scholarly journals Platelet Phospholipid Changes in Atherosclerosis

1977 ◽  
Author(s):  
A. Z. Aktulga ◽  
O. N. Ulutin
Keyword(s):  
Fibrinolytic Activity ◽  
Phosphatidyl Ethanolamine ◽  
Total Phospholipid ◽  
Phosphatidyl Inositol ◽  
Phosphatidyl Serine ◽  
Platelet Phospholipid ◽  
Lipid Contents ◽  
Coagulation Mechanism ◽  
The Individual ◽  
Platelet Functions

The platelet phospholipids content and their per cent distribution in atherosclerotic cases and in thromboembolic cases related to atherosclerosis was investigated with respect to normals. In normals the total phospholipid phosphorus value was found to be 10.88±0.24μg/109 platelets whereas this value changed to 14.29±0.39μg/109 platelets in atherosclerosis. The individual phospholipids follow the decreasing order of phosphatidyl choline, phosphatidyl ethanolamine, sphingomyeline, phosphatidyl serine, phosphatidyl inositol. In atherosclerotic cases although no difference was observed in this order, there was an increase in the quantity of certain phospholipids. In these cases the coagulation mechanism, the fibrinolytic activity, the platelet functions, and the serum lipid contents were also investigated in comparison to the above findings.

Platelet Functions in Relation to Dietary Fats in Farmers from two Regions of France

1978 ◽  
Vol 40 (02) ◽  
pp. 518-531 ◽  
Author(s):  
S Renaud ◽  
E Dumont ◽  
F Godsey ◽  
A Suplisson ◽  
C Thevenon
Keyword(s):  
Platelet Aggregation ◽  
Platelet Rich Plasma ◽  
Plasma Cholesterol ◽  
Phospholipid Composition ◽  
Saturated Fat ◽  
Phosphatidyl Inositol ◽  
Phosphatidyl Serine ◽  
Dietary Fats ◽  
Platelet Phospholipid ◽  
Platelet Functions

SummaryTo determine whether the long-term feeding of dietary fats affect platelet functions in man, platelet aggregation (to thrombin ADP, collagen, epinephrine) and clotting activity of platelet-rich plasma (PRP), platelet-poor plasma and of washed platelets, were studied in a mobile-laboratory in 44 healthy male farmers (40-45 years) from two French regions Var and Moselle, in relation to lipemia, glycemia, dietary nutriments, and platelet phospholipid composition. In the Moselle subjects, the platelet clotting activity of PRP and of washed platelets, the platelet aggregation to thrombin and ADP, were highly significantly (p <0.001) increased as compared to those of Var, but not the plasma cholesterol, which was identical in the two regions.In Moselle, the intake of total calories, total lipids and saturated fats was higher than in the Var.However, it was only with the saturated fat intake (mostly stearic acid) that the platelet clotting activity (p <0.01) and the platelet aggregation (p <0.001) were highly significantly correlated. The platelet clotting activity was also significantly (p <0.001) correlated with the fatty acid composition of the platelet phospholipid fractions phosphatidyl serine + phosphatidyl inositol.

Clotting Activity of Platelet Phospholipids in Rat and Man

1974 ◽  
Vol 32 (02/03) ◽  
pp. 382-390 ◽  
Author(s):  
P Gautheron ◽  
E Dumont ◽  
S Renaud
Keyword(s):  
Phosphatidyl Choline ◽  
Phosphatidyl Ethanolamine ◽  
Phosphatidyl Inositol ◽  
Phosphatidyl Serine ◽  
The Other ◽  
Active Fraction ◽  
Platelet Phospholipid ◽  
Experimental Conditions ◽  
Ethanolamine Phosphatidyl ◽  
Recalcification Time

