scholarly journals Aging selectively dampens oscillation of lipid abundance in white and brown adipose tissue

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ntsiki M. Held ◽  
M. Renate Buijink ◽  
Hyung L. Elfrink ◽  
Sander Kooijman ◽  
Georges E. Janssens ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Lipid Metabolism ◽  
Acyl Chain ◽  
Lipid Homeostasis ◽  
Cardiometabolic Health ◽  
Acyl Chain Length ◽  
Brown Adipose ◽  
Tissue Lipids ◽  
Specific Regulation ◽  
Accumulation Of Lipids

AbstractLipid metabolism is under the control of the circadian system and circadian dysregulation has been linked to obesity and dyslipidemia. These factors and outcomes have also been associated to, or affected by, the process of aging. Here, we investigated whether murine white (WAT) and brown (BAT) adipose tissue lipids exhibit rhythmicity and if this is affected by aging. To this end, we have measured the 24 h lipid profiles of WAT and BAT using a global lipidomics analysis of > 1100 lipids. We observed rhythmicity in nearly all lipid classes including glycerolipids, glycerophospholipids, sterol lipids and sphingolipids. Overall, ~ 22% of the analyzed lipids were considered rhythmic in WAT and BAT. Despite a general accumulation of lipids upon aging the fraction of oscillating lipids decreased in both tissues to 14% and 18%, respectively. Diurnal profiles of lipids in BAT appeared to depend on the lipid acyl chain length and this specific regulation was lost in aged mice. Our study revealed how aging affects the rhythmicity of lipid metabolism and could contribute to the quest for targets that improve diurnal lipid homeostasis to maintain cardiometabolic health during aging.

scholarly journals Aging selectively dampens oscillation of lipid abundance in white and brown adipose tissue

2020 ◽  
Author(s):  
Ntsiki M. Held ◽  
M. Renate Buijink ◽  
Hyung L. Elfrink ◽  
Sander Kooijman ◽  
Georges E. Janssens ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Lipid Metabolism ◽  
Acyl Chain ◽  
Lipid Homeostasis ◽  
Cardiometabolic Health ◽  
Acyl Chain Length ◽  
Brown Adipose ◽  
Tissue Lipids ◽  
Specific Regulation ◽  
Accumulation Of Lipids

AbstractLipid metabolism is under the control of the circadian system and circadian dysregulation has been linked to obesity and dyslipidemia. These factors and outcomes have also been associated to, or affected by, the process of aging. Here, we investigated whether murine white (WAT) and brown (BAT) adipose tissue lipids exhibit rhythmicity and if this is affected by aging. To this end, we have measured the 24 hour lipid profiles of WAT and BAT using a global lipidomics analysis of >1100 lipids. We observed rhythmicity in nearly all lipid classes including glycerolipids, glycerophospholipids, sterol lipids and sphingolipids. Overall, ~22% of the analyzed lipids were considered rhythmic in WAT and BAT. Despite a general accumulation of lipids upon aging the fraction of oscillating lipids decreased in both tissues to 14% and 18%, respectively. Diurnal profiles of lipids in BAT appeared to depend on the lipid acyl chain length and this specific regulation was lost in aged mice. Our study revealed how aging affects the rhythmicity of lipid metabolism and could contribute to the quest for targets that improve diurnal lipid homeostasis to maintain cardiometabolic health during aging.

P53 regulates the lipid metabolism response to metabolic stress in brown adipose tissue

2020 ◽  
Author(s):  
G Lenihan-Geels ◽  
F Garcia-Carrizo ◽  
C Li ◽  
M Oster ◽  
A Prokesch ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Lipid Metabolism ◽  
Brown Adipose Tissue ◽  
Metabolic Stress ◽  
Brown Adipose

scholarly journals Apolipoprotein A-IV Enhances Fatty Acid Uptake by Adipose Tissues of Male Mice via Sympathetic Activation

