeybees and CCD honeybees. It is essential that explored dynamic changes to the intestinal bacteria in overwintering honeybees. We collected bee samples before overwintering, during prophase of overwintering, metaphase of overwintering, anaphase of overwintering, telophase of overwintering, and after overwintering. By using high-throughput sequencing targeting the V3 − V4 regions of the 16S rDNA, the abundance of the intestinal bacteria were analyzed in overwintering honeybees. A total of 1,373,886 high-quality sequences were acquired and Proteobacteria (85.69%), Firmicutes (10.40%), Actinobacteria (3.66%), and Cyanobacteria (1.87%) were identified as major components of the intestinal bacteria. All core honeybee intestinal bacteria genera, such as Gilliamella, Bartonella, Snodgrassella, Lactobacillus, Frischella, Commensalibacter, and Bifidobacterium were detected. The abundance of Actinobacteria, Bartonella, and Bifidobacterium increased initially and then decreased in winter honeybees. There were no significant differences in the richness and evenness of the microbiota in overwintering honeybees; however, there was a statistically significant difference in the beta diversity of the intestinal bacteria after overwintering compared with that in other groups. Our results suggested that honeybees maintained their intestinal ecosystem balance, and increased the abundance of gut probiotics in response to environmental and nutrition pressures in winter.

scholarly journals Overwintering Honeybees Maintained Dynamic and Stable Intestinal Bacteria

2021 ◽  
Author(s):  
Peng Liu ◽  
Yujie Zhu ◽  
Liang Ye ◽  
Tengfei Shi ◽  
Lai Li ◽  
...  
Keyword(s):  
16S Rdna ◽  
High Throughput ◽  
Beta Diversity ◽  
High Throughput Sequencing ◽  
Intestinal Bacteria ◽  
High Quality ◽  
Dynamic Changes ◽  
Ecological Balance ◽  
Significant Difference

Abstract Honeybee is an important pollinator for maintaining ecological balance. However, scientist found the bizarre mass death of bees in winter. Meanwhile, some reported that the differences composed of intestinal bacteria between healthy honeybees and CCD honeybees. It is essential that explored dynamic changes to the intestinal bacteria in overwintering honeybees. We collected bee samples before overwintering, during prophase of overwintering, metaphase of overwintering, anaphase of overwintering, telophase of overwintering, and after overwintering. By using high-throughput sequencing targeting the V3 − V4 regions of the 16S rDNA, the abundance of the intestinal bacteria were analyzed in overwintering honeybees. A total of 1,373,886 high-quality sequences were acquired and Proteobacteria (85.69%), Firmicutes (10.40%), Actinobacteria (3.66%), and Cyanobacteria (1.87%) were identified as major components of the intestinal bacteria. All core honeybee intestinal bacteria genera, such as Gilliamella, Bartonella, Snodgrassella, Lactobacillus, Frischella, Commensalibacter, and Bifidobacterium were detected. The abundance of Actinobacteria, Bartonella, and Bifidobacterium increased initially and then decreased in winter honeybees. There were no significant differences in the richness and evenness of the microbiota in overwintering honeybees; however, there was a statistically significant difference in the beta diversity of the intestinal bacteria after overwintering compared with that in other groups. Our results suggested that honeybees maintained their intestinal ecosystem balance, and increased the abundance of gut probiotics in response to environmental and nutrition pressures in winter.

Effects of perioperative managements on ocular surface microbiota in intravitreal injection patients

2021 ◽  
Author(s):  
Yaguang Hu ◽  
Qiong Wu ◽  
Fang Sui ◽  
Ming Zhang ◽  
Zhen Zhang ◽  
...  
Keyword(s):  
High Throughput ◽  
Ocular Surface ◽  
Beta Diversity ◽  
High Throughput Sequencing ◽  
Intravitreal Injections ◽  
Shaanxi Province ◽  
Eye Drops ◽  
Species Classification ◽  
Sequencing Platform ◽  
Significant Difference

Abstract Background: We investigated the effects of perioperative managements on ocular surface microbiota in patients who received intravitreal injections.Methods: Samples of ocular surface microbiota were obtained from 41 eyes of 41 patients who received intravitreal injections at the First Affiliated Hospital of Xi'an Jiaotong University and People’s Hospital in Shaanxi Province. The microbial 16s rDNA from samples were analyzed using an Illumina MiSeq high-throughput sequencing platform. Operating classification units (OTU) clustering and alpha/beta diversity analysis was performed for species classification.Results: High-throughput sequencing yielded 1697337 sequences and 1396, indicating an equivalent number of ocular surface microbiota. OTU was significantly lower in the eyes that received intravitreal injections, compared with those who did not received injections (P<0.05). The number of OTUs was inversely correlated with the administered of perioperative managements and the times of intravitreal injections. Beta diversity showed a significant difference among each groups (P<0.05). With the increased perioperative managements, the prevalence of gram-positive bacteria (Clostridium) had decreased and the prevalence of gram-negative bacteria (Neisseria) had increased.Conclusions: Microbiota on the ocular surface were significantly different between treated and control eyes. And the composition of microbiota on the ocular surface was dramatically changed over time in eyes that received intravitreal injections. Perioperative managements might disrupt the balance of the ocular surface microbiota, increasing risk of infectious disease. Therefore, perioperative managements including local antibiotic eye drops should be applied cautiously before intraocular surgery.