SummaryThe clotting activity of man and rat platelet phospholipid fractions separated by bi-dimensional TLC, resuspended in Tyrode by sonication, was studied in the recalcification (manual) and in the Stypven (recalcification plus Russell’s viper venom) clotting time (determined in a coagulometer). Phosphatidyl serine was the most active fraction to shorten the two clotting tests utilized, in both rat and man, but it was much more effective in the Stypven time. The phosphatidyl ethanolamine was the second most active fraction, in the Stypven time; this fraction was almost as active as phosphatidyl serine in both animal species. The other fractions studied (phosphatidyl inositol, phosphatidyl choline and sphingomyelin) were sligthly active or not active, depending on the experimental conditions.The clotting activity of platelet phosphatidyl serine from rat, at concentrations corresponding to platelet counts from 1 to 10 ( X105), was much smaller than this of the disrupted (sonicated) platelets from which it originated. However, the clotting activity of sonicated platelets could be completely reproduced, either at each concentration studied (Stypven time) or at a concentration corresponding to lOxlO5 platelets (recalcification time), by adding to phosphatidyl serine the other four phospholipid fractions (phosphatidyl inositol, phosphatidyl ethanolamine, phosphatidyl choline, sphingomyelin) dispersed in a homogeneous way by sonication.The feeding of a butter-rich diet to rats considerably increased the activity of each of the platelet phospholipid fractions in the two clotting tests carried out.

FORMATION OF PHOSPHATIDES FROM C14-LABELLED PRECURSORS IN RAT BRAIN SLICES. EFFECT OF CHLORPROMAZINE

1963 ◽  
Vol 41 (1) ◽  
pp. 341-345 ◽  
Author(s):  
E. T. Pritchard ◽  
R. J. Rossiter
Keyword(s):  
Guinea Pig ◽  
Phosphatidic Acid ◽  
Rat Brain ◽  
Phosphatidyl Ethanolamine ◽  
Brain Slices ◽  
Phosphatidyl Inositol ◽  
Phosphatidyl Serine ◽  
Inorganic P ◽  
The Individual ◽  
Guinea Pig Brain

The addition of chlorpromazine (0.1 mM) to slices of rat brain respiring in a suitable medium caused an increase in the incorporation of radioactivity from glycerol-1-C14, glycine-2-C14, and serine-3-C14 into the phospholipids of the slices. There was no increase in the incorporation of radioactivity from choline-1,2-C14 or ethanolamine-1,2-C14. Examination of the individual phosphatides showed an increase in the incorporation of radioactivity from glycerol-1-C14 into phosphatidc acid and phosphatidyl serine, with no change for lecithin and phosphatidyl ethanolamine. Higher concentrations of chlorpromazine (1.0 mM) either inhibited (glycerol-1-C14, choline-1,2-C14), did not significantly alter (glycine-2-C14, ethanolamine-1,2-C14), or stimulated (serine-3-C14) the incorporation of radioactivity into phospholipids. These results are discussed in relation to previous experiments, in which it was found that the addition of chlorpromazine (0.1 mM) to slices of guinea pig brain caused an increase in the incorporation of inorganic P32 into phosphatidic acid, phosphatidyl inositol, phosphatidyl serine, but not into lecithin or phosphatidyl ethanolamine.

Effects of Certain Phospholipids on Plasminogen Activation and Plasmin Activity

1962 ◽  
Vol 07 (01) ◽  
pp. 016-026
Author(s):  
Claire Gerbeck ◽  
J. L Koppel ◽  
John H Olwin
Keyword(s):  
Esterase Activity ◽  
Fibrinolytic Activity ◽  
Phosphatidyl Ethanolamine ◽  
Phosphatidyl Inositol ◽  
Phosphatidyl Serine ◽  
Optimum Level ◽  
Plasminogen Activation ◽  
Plasmin Activity ◽  
Caseinolytic Activity ◽  
Human Plasminogen

