scholarly journals Sonoporation generates downstream cellular impact after membrane resealing

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xinxing Duan ◽  
Qian Zhou ◽  
Jennifer M. F. Wan ◽  
Alfred C. H. Yu
Keyword(s):  
Exposure Period ◽  
Acoustic Energy ◽  
Hsp 70 ◽  
Pulsed Ultrasound ◽  
Ultrasound Exposure ◽  
Peak Negative Pressure ◽  
Therapeutic Protocols ◽  
Membrane Resealing ◽  
New Evidence ◽  
Acoustic Energy Density

AbstractSonoporation via microbubble-mediated ultrasound exposure has shown potential in drug and gene delivery. However, there is a general lack of mechanistic knowledge on sonoporation-induced cellular impact after membrane resealing, and this issue has made it challenging to apply sonoporation efficiently in practice. Here, we present new evidence on how sonoporation, without endangering immediate cell viability, may disrupt downstream cellular hemostasis in ways that are distinguished from the bioeffects observed in other sonicated and unsonoporated cells. Sonoporation was realized on HL-60 leukemia cells by delivering pulsed ultrasound (1 MHz frequency, 0.50 MPa peak negative pressure; 10% duty cycle; 30 s exposure period; 29.1 J/cm2 acoustic energy density) in the presence of lipid-shelled microbubbles (1:1 cell-to-bubble ratio). Results showed that 54.6% of sonoporated cells, despite remaining initially viable, underwent apoptosis or necrosis at 24 h after sonoporation. Anti-proliferation behavior was also observed in sonoporated cells as their subpopulation size was reduced by 43.8% over 24 h. Preceding these cytotoxic events, the percentages of sonoporated cells in different cell cycle phases were found to be altered by 12 h after exposure. As well, for sonoporated cells, their expressions of cytoprotective genes in the heat shock protein-70 (HSP-70) family were upregulated by at least 4.1 fold at 3 h after exposure. Taken altogether, these findings indicate that sonoporated cells attempted to restore homeostasis after membrane resealing, but many of them ultimately failed to recover. Such mechanistic knowledge should be taken into account to devise more efficient sonoporation-mediated therapeutic protocols.

Structural Source Identification from Acoustic Measurements Using an Energetic Approach

2017 ◽  
Vol 34 (4) ◽  
pp. 431-441 ◽  
Author(s):  
A. Samet ◽  
M. A. Ben Souf ◽  
O. Bareille ◽  
M. N. Ichchou ◽  
T. Fakhfakh ◽  
...  
Keyword(s):  
Energy Method ◽  
Numerical Test ◽  
Acoustic Energy ◽  
Vibration Source ◽  
Radiated Noise ◽  
Acoustic Cavity ◽  
Energetic Approach ◽  
Structural Force ◽  
Noise Measurements ◽  
Acoustic Energy Density

AbstractAn inverse energy method for the identification of the structural force in high frequency ranges from radiated noise measurements is presented in this paper. The radiation of acoustic energy of the structure coupled to an acoustic cavity is treated using an energetic method called the simplified energy method. The main novelty of this paper consists in using the same energy method to solve inverse structural problem. It consists of localization and quantification of the vibration source through the knowledge of acoustic energy density. Numerical test cases with different measurement points are used for validation purpose. The numerical results show that the proposed method has an excellent performance in detecting the structural force with a few acoustical measurements.

scholarly journals Cardiopulmonary function in rats with lung hemorrhage induced by pulsed ultrasound exposure.

2001 ◽  
Vol 20 (11) ◽  
pp. 1197-1206 ◽  
Author(s):  
J M Kramer ◽  
T G Waldrop ◽  
L A Frizzell ◽  
J F Zachary ◽  
W D O'Brien
Keyword(s):  
Pulsed Ultrasound ◽  
Ultrasound Exposure ◽  
Cardiopulmonary Function ◽  
Lung Hemorrhage

An Energy Boundary Element Formulation for Predicting the Acoustic Field Around a Vehicle at High Frequencies Due to Airborne Noise Sources

2003 ◽  
Author(s):  
Aimin Wang ◽  
Nickolas Vlahopoulos ◽  
Jason Zhu ◽  
Mike Qian
Keyword(s):  
Energy Density ◽  
Boundary Element ◽  
Acoustic Field ◽  
Test Data ◽  
Acoustic Energy ◽  
Element Formulation ◽  
Acoustic Intensity ◽  
Numerical Predictions ◽  
Airborne Noise ◽  
Acoustic Energy Density

