scholarly journals Proteomic profiling of human amnion for preterm birth biomarker discovery

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Maurizio Bruschi ◽  
Martina Bartolucci ◽  
Andrea Petretto ◽  
Francesca Buffelli ◽  
Xhuliana Kajana ◽  
...  
Keyword(s):  
Preterm Birth ◽  
Inflammatory Response ◽  
Biomarker Discovery ◽  
Predictive Biomarkers ◽  
Maternal And Newborn Health ◽  
Spontaneous Preterm Birth ◽  
Bioinformatics Analyses ◽  
Core Proteins ◽  
Matrix Metallopeptidase ◽  
Matrix Metallopeptidase 9

AbstractSpontaneous preterm birth (PTB) complicates about 12% of pregnancies worldwide, remaining the main cause of neonatal morbidity and mortality. Spontaneous preterm birth PTBs is often caused by microbial-induced preterm labor, mediated by an inflammatory process threatening both maternal and newborn health. In search for novel predictive biomarkers of PTB and preterm prelabor rupture of the membranes (pPROM), and to improve understanding of infection related PTB, we performed an untargeted mass spectrometry discovery study on 51 bioptic mid zone amnion samples from premature babies. A total of 6352 proteins were identified. Bioinformatics analyses revealed a ranked core of 159 proteins maximizing the discrimination between the selected clinical stratification groups allowing to distinguish conditions of absent (FIR 0) from maximal Fetal Inflammatory Response (FIR 3) stratified in function of Maternal Inflammatory Response (MIR) grade. Matrix metallopeptidase-9 (MMP-9) was the top differentially expressed protein. Gene Ontology enrichment analysis of the core proteins showed significant changes in the biological pathways associated to inflammation and regulation of immune and infection response. Data suggest that the conditions determining PTB would be a transversal event, secondary to the maternal inflammatory response causing a breakdown in fetal-maternal tolerance, with fetal inflammation being more severe than maternal one. We also highlight matrix metallopeptidase-9 as a potential predictive biomarker of PTB that can be assayed in the maternal serum, for future investigation.

scholarly journals Spontaneous preterm birth in African Americans is associated with infection and inflammatory response gene variants

2009 ◽  
Vol 200 (2) ◽  
pp. 209.e1-209.e27 ◽  
Author(s):  
Digna R. Velez ◽  
Stephen Fortunato ◽  
Poul Thorsen ◽  
Salvatore J. Lombardi ◽  
Scott M. Williams ◽  
...  
Keyword(s):  
Preterm Birth ◽  
African Americans ◽  
Inflammatory Response ◽  
Gene Variants ◽  
Spontaneous Preterm Birth ◽  
Response Gene

168: Impaired anti-inflammatory response in women with a prior spontaneous preterm birth

2008 ◽  
Vol 199 (6) ◽  
pp. S59
Author(s):  
Luisa Wetta ◽  
Alice Goepfert ◽  
Elena Lobashevskaya ◽  
Suzanne Cliver ◽  
Joseph Biggio ◽  
...  
Keyword(s):  
Preterm Birth ◽  
Inflammatory Response ◽  
Spontaneous Preterm Birth ◽  
Anti Inflammatory

scholarly journals Adiponectin and Omentin Levels as Predictive Biomarkers of Preterm Birth in Patients with Gestational Diabetes Mellitus

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Radzisław Mierzyński ◽  
Dominik Dłuski ◽  
Łukasz Nowakowski ◽  
Elżbieta Poniedziałek-Czajkowska ◽  
Bożena Leszczyńska-Gorzelak
Keyword(s):  
Diabetes Mellitus ◽  
Preterm Birth ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Preterm Labor ◽  
Predictive Biomarkers ◽  
Control Group ◽  
Strong Correlations ◽  
Spontaneous Preterm Birth ◽  
Clinical Biomarker

Objective. The aim of this study was to determine any changes in adiponectin and omentin levels in GDM patients who delivered at term and preterm and to evaluate whether adipokines can be useful as a clinical biomarker to predict subsequent preterm delivery. Patients and Methods. The levels of adiponectin and omentin were measured in four groups: (1) women with GDM who delivered at term (n=63); (2) women with GDM who had the symptoms of threatened preterm labor and delivered at term (n=23); (3) women with GDM and spontaneous preterm birth (before 37 completed weeks of gestation) (n=19); (4) women with physiological pregnancy (n=55). Results. In comparison with control group the median adiponectin concentrations were significantly lower in all GDM groups (10737 versus 8879; 7057; 6253 ng/ml, respectively; p<0.01). The median omentin concentrations were also significantly lower in all GDM groups in comparison with control group (469 versus 432; 357; 308 ng/ml, respectively; p<0.01). No significant differences in adiponectin and omentin levels between the GDM, preterm labor, and preterm birth groups were observed. However, there was a trend towards lower adiponectin and omentin levels in preterm birth group. The strong correlations between adiponectin and omentin levels were observed in all groups (R=0.801, p<0.001; R=0.824, p<0.001; R=0.705, p<0.001; R=0.764, respectively; p<0.001). In the univariable logistic regression model, significant correlation between omentin concentrations and preterm birth occurrence was found. Conclusions. Our findings suggest that omentin-1, rather than adiponectin, could be useful as a predictor of preterm birth in patients with gestational diabetes mellitus.

Transcriptome changes in maternal peripheral blood during term parturition mimic perturbations preceding spontaneous preterm birth

2021 ◽  
Author(s):  
Nardhy Gomez-Lopez ◽  
Roberto Romero ◽  
Jose Galaz ◽  
Gaurav Bhatti ◽  
Bogdan Done ◽  
...  
Keyword(s):  
Preterm Birth ◽  
Peripheral Blood ◽  
Predictive Biomarkers ◽  
Cervical Ripening ◽  
Strongly Correlated ◽  
Uterine Contractility ◽  
Spontaneous Preterm Birth ◽  
Maternal Circulation ◽  
Maternal Peripheral Blood ◽  
Transcriptomic Changes

Abstract The complex physiologic process of parturition includes the onset of labor, which requires the orchestrated stimulation of a common pathway involving uterine contractility, cervical ripening, and chorioamniotic membrane activation. However, the labor-specific processes taking place in these tissues have limited use as predictive biomarkers unless they can be probed in non-invasive samples, such as the peripheral blood. Herein, we utilized a transcriptomic dataset to assess labor-specific changes in the peripheral blood of women who delivered at term. We identified a set of genes that were differentially expressed with labor and enriched for immunological processes, and these gene expression changes were strongly correlated with results from prior studies, providing in silico validation of our findings. We then identified significant correlations between labor-specific transcriptomic changes in the maternal circulation and those detected in the chorioamniotic membranes, myometrium, and cervix of women at term, demonstrating that tissue-specific labor signatures are partly mirrored in the peripheral blood. Finally, we demonstrated a significant overlap between the peripheral blood transcriptomic changes in term parturition and those observed in asymptomatic women, prior to the diagnosis of preterm prelabor rupture of the membranes, who ultimately delivered preterm. Collectively, we provide evidence that the normal process of labor at term is characterized by a unique immunological expression signature, which may serve as a useful tool for assessing labor status and for potentially identifying women at risk for preterm birth.

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