scholarly journals Lipidomic profiling of the developing kernel clarifies the lipid metabolism of Paeonia ostii

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Shui-Yan Yu ◽  
Ying Zhang ◽  
Yu-Ping Lyu ◽  
Zu-Jie Yao ◽  
Yong-Hong Hu
Keyword(s):  
Phosphatidyl Ethanolamine ◽  
Phosphatidyl Inositol ◽  
Phosphatidyl Serine ◽  
Sphingolipid Metabolism ◽  
Phosphatidyl Glycerol ◽  
Alpha Linolenic Acid ◽  
Oil Accumulation ◽  
Oil Crops ◽  
Sphingosine 1 Phosphate ◽  
Fatty Acyls

AbstractLipid components in the developing kernel of Paeonia ostii were determined, and the fatty acid (FA) distributions in triacylglycerol and phospholipids were characterized. The lipids in the kernel were mainly phospholipids (43%), neutral glycerides (24%), fatty acyls (26%), and sphingolipids (4.5%). The dominant neutral glycerides were TAG and diacylglycerol. The PL components included phosphatidic acid, phosphatidyl glycerol, phosphatidyl choline, phosphatidyl serine, phosphatidyl inositol, and phosphatidyl ethanolamine. As the kernel developed, the profiles of the molecular species comprising TAG and PL changed, especially during the earlier phases of oil accumulation. During rapid oil accumulation, the abundances of sphingosine-1-phosphate, pyruvic acid, stearic acid, and alpha-linolenic acid changed significantly; the sphingolipid metabolism and unsaturated FAs biosynthesis pathways were significantly enriched in these differentially abundant metabolites. Our results improve our understanding of lipid accumulation in tree peony seeds, and provide a framework for the analysis of lipid metabolisms in other oil crops.

Effects of cholesterol on growth and lipid composition of Pythium sp. PRL 2142

1972 ◽  
Vol 18 (7) ◽  
pp. 1059-1063 ◽  
Author(s):  
J. A. Brushaber ◽  
J. J. Child ◽  
R. H. Haskins
Keyword(s):  
Growth Rate ◽  
Phosphatidyl Ethanolamine ◽  
Initial Period ◽  
Lipid Synthesis ◽  
Phosphatidyl Inositol ◽  
Dry Weight ◽  
Phosphatidyl Serine ◽  
Phosphatidyl Glycerol ◽  
Ethanolamine Phosphatidyl ◽  
Weight Yield

Addition of exogenous cholesterol to Pythium initially increases the growth rate, but the final dry weight yield is reduced. Cholesterol induces an overall increase in lipid synthesis after the initial period of rapid growth. The lipid content of cholesterol-grown mycelium becomes about double that of mycelium grown without cholesterol. The proportion of phosphatidyl serine relative to other phospholipids is reduced by half in mycelia grown with cholesterol. The major phospholipids are phosphatidyl ethanolamine, phosphatidyl serine, and phosphatidyl choline. Minor phospholipids identified are phosphatidyl inositol, lysophosphatidyl choline, lysophosphatidyl ethanolamine, phosphatidyl glycerol, and cardiolipin. No significant differences were noted in fatty acid composition.

BIOSYNTHESIS OF LIPIDS IN PLANTS: I. INCORPORATION OF ORTHOPHOSPHATE-32P AND GLYCEROPHOSPHATE-32P INTO PHOSPHATIDES OF CHLORELLA VULGARIS DURING PHOTOSYNTHESIS

1965 ◽  
Vol 43 (9) ◽  
pp. 1445-1453 ◽  
Author(s):  
P. S. Sastry ◽  
M. Kates
Keyword(s):  
Chlorella Vulgaris ◽  
Phosphatidyl Ethanolamine ◽  
Phosphatidyl Inositol ◽  
Biosynthetic Pathways ◽  
Bacterial Cells ◽  
Phosphatidyl Glycerol ◽  
Kinetics Of

The kinetics of incorporation of 32P from orthophosphate-32P or DL-α-glycerophosphate-32P into the phosphatides of Chlorella vulgaris during photosynthesis was studied. Rapid labelling of phosphatidyl glycerol was observed with both precursors, followed by lower rates of incorporation into lecithin, phosphatidyl ethanolamine, and phosphatidyl inositol. The results are discussed in the light of biosynthetic pathways known for animal and bacterial cells.

scholarly journals Screening Tests of Reproductive Immunology in Systemic Lupus Erythematosus