An Energy Boundary Element Analysis (EBEA) formulation is presented for calculating sound radiation from a source with arbitrary shape at high frequency. The basic integral equation for the EBEA is derived including a half-space boundary condition. The time and frequency averaged acoustic energy density and acoustic intensity constitutes the primary variables of the new formulation, and the corresponding Green’s functions are derived. The governing equations for the EBEA are established and the numerical formulae for the coefficients of the system matrix, the acoustic energy density, and the acoustic intensity are derived using a Gaussian quadrature. The EBEA formulation and the corresponding numerical implementation are validated by comparing EBEA results to test data for the acoustic field around a vehicle that originates from an airborne noise source. Good correlation is demonstrated between numerical predictions and test data.

An adaptive equalization scheme using acoustic energy density

2004 ◽  
Vol 115 (5) ◽  
pp. 2612-2612
Author(s):  
Xi Chen ◽  
Scott D. Sommerfeldt ◽  
Timothy W. Leishman
Keyword(s):  
Energy Density ◽  
Acoustic Energy ◽  
Adaptive Equalization ◽  
Acoustic Energy Density

scholarly journals The effect of acoustic radiation force on osteoblasts in cell/hydrogel constructs for bone repair

2016 ◽  
Vol 241 (10) ◽  
pp. 1149-1156 ◽  
Author(s):  
James Veronick ◽  
Fayekah Assanah ◽  
Lakshmi S Nair ◽  
Varun Vyas ◽  
Bryan Huey ◽  
...  
Keyword(s):  
Bone Repair ◽  
Acoustic Radiation ◽  
Radiation Force ◽  
Fracture Repair ◽  
Acoustic Energy ◽  
Invasive Technique ◽  
Bony Defect ◽  
Pulsed Ultrasound ◽  
Physical Force ◽  
Non Union

Ultrasound, or the application of acoustic energy, is a minimally invasive technique that has been used in diagnostic, surgical, imaging, and therapeutic applications. Low-intensity pulsed ultrasound (LIPUS) has been used to accelerate bone fracture repair and to heal non-union defects. While shown to be effective the precise mechanism behind its utility is still poorly understood. In this study, we considered the possibility that LIPUS may be providing a physical stimulus to cells within bony defects. We have also evaluated ultrasound as a means of producing a transdermal physical force that could stimulate osteoblasts that had been encapsulated within collagen hydrogels and delivered to bony defects. Here we show that ultrasound does indeed produce a measurable physical force and when applied to hydrogels causes their deformation, more so as ultrasound intensity was increased or hydrogel stiffness decreased. MC3T3 mouse osteoblast cells were then encapsulated within hydrogels to measure the response to this force. Statistically significant elevated gene expression for alkaline phosphatase and osteocalcin, both well-established markers of osteoblast differentiation, was noted in encapsulated osteoblasts (p < 0.05), suggesting that the physical force provided by ultrasound may induce bone formation in part through physically stimulating cells. We have also shown that this osteoblastic response is dependent in part on the stiffness of the encapsulating hydrogel, as stiffer hydrogels resulted in reducing or reversing this response. Taken together this approach, encapsulating cells for implantation into a bony defect that can potentially be transdermally loaded using ultrasound presents a novel regenerative engineering approach to enhanced fracture repair.

TREATMENT OF HOOK OF THE HAMATE FRACTURES IN ADULTS USING LOW-INTENSITY PULSED ULTRASOUND

Hand Surgery ◽  
2014 ◽  
Vol 19 (03) ◽  
pp. 433-436 ◽  
Author(s):  
Michiyo Tomaru ◽  
Denju Osada ◽  
Satoshi Fujita ◽  
Kazuya Tamai
Keyword(s):  
Computed Tomography ◽  
Carpal Tunnel ◽  
Daily Activities ◽  
Bony Union ◽  
Pulsed Ultrasound ◽  
Ultrasound Exposure ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Hypothenar Eminence

Two delayed unions and one nonunion of hook of the hamate fractures in adults aged 31-, 40-, and 41-years-old were treated with low-intensity pulsed ultrasound (LIPUS). Ultrasound treatment was started at three, four, and six months after injury and ultrasound exposure at the hook of the hamate in the hypothenar eminence was carried out for four to five months. During the management period, there was no immobilisation with a cast or brace and limited strain with only routine daily activities allowed. In all cases, bony union was confirmed on carpal tunnel radiographs or computed tomography at the final follow-up time of eight and 36 months after injuries.

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