Interactive Effects of Plant Growth-Promoting Rhizobacteria and Phosphates Sources on Growth and Phosphorus Nutrition of Soybean under Moderate Drought

The growth of legume plants is usually improved by the rhizobacteria inoculation under low phosphorus (P) and alleviation of P nutrition plays important role in plant drought stress response. The aim of this study was to assess the comparative efficacy of two plant growth promoting rhizobacteria namely Burkholderia cepacia B36 and Enterobacter radicincitans D5/23T combined with two sources of phosphates in soybean (Glycine max L.) under low water supply. Plants were grown under P soluble versus insoluble P fertilization for comparing the effects of soybean inoculation on growth, uptake and use efficiency of phosphorus under moderate drought stress. At the beginning of flowering, half of plants was subjected to low water supply (35% water holding capacity, WHC) for 12 days while control plants were well watered - 70% WHC. The plants were harvested at the end of drought and physiological traits and P contents were analyzed. The inoculation treatments showed better plant growth and nutrient uptake when compared to uninoculated control. The application of the Burkholderia cepacia was more efficiently in terms plant growth than E. radicincitans especially under insoluble phosphates. Phosphorus concentrations of shoots and roots increased with both bacterial strains. The bacterial inoculation has much better stimulatory effect on nutrient uptake by soybean fertilized with insoluble phosphates. Study findings indicate that the combined application of PGPR (Burkholderia cepacia B36) and P amendments has the potential to improve P nutrition and growth of soybean cultivated on P-deficient soil under well-watered as well as moderate drought condition. ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 3, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

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Enhancement of drought tolerance in diverse Vicia faba cultivars by inoculation with plant growth-promoting rhizobacteria under newly reclaimed soil conditions

Scientific Reports ◽

10.1038/s41598-021-02847-2 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Elsayed Mansour ◽

Hany A. M. Mahgoub ◽

Samir A. Mahgoub ◽

El-Sayed E. A. El-Sobky ◽

Mohamed I. Abdul-Hamid ◽

...

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Plant Growth ◽

Plant Growth Promoting Rhizobacteria ◽

Severe Drought ◽

Drought Conditions ◽

Moderate Drought ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Effects Of Drought

AbstractWater deficit has devastating impacts on legume production, particularly with the current abrupt climate changes in arid environments. The application of plant growth-promoting rhizobacteria (PGPR) is an effective approach for producing natural nitrogen and attenuating the detrimental effects of drought stress. This study investigated the influence of inoculation with the PGPR Rhizobium leguminosarum biovar viciae (USDA 2435) and Pseudomonas putida (RA MTCC5279) solely or in combination on the physio-biochemical and agronomic traits of five diverse Vicia faba cultivars under well-watered (100% crop evapotranspiration [ETc]), moderate drought (75% ETc), and severe drought (50% ETc) conditions in newly reclaimed poor-fertility sandy soil. Drought stress substantially reduced the expression of photosynthetic pigments and water relation parameters. In contrast, antioxidant enzyme activities and osmoprotectants were considerably increased in plants under drought stress compared with those in well-watered plants. These adverse effects of drought stress reduced crop water productivity (CWP) and seed yield‐related traits. However, the application of PGPR, particularly a consortium of both strains, improved these parameters and increased seed yield and CWP. The evaluated cultivars displayed varied tolerance to drought stress: Giza-843 and Giza-716 had the highest tolerance under well-watered and moderate drought conditions, whereas Giza-843 and Sakha-4 were more tolerant under severe drought conditions. Thus, co-inoculation of drought-tolerant cultivars with R. leguminosarum and P. putida enhanced their tolerance and increased their yield and CWP under water-deficit stress conditions. This study showed for the first time that the combined use of R. leguminosarum and P. putida is a promising and ecofriendly strategy for increasing drought tolerance in legume crops.

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Effect of the inoculation of plant growth-promoting rhizobacteria on the photosynthetic characteristics of Sambucus williamsii Hance container seedlings under drought stress

AMB Express ◽

10.1186/s13568-019-0899-x ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 2

Author(s):

Fangchun Liu ◽

Hailin Ma ◽

Lin Peng ◽

Zhenyu Du ◽

Bingyao Ma ◽

...