scholarly journals Assessing diversity of the female urine microbiota by high throughput sequencing of 16S rDNA amplicons

2011 ◽  
Vol 11 (1) ◽  
pp. 244 ◽  
Author(s):  
Huma Siddiqui ◽  
Alexander J Nederbragt ◽  
Karin Lagesen ◽  
Stig L Jeansson ◽  
Kjetill S Jakobsen
Keyword(s):  
16S Rdna ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Female Urine

scholarly journals Comparison of invertebrate diversity in lake waters and their resting eggs in sediments, as revealed by high-throughput sequencing (HTS)

2020 ◽  
pp. 19
Author(s):  
Xiaoyan Wang ◽  
Qing Wang ◽  
Yufeng Yang ◽  
Wenbo Yu
Keyword(s):  
High Throughput ◽  
Water Samples ◽  
High Throughput Sequencing ◽  
Essential Feature ◽  
Resting Eggs ◽  
Freshwater Ecosystems ◽  
Rrna Gene ◽  
Promising Alternative ◽  
Invertebrate Diversity ◽  
Significant Difference

Aquatic invertebrate diversity reflects water quality and the health of aquatic ecosystems and should be monitored as an essential feature of freshwater ecosystems. The resting eggs of aquatic invertebrates in sediments populate the overlying water. The diversity of invertebrates in waters and their resting eggs in sediments in Baiyangdian Lake, Xiongan, North China, were assessed using high-throughput sequencing (HTS) with a pair of 18S rRNA gene adaptor-linked primers. The total of 99 operational taxonomic units (OTUs) derived from 353,755 invertebrate sequences (mostly zooplankton) were revealed by this study. A total of 50 species in the water samples including 20 rotifers, 11 copepods, 1 cladoceran and 18 other species were sorted out. In the sediment 37 species, including 21 rotifers, 3 copepods, 1 cladoceran and 12 other species, were identified. There were 24 species in common between water and corresponding sediments. Invertebrate OTU richness in water samples was higher than that in sediments (p < 0.01), while there was no significant difference in the Shannon-Wiener index. These results suggest that HTS is a promising alternative for efficient biodiversity assessment and monitoring.

Specific microbes of saliva and vaginal fluid of Guangdong Han females based on 16S rDNA high-throughput sequencing

2019 ◽  
Vol 133 (3) ◽  
pp. 699-710 ◽  
Author(s):  
Hongyan Huang ◽  
Ting Yao ◽  
Weibin Wu ◽  
Chuangyan Zhai ◽  
Tianshan Guan ◽  
...  
Keyword(s):  
16S Rdna ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Vaginal Fluid

Analysis of the effects of nanosilver on bacterial community in the intestinal fluid of silkworms using high-throughput sequencing

2019 ◽  
Vol 110 (3) ◽  
pp. 309-320
Author(s):  
Chen Lin ◽  
Zhou Wei ◽  
Zhou Yi ◽  
Tan Tingting ◽  
Du Huamao ◽  
...  
Keyword(s):  
Bacterial Community ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Intestinal Bacteria ◽  
Illumina Miseq ◽  
The Body ◽  
Dependent Manner ◽  
Negative Effects ◽  
Silkworm Larvae ◽  
Intestinal Bacterial Community

AbstractNanosilver is an environment-friendly, harmless alternative of traditional disinfectants which can be potentially applied in the sericulture industry. However, the effects of nanosilver on the intestinal bacterial community of the silkworms (Bombyx mori L.) are unclear. In this study, Illumina MiSeq high-throughput sequencing technology was used to assess the intestinal bacterial community in both male and female silkworms while treated with different concentrations of nanosilver. We found that nanosilver significantly influenced the composition of silkworm intestinal bacterial community on the different taxonomic levels. Most conspicuously, the abundance of Firmicutes was increased by the treatment of 20 mg L−1 nanosilver but decreased by that of 100 mg L−1 nanosilver at the phylum level. The same trend was observed in Bacilli at the class level and in Enterococcus at the genus level. In some extreme cases, application of nanosilver eliminated the bacterium, e.g., Brevibacillus, but increased the population of several other bacteria in the host intestine, such as Blautia, Terrisporobacter, Faecalibacterium, and some bacteria could only be found in nanosilver treatment groups, e.g., Dialister. In addition, although nanosilver generally showed negative effects on the cocooning rate in a dose-dependent manner, we found that 20 mg L−1 nanosilver treatment significantly increased the body weight of silkworms and did not show negative effects on the survival rate. These results indicated that the intestinal bacteria community of silkworm larvae was significantly changed after nanosilver treatment which might consequently influence host growth and development.

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