SummaryCrude and partially purified phospholipid preparations have been studied for their effects on a) the activation of purified human plasminogen by streptokinase and b) the activity of plasmin resulting from such activation. Plasmin activity has been measured as BAMe esterase, caseinolytic, and fibrinolytic activities.Plasmin BAMe esterase activity was significantly enhanced by phosphatidyl inositol and phosphatidyl serine but only slightly by phosphatidyl ethanolamine. Preparations of asolectin and cephalin, although not having any effect on plasmin BAMe esterase activity per se, did appear to increase plasminogen activation by streptokinase. None of the above-mentioned effects was observed when plasmin caseinolytic activity was measured. The phospholipid preparation having the greatest effect on plasmin BAMe esterase activity, phosphatidyl inositol, also exhibited an effect on plasmin fibrinolytic activity. With both BAMe esterase and fibrinolytic activities the effects produced by the phospholipid materials were concentration dependent and, in the case of fibrinolytic activity, increased concentrations beyond the optimum level resulted in a gradual diminution in activity and eventual inhibition.

FORMATION OF PHOSPHATIDES FROM C14-LABELLED PRECURSORS IN RAT BRAIN SLICES. EFFECT OF CHLORPROMAZINE

1963 ◽  
Vol 41 (2) ◽  
pp. 341-345 ◽  
Author(s):  
E. T. Pritchard ◽  
R. J. Rossiter
Keyword(s):  
Guinea Pig ◽  
Phosphatidic Acid ◽  
Rat Brain ◽  
Phosphatidyl Ethanolamine ◽  
Brain Slices ◽  
Phosphatidyl Inositol ◽  
Phosphatidyl Serine ◽  
Inorganic P ◽  
The Individual ◽  
Guinea Pig Brain

The addition of chlorpromazine (0.1 mM) to slices of rat brain respiring in a suitable medium caused an increase in the incorporation of radioactivity from glycerol-1-C14, glycine-2-C14, and serine-3-C14 into the phospholipids of the slices. There was no increase in the incorporation of radioactivity from choline-1,2-C14 or ethanolamine-1,2-C14. Examination of the individual phosphatides showed an increase in the incorporation of radioactivity from glycerol-1-C14 into phosphatidc acid and phosphatidyl serine, with no change for lecithin and phosphatidyl ethanolamine. Higher concentrations of chlorpromazine (1.0 mM) either inhibited (glycerol-1-C14, choline-1,2-C14), did not significantly alter (glycine-2-C14, ethanolamine-1,2-C14), or stimulated (serine-3-C14) the incorporation of radioactivity into phospholipids. These results are discussed in relation to previous experiments, in which it was found that the addition of chlorpromazine (0.1 mM) to slices of guinea pig brain caused an increase in the incorporation of inorganic P32 into phosphatidic acid, phosphatidyl inositol, phosphatidyl serine, but not into lecithin or phosphatidyl ethanolamine.

scholarly journals Screening Tests of Reproductive Immunology in Systemic Lupus Erythematosus

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Zdenka Ulcova-Gallova ◽  
Alice Mockova ◽  
Miroslava Cedikova
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Phosphatidyl Ethanolamine ◽  
Phosphatidyl Inositol ◽  
Phosphatidyl Serine ◽  
Sperm Cells ◽  
Systemic Lupus ◽  
Ethanolamine Phosphatidyl ◽  
Glycoprotein I ◽  
Beta 2

Female patients in reproductive age with systemic lupus erythematosus and fertility complications together are observed by rheumatologists, gynecologists, and reproductive immunologists. The paper notes the presence of autoantibodies to zona pellucida, to phospholipids (phosphatidyl serine, phosphatidyl ethanolamine, phosphatidyl inositol, phosphatidyl glycerol, phosphatidic acid, annexin V, beta-2 glycoprotein I, and cardiolipin) and of isoantibodies to sperm cells. Isoantibodies to sperm cells are not significantly predominant, but autoimmunity is well expressed in IgG positivity against phosphatidyl inositol, phosphatidyl ethanolamine, phosphatidyl serine, cardiolipin, and beta-2 glycoprotein I, as well as antizona pellucida antibodies in IgG isotype. According to the levels of autoantibodies we have to choose preventive treatment to protect mother and her foetus.

METABOLISM OF EHRLICH ASCITES TUMOR IN VITRO: SUBCELLULAR DISTRIBUTION OF PHOSPHOLIPIDS NEWLY FORMED FROM14C-LABELED PRECURSORS

1966 ◽  
Vol 44 (11) ◽  
pp. 1461-1468 ◽  
Author(s):  
V. Donisch ◽  
R. J. Rossiter
Keyword(s):  
Microsomal Fraction ◽  
Phosphatidyl Ethanolamine ◽  
Lipid Fraction ◽  
Specific Radioactivity ◽  
Phosphatidyl Inositol ◽  
Phosphatidyl Serine ◽  
Ethanolamine Phosphatidyl ◽  
Ehrlich Ascites ◽  
Ethanolamine Plasmalogen

When Ehrlich ascites cells were incubated in a suitable medium containing choline-1,2-14C, ethanolamine-1,2-14C, L-serine-14C, or glycerol-1-14C, radioactivity was recovered from the lipid fraction. With choline-1,2-14C, radioactivity was incorporated into the three choline-containing phospholipids, lecithin, choline plasmalogen, and sphingomyelin. Radioactivity from ethanolamine-1,2-14C was incorporated into phosphatidyl ethanolamine, ethanolamine plasmalogen, choline plasmalogen, and lecithin. Radioactivity from L-serine-14C was incorporated into phosphatidyl serine, serine plasmalogen, and phosphatide acid, with lesser amounts into phosphatidyl ethanolamine, lecithin, ethanolamine plasmalogen, choline plasmalogen, and sphingomyelin. Radioactivity from glycerol-1-14C was incorporated into the glycerophosphatides, phosphatidic acid, lecithin, phosphatidyl ethanolamine, phosphatidyl serine, phosphatidyl inositol, and choline plasmalogen. Radioactivity from this precursor was also incorporated into sphingomyelin.In all instances, radioactivity was recovered from the phosphatides in the nuclear, mitochondrial, and microsomal fractions of the tumor. Usually, the specific radioactivity of the phosphatides in the microsomal fraction exceeded that in the other two subcellular fractions.

THE LIVER PHOSPHOLIPIDS OF THE DEVELOPING CHICK EMBRYO

1967 ◽  
Vol 45 (5) ◽  
pp. 627-639 ◽  
Author(s):  
R. C. Noble ◽  
J. H. Moore
Keyword(s):  
Fatty Acid ◽  
Phosphatidyl Choline ◽  
Phosphatidyl Ethanolamine ◽  
Phosphatidyl Serine ◽  
Chick Embryos ◽  
Ethanolamine Phosphatidyl ◽  
The Individual ◽  
Docosahexaenoic Acids ◽  
Choline Phosphatidyl ◽  
Fatty Acid Compositions

The concentrations and fatty acid compositions of the individual phospholipids in the livers of chick embryos on the 13th, 15th, 17th, 19th, and 21st days of incubation were compared with the concentrations and fatty acid compositions of the individual yolk phospholipids. The liver phospholipids contained higher proportions of phosphatidyl ethanolamine, phosphatidyl serine, and diphosphatidyl glycerol, and lower proportions of phosphatidyl choline, than did the yolk phospholipids. There was a constant increment (0.96 mg/day) of phosphatidyl choline in the liver during the period of incubation studied. The phosphatidyl choline, phosphatidyl ethanolamine, and phosphatidyl serine of the liver generally contained higher concentrations of stearic, arachidonic, and docosahexaenoic acids, and lower concentrations of palmitic and oleic acids than did these phospholipid fractions in the yolk. The fatty acid compositions of the sphingomyelin in the liver and yolk were similar. The most pronounced changes in the fatty acid composition of the liver phospholipids during embryonic development were observed in the phosphatidyl choline fraction. These changes suggested that the α-palmitoyl-β-arachidonyl phosphatidyl choline in the liver was gradually replaced by α-stearoyl-β-linoleoyl phosphatidyl choline and a-stearoyl-β-docosahexaenoyl phosphatidyl choline.

scholarly journals Lipidomic profiling of the developing kernel clarifies the lipid metabolism of Paeonia ostii

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Shui-Yan Yu ◽  
Ying Zhang ◽  