Keyword(s):

Water Stress ◽

Drought Stress ◽

Plant Growth ◽

Water Supply ◽

Plant Growth Promoting Rhizobacteria ◽

Drought Duration ◽

Chl A ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Photosynthetic Properties

Abstract Plant growth-promoting rhizobacteria (PGPR) are beneficial bacteria that survive within the range of plant rhizosphere and can promote plant growth. The effects of PGPR in promoting plant growth, activating soil nutrients, reducing fertilizer application, and improving the resistance of plant inducible system have been widely investigated. However, few studies have investigated PGPR as elicitors of tolerance to abiotic stresses, especially drought stress. In this study, the effects of Acinetobacter calcoaceticus X128 on the photosynthetic rate (Pn), stomatal conductance (Gs), intracellular CO2 concentration (Ci), and total chlorophyll content [Chl(a+b)] of Sambucus williamsii Hance seedling leaves under moderate drought stress and drought-rewatering conditions were determined. Compared with those of uninoculated seedlings, the average Pn values during the entire drought stress of inoculated seedlings increased by 12.99%. As the drought duration was lengthened, Ci of uninoculated leaves continued to increase after rapidly declining, whereas Gs continuously decreased. Furthermore, their photosynthetic properties were simultaneously restricted by stomatal and non-stomatal factors. After X128 inoculation, Ci and Gs of S. williamsii Hance leaves continued to decrease, and their photosynthetic properties were mainly restricted by stomatal factors. At the end of the drought stress, water stress reduced [Chl(a + b)] of S. williamsii Hance leaves by 13.49%. However, X128 inoculation decreased this deficit to only 7.39%. After water supply was recovered, Pn, Gs, and [Chl(a+b)] in uninoculated leaves were reduced by 14.23%, 12.02%, and 5.86%, respectively, relative to those under well-watered conditions. However, Ci increased by 6.48%. Compared with those of uninoculated seedlings, Pn, Gs, and [Chl(a+b)] in X128-inoculated seedlings were increased by 9.83%, 9.30%, and 6.85%, respectively. Therefore, the inoculation of X128 under arid environments can mitigate the reduction of chlorophyll, delay the restriction caused by non-stomatal factors to Pn in plant leaves under water stress, and can be more conducive to the recovery of photosynthetic functions of leaves after water supply is recovered.

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Pengaruh Aplikasi Plant Growth Promoting Rhizobacteria terhadap Pertumbuhan dan Hasil Tanaman Bawang Merah (Allium cepa L. Aggregatum group)

Vegetalika ◽

10.22146/veg.47812 ◽

2020 ◽

Vol 9 (4) ◽

pp. 512

Author(s):

Nanda Dwi Hafri ◽

Endang Sulistyaningsih ◽

Arif Wibowo

Keyword(s):

Bacillus Subtilis ◽

Plant Growth ◽

Allium Cepa ◽

Burkholderia Cepacia ◽

Plant Growth Promoting Rhizobacteria ◽

Bacillus Methylotrophicus ◽

Plant Growth Promoting ◽

Growth Promoting

Salah satu upaya penanganan penyakit moler bawang merah dilakukan melalui aplikasi Trichoderma. Aplikasi Trichoderma pada bawang merah memiliki beberapa keunggulan, yaitu mampu mensintesis hormon pertumbuhan tanaman. Terdapat jenis mikroba lain yang juga mampu meningkatkan fitohormon pada tanaman, yaitu Plant Growth Promoting Rhizobacteria (PGPR). Tujuan dari penelitian ini adalah untuk mengetahui dan menentukan isolat PGPR yang memiliki pengaruh paling baik terhadap pertumbuhan dan hasil bawang merah varietas Crok Kuning di lahan sawah. Penelitian ini menggunakan Rancangan Acak Kelompok Lengkap (RAKL) faktor tunggal dengan tiga blok sebagai ulangan. Faktor perlakuan yang digunakan adalah lima isolat PGPR, yaitu: Bp.25.7 Bacillus subtilis, BrSG.5 Bacillus amyloliquofaciens, Bp.25.2 Bacillus methylotrophicus, BrsM.4 Burkholderia cepacia, danBp.25.6 Bacillus amyloliquofaciens dengan dua kontrol, yaitu kontrol positif berupa Trichoderma dan kontrol negatif tanpa aplikasi perlakuan. Hasil penelitian menunjukkan bahwa pemberian perlakuan isolat Bp.25.2 Bacillus methylotrophicus pada bawang merah menyebabkan Laju Asimilasi Bersih (LAB) bawang merah lebih tinggi dibandingkan dengan pemberian perlakuan empat isolat PGPR lainnya maupun kontrol, tetapi sama baiknya dengan pemberian perlakuan Trichoderma. LAB yang tinggi menyebabkan Laju Pertumbuhan Tanaman (LPT) bawang merah dengan pemberian perlakuan Bp.25.2 Bacillus methylotrophicus yang lebih tinggi dibandingkan dengan BrSG.5 Bacillus amyloliquofaciens dan Bp.25.6 Bacillus amyloliquofaciens, tetapi sama baiknya dengan pemberian perlakuan dua isolat PGPR lainnya, kontrol, maupun Trichoderma. Pemberian aplikasi lima isolat PGPR sama baiknya dengan aplikasi Trichoderma dalam meningkatkan pertumbuhan tanaman bawang merah pada variabel luas permukaan daun dan bobot kering total dibandingkan kontrol. Peningkatan variabel pertumbuhan ini tidak diikuti oleh peningkatan variabel hasil dan produktivitas bawang merah tidak berbeda nyata antar perlakuan.

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