Endocrinology ◽  
2020 ◽  
Vol 161 (4) ◽  
Author(s):  
Qi Zhu ◽  
Jonathan Weng ◽  
Minqian Shen ◽  
Jace Fish ◽  
Zhujun Shen ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Fatty Acid ◽  
Lipid Metabolism ◽  
Sympathetic Activity ◽  
Sympathetic Innervation ◽  
Acid Uptake ◽  
Adipose Tissues ◽  
Lipid Mixture ◽  
Apolipoprotein A ◽  
Brown Adipose

Abstract Apolipoprotein A-IV (ApoA-IV) synthesized by the gut regulates lipid metabolism. Sympathetic innervation of adipose tissues also controls lipid metabolism. We hypothesized that ApoA-IV required sympathetic innervation to increase fatty acid (FA) uptake by adipose tissues and brown adipose tissue (BAT) thermogenesis. After 3 weeks feeding of either a standard chow diet or a high-fat diet (HFD), mice with unilateral denervation of adipose tissues received intraperitoneal administration of recombinant ApoA-IV protein and intravenous infusion of lipid mixture with radioactive triolein. In chow-fed mice, ApoA-IV administration increased FA uptake by intact BAT but not the contralateral denervated BAT or intact white adipose tissue (WAT). Immunoblots showed that, in chow-fed mice, ApoA-IV increased expression of lipoprotein lipase and tyrosine hydroxylase in both intact BAT and inguinal WAT (IWAT), while ApoA-IV enhanced protein levels of β3 adrenergic receptor, adipose triglyceride lipase, and uncoupling protein 1 in the intact BAT only. In HFD-fed mice, ApoA-IV elevated FA uptake by intact epididymal WAT (EWAT) but not intact BAT or IWAT. ApoA-IV increased sympathetic activity assessed by norepinephrine turnover (NETO) rate in BAT and EWAT of chow-fed mice, whereas it elevated NETO only in EWAT of HFD-fed mice. These observations suggest that, in chow-fed mice, ApoA-IV activates sympathetic activity of BAT and increases FA uptake by BAT via innervation, while in HFD-fed mice, ApoA-IV stimulates sympathetic activity of EWAT to shunt FAs into the EWAT.

scholarly journals Identification of functional lipid metabolism biomarkers of brown adipose tissue aging

2019 ◽  
Vol 24 ◽  
pp. 1-17 ◽  
Author(s):  
Sabrina Gohlke ◽  
Vyacheslav Zagoriy ◽  
Alvaro Cuadros Inostroza ◽  
Michaël Méret ◽  
Carola Mancini ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Lipid Metabolism ◽  
Brown Adipose Tissue ◽  
Brown Adipose ◽  
Tissue Aging

scholarly journals Brown adipose tissue and lipid metabolism imaging

Methods ◽  
2017 ◽  
Vol 130 ◽  
pp. 105-113 ◽  
Author(s):  
Andreas Paulus ◽  
Wouter van Marken Lichtenbelt ◽  
Felix M. Mottaghy ◽  
Matthias Bauwens
Keyword(s):  
Adipose Tissue ◽  
Lipid Metabolism ◽  
Brown Adipose Tissue ◽  
Brown Adipose

scholarly journals The role of active brown adipose tissue (aBAT) in lipid metabolism in healthy Chinese adults

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
Xiaoliang Shao ◽  
Wei Yang ◽  
Xiaonan Shao ◽  
Chun Qiu ◽  
Xiaosong Wang ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Lipid Metabolism ◽  
Brown Adipose Tissue ◽  
Chinese Adults ◽  
Brown Adipose ◽  
Healthy Chinese

scholarly journals PPARγ regulates adipose triglyceride lipase in adipocytes in vitro and in vivo

2007 ◽  
Vol 293 (6) ◽  
pp. E1736-E1745 ◽  
Author(s):  
Erin E. Kershaw ◽  
Michael Schupp ◽  
Hong-Ping Guan ◽  
Noah P. Gardner ◽  
Mitchell A. Lazar ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Adipose Tissue ◽  
Lipid Metabolism ◽  
Protein Synthesis Inhibitor ◽  
Adipose Triglyceride Lipase ◽  
Dependent Manner ◽  
Triglyceride Lipase ◽  
Brown Adipose