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Zdenka Ulcova-Gallova ◽  
Alice Mockova ◽  
Miroslava Cedikova
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Phosphatidyl Ethanolamine ◽  
Phosphatidyl Inositol ◽  
Phosphatidyl Serine ◽  
Sperm Cells ◽  
Systemic Lupus ◽  
Ethanolamine Phosphatidyl ◽  
Glycoprotein I ◽  
Beta 2

Female patients in reproductive age with systemic lupus erythematosus and fertility complications together are observed by rheumatologists, gynecologists, and reproductive immunologists. The paper notes the presence of autoantibodies to zona pellucida, to phospholipids (phosphatidyl serine, phosphatidyl ethanolamine, phosphatidyl inositol, phosphatidyl glycerol, phosphatidic acid, annexin V, beta-2 glycoprotein I, and cardiolipin) and of isoantibodies to sperm cells. Isoantibodies to sperm cells are not significantly predominant, but autoimmunity is well expressed in IgG positivity against phosphatidyl inositol, phosphatidyl ethanolamine, phosphatidyl serine, cardiolipin, and beta-2 glycoprotein I, as well as antizona pellucida antibodies in IgG isotype. According to the levels of autoantibodies we have to choose preventive treatment to protect mother and her foetus.

METABOLISM OF EHRLICH ASCITES TUMOR IN VITRO: SUBCELLULAR DISTRIBUTION OF PHOSPHOLIPIDS NEWLY FORMED FROM14C-LABELED PRECURSORS

1966 ◽  
Vol 44 (11) ◽  
pp. 1461-1468 ◽  
Author(s):  
V. Donisch ◽  
R. J. Rossiter
Keyword(s):  
Microsomal Fraction ◽  
Phosphatidyl Ethanolamine ◽  
Lipid Fraction ◽  
Specific Radioactivity ◽  
Phosphatidyl Inositol ◽  
Phosphatidyl Serine ◽  
Ethanolamine Phosphatidyl ◽  
Ehrlich Ascites ◽  
Ethanolamine Plasmalogen

When Ehrlich ascites cells were incubated in a suitable medium containing choline-1,2-14C, ethanolamine-1,2-14C, L-serine-14C, or glycerol-1-14C, radioactivity was recovered from the lipid fraction. With choline-1,2-14C, radioactivity was incorporated into the three choline-containing phospholipids, lecithin, choline plasmalogen, and sphingomyelin. Radioactivity from ethanolamine-1,2-14C was incorporated into phosphatidyl ethanolamine, ethanolamine plasmalogen, choline plasmalogen, and lecithin. Radioactivity from L-serine-14C was incorporated into phosphatidyl serine, serine plasmalogen, and phosphatide acid, with lesser amounts into phosphatidyl ethanolamine, lecithin, ethanolamine plasmalogen, choline plasmalogen, and sphingomyelin. Radioactivity from glycerol-1-14C was incorporated into the glycerophosphatides, phosphatidic acid, lecithin, phosphatidyl ethanolamine, phosphatidyl serine, phosphatidyl inositol, and choline plasmalogen. Radioactivity from this precursor was also incorporated into sphingomyelin.In all instances, radioactivity was recovered from the phosphatides in the nuclear, mitochondrial, and microsomal fractions of the tumor. Usually, the specific radioactivity of the phosphatides in the microsomal fraction exceeded that in the other two subcellular fractions.

scholarly journals Platelet Phospholipid Changes in Atherosclerosis

1977 ◽  
Author(s):  
A. Z. Aktulga ◽  
O. N. Ulutin
Keyword(s):  
Fibrinolytic Activity ◽  
Phosphatidyl Ethanolamine ◽  
Total Phospholipid ◽  
Phosphatidyl Inositol ◽  
Phosphatidyl Serine ◽  
Platelet Phospholipid ◽  
Lipid Contents ◽  
Coagulation Mechanism ◽  
The Individual ◽  
Platelet Functions

The platelet phospholipids content and their per cent distribution in atherosclerotic cases and in thromboembolic cases related to atherosclerosis was investigated with respect to normals. In normals the total phospholipid phosphorus value was found to be 10.88±0.24μg/109 platelets whereas this value changed to 14.29±0.39μg/109 platelets in atherosclerosis. The individual phospholipids follow the decreasing order of phosphatidyl choline, phosphatidyl ethanolamine, sphingomyeline, phosphatidyl serine, phosphatidyl inositol. In atherosclerotic cases although no difference was observed in this order, there was an increase in the quantity of certain phospholipids. In these cases the coagulation mechanism, the fibrinolytic activity, the platelet functions, and the serum lipid contents were also investigated in comparison to the above findings.

Clotting Activity of Platelet Phospholipids in Rat and Man

1974 ◽  
Vol 32 (02/03) ◽  
pp. 382-390 ◽  
Author(s):  
P Gautheron ◽  
E Dumont ◽  
S Renaud
Keyword(s):  
Phosphatidyl Choline ◽  
Phosphatidyl Ethanolamine ◽  
Phosphatidyl Inositol ◽  
Phosphatidyl Serine ◽  
The Other ◽  
Active Fraction ◽  
Platelet Phospholipid ◽  
Experimental Conditions ◽  
Ethanolamine Phosphatidyl ◽  
Recalcification Time

SummaryThe clotting activity of man and rat platelet phospholipid fractions separated by bi-dimensional TLC, resuspended in Tyrode by sonication, was studied in the recalcification (manual) and in the Stypven (recalcification plus Russell’s viper venom) clotting time (determined in a coagulometer). Phosphatidyl serine was the most active fraction to shorten the two clotting tests utilized, in both rat and man, but it was much more effective in the Stypven time. The phosphatidyl ethanolamine was the second most active fraction, in the Stypven time; this fraction was almost as active as phosphatidyl serine in both animal species. The other fractions studied (phosphatidyl inositol, phosphatidyl choline and sphingomyelin) were sligthly active or not active, depending on the experimental conditions.The clotting activity of platelet phosphatidyl serine from rat, at concentrations corresponding to platelet counts from 1 to 10 ( X105), was much smaller than this of the disrupted (sonicated) platelets from which it originated. However, the clotting activity of sonicated platelets could be completely reproduced, either at each concentration studied (Stypven time) or at a concentration corresponding to lOxlO5 platelets (recalcification time), by adding to phosphatidyl serine the other four phospholipid fractions (phosphatidyl inositol, phosphatidyl ethanolamine, phosphatidyl choline, sphingomyelin) dispersed in a homogeneous way by sonication.The feeding of a butter-rich diet to rats considerably increased the activity of each of the platelet phospholipid fractions in the two clotting tests carried out.

LIPIDS OF SERRATIA MARCESCENS

1964 ◽  
Vol 42 (4) ◽  
pp. 461-479 ◽  
Author(s):  
M. Kates ◽  
G. A. Adams ◽  
S. M. Martin
Keyword(s):  
Serratia Marcescens ◽  
Phosphatidyl Ethanolamine ◽  
Dry Weight ◽  
Phosphatidyl Serine ◽  
Monoenoic Acid ◽  
Phosphatidyl Glycerol ◽  
Active Growth ◽  
Stages Of Growth ◽  
Bound Lipids ◽  
Cellular Lipids

Cells of Serratia marcescens, whether pigmented or unpigmented, contained 10–11% of methanol–chloroform extractable lipids (dry weight basis) and < 1% of bound lipids. The extractable lipids contained 34–43% phosphatides, 3–11% unsaponifiable material, and 2–5% free fatty acid. The phosphatides contained high proportions of phosphatidyl ethanolamine and smaller amounts of phosphatidyl serine, polyglycerol phosphatides, phosphatidyl glycerol, and an unidentified ninhydrin-positive phosphatide probably associated with ornithine and other amino acids found in the lipid hydrolyzate.The fatty acids were found to consist largely of palmitic, C17- and C19-cyclopropane and C16- and C18-monoenoic acids. The proportions of monoenoic and cyclopropane acids were found to vary greatly with the age of the cultures; in the early stages of growth, regardless of pigmentation, low amounts of cyclopropane acids and high amounts of monoenoic acid were present, the latter being converted almost completely to cyclopropane acids during the active growth phase.The lipids associated with extracellular lipopolysaccharide material were similar in composition to the cellular lipids.

FORMATION OF PHOSPHATIDES FROM C14-LABELLED PRECURSORS IN RAT BRAIN SLICES. EFFECT OF CHLORPROMAZINE

1963 ◽  
Vol 41 (1) ◽  
pp. 341-345 ◽  
Author(s):  
E. T. Pritchard ◽  
R. J. Rossiter
Keyword(s):  
Guinea Pig ◽  
Phosphatidic Acid ◽  
Rat Brain ◽  
Phosphatidyl Ethanolamine ◽  
Brain Slices ◽  
Phosphatidyl Inositol ◽  
Phosphatidyl Serine ◽  
Inorganic P ◽  
The Individual ◽  
Guinea Pig Brain