Peroxisome proliferator-activated receptor-γ (PPARγ) regulates adipocyte genes involved in adipogenesis and lipid metabolism and is the molecular target for thiazolidinedione (TZD) antidiabetic agents. Adipose triglyceride lipase (ATGL) is a recently described triglyceride-specific lipase that is induced during adipogenesis and remains highly expressed in mature adipocytes. This study evaluates the ability of PPARγ to directly regulate ATGL expression in adipocytes in vitro and in vivo. In fully differentiated 3T3-L1 adipocytes, ATGL mRNA and protein are increased by TZD and non-TZD PPARγ agonists in a dose- and time-dependent manner. Rosiglitazone-mediated induction of ATGL mRNA is rapid and is not inhibited by the protein synthesis inhibitor cycloheximide, indicating that intervening protein synthesis is not required for this effect. Rosiglitazone-mediated induction of ATGL mRNA and protein is inhibited by the PPARγ-specific antagonist GW-9662 and is also significantly reduced following siRNA-mediated knockdown of PPARγ, supporting the direct transcriptional regulation of ATGL by PPARγ. In vivo, ATGL mRNA and protein are increased by rosiglitazone treatment in white and brown adipose tissue of mice with and without obesity due to high-fat diet or leptin deficiency. Thus, PPARγ positively regulates ATGL mRNA and protein expression in mature adipocytes in vitro and in adipose tissue in vivo, suggesting a role for ATGL in mediating PPARγ's effects on lipid metabolism.

USF1 deficiency activates brown adipose tissue and improves cardiometabolic health

2016 ◽  
Vol 8 (323) ◽  
pp. 323ra13-323ra13 ◽  
Author(s):  
Pirkka-Pekka Laurila ◽  
Jarkko Soronen ◽  
Sander Kooijman ◽  
Saara Forsström ◽  
Mariëtte R. Boon ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Cardiometabolic Health ◽  
Brown Adipose

scholarly journals Multiple effects of cold exposure on livers of male mice

2018 ◽  
Vol 238 (2) ◽  
pp. 91-106 ◽  
Author(s):  
Aldo Grefhorst ◽  
Johanna C van den Beukel ◽  
Wieneke Dijk ◽  
Jacobie Steenbergen ◽  
Gardi J Voortman ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Lipid Metabolism ◽  
Cold Exposure ◽  
Ldl Receptor ◽  
Whole Body ◽  
Hepatic Lipid Metabolism ◽  
Hepatic Lipid ◽  
Hepatic Expression ◽  
Brown Adipose ◽  
Whole Body Metabolism

Cold exposure of mice is a common method to stimulate brown adipose tissue (BAT) activity and induce browning of white adipose tissue (WAT) that has beneficial effects on whole-body lipid metabolism, including reduced plasma triglyceride (TG) concentrations. The liver is a key regulatory organ in lipid metabolism as it can take up as well as oxidize fatty acids. The liver can also synthesize, store and secrete TGs in VLDL particles. The effects of cold exposure on murine hepatic lipid metabolism have not been addressed. Here, we report the effects of 24-h exposure to 4°C on parameters of hepatic lipid metabolism of male C57BL/6J mice. Cold exposure increased hepatic TG concentrations by 2-fold (P < 0.05) but reduced hepatic lipogenic gene expression. Hepatic expression of genes encoding proteins involved in cholesterol synthesis and uptake such as the LDL receptor (LDLR) was significantly increased upon cold exposure. Hepatic expression of Cyp7a1 encoding the rate-limiting enzyme in the classical bile acid (BA) synthesis pathway was increased by 4.3-fold (P < 0.05). Hepatic BA concentrations and fecal BA excretion were increased by 2.8- and 1.3-fold, respectively (P < 0.05 for both). VLDL-TG secretion was reduced by approximately 50% after 24 h of cold exposure (P < 0.05). In conclusion, cold exposure has various, likely intertwined effects on the liver that should be taken into account when studying the effects of cold exposure on whole-body metabolism.

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