The addition of chlorpromazine (0.1 mM) to slices of rat brain respiring in a suitable medium caused an increase in the incorporation of radioactivity from glycerol-1-C14, glycine-2-C14, and serine-3-C14 into the phospholipids of the slices. There was no increase in the incorporation of radioactivity from choline-1,2-C14 or ethanolamine-1,2-C14. Examination of the individual phosphatides showed an increase in the incorporation of radioactivity from glycerol-1-C14 into phosphatidc acid and phosphatidyl serine, with no change for lecithin and phosphatidyl ethanolamine. Higher concentrations of chlorpromazine (1.0 mM) either inhibited (glycerol-1-C14, choline-1,2-C14), did not significantly alter (glycine-2-C14, ethanolamine-1,2-C14), or stimulated (serine-3-C14) the incorporation of radioactivity into phospholipids. These results are discussed in relation to previous experiments, in which it was found that the addition of chlorpromazine (0.1 mM) to slices of guinea pig brain caused an increase in the incorporation of inorganic P32 into phosphatidic acid, phosphatidyl inositol, phosphatidyl serine, but not into lecithin or phosphatidyl ethanolamine.

Effects of Certain Phospholipids on Plasminogen Activation and Plasmin Activity

1962 ◽  
Vol 07 (01) ◽  
pp. 016-026
Author(s):  
Claire Gerbeck ◽  
J. L Koppel ◽  
John H Olwin
Keyword(s):  
Esterase Activity ◽  
Fibrinolytic Activity ◽  
Phosphatidyl Ethanolamine ◽  
Phosphatidyl Inositol ◽  
Phosphatidyl Serine ◽  
Optimum Level ◽  
Plasminogen Activation ◽  
Plasmin Activity ◽  
Caseinolytic Activity ◽  
Human Plasminogen

SummaryCrude and partially purified phospholipid preparations have been studied for their effects on a) the activation of purified human plasminogen by streptokinase and b) the activity of plasmin resulting from such activation. Plasmin activity has been measured as BAMe esterase, caseinolytic, and fibrinolytic activities.Plasmin BAMe esterase activity was significantly enhanced by phosphatidyl inositol and phosphatidyl serine but only slightly by phosphatidyl ethanolamine. Preparations of asolectin and cephalin, although not having any effect on plasmin BAMe esterase activity per se, did appear to increase plasminogen activation by streptokinase. None of the above-mentioned effects was observed when plasmin caseinolytic activity was measured. The phospholipid preparation having the greatest effect on plasmin BAMe esterase activity, phosphatidyl inositol, also exhibited an effect on plasmin fibrinolytic activity. With both BAMe esterase and fibrinolytic activities the effects produced by the phospholipid materials were concentration dependent and, in the case of fibrinolytic activity, increased concentrations beyond the optimum level resulted in a gradual diminution in activity and eventual inhibition.

FORMATION OF PHOSPHATIDES FROM C14-LABELLED PRECURSORS IN RAT BRAIN SLICES. EFFECT OF CHLORPROMAZINE

1963 ◽  
Vol 41 (2) ◽  
pp. 341-345 ◽  
Author(s):  
E. T. Pritchard ◽  
R. J. Rossiter
Keyword(s):  
Guinea Pig ◽  
Phosphatidic Acid ◽  
Rat Brain ◽  
Phosphatidyl Ethanolamine ◽  
Brain Slices ◽  
Phosphatidyl Inositol ◽  
Phosphatidyl Serine ◽  
Inorganic P ◽  
The Individual ◽  
Guinea Pig Brain

The addition of chlorpromazine (0.1 mM) to slices of rat brain respiring in a suitable medium caused an increase in the incorporation of radioactivity from glycerol-1-C14, glycine-2-C14, and serine-3-C14 into the phospholipids of the slices. There was no increase in the incorporation of radioactivity from choline-1,2-C14 or ethanolamine-1,2-C14. Examination of the individual phosphatides showed an increase in the incorporation of radioactivity from glycerol-1-C14 into phosphatidc acid and phosphatidyl serine, with no change for lecithin and phosphatidyl ethanolamine. Higher concentrations of chlorpromazine (1.0 mM) either inhibited (glycerol-1-C14, choline-1,2-C14), did not significantly alter (glycine-2-C14, ethanolamine-1,2-C14), or stimulated (serine-3-C14) the incorporation of radioactivity into phospholipids. These results are discussed in relation to previous experiments, in which it was found that the addition of chlorpromazine (0.1 mM) to slices of guinea pig brain caused an increase in the incorporation of inorganic P32 into phosphatidic acid, phosphatidyl inositol, phosphatidyl serine, but not into lecithin or phosphatidyl ethanolamine.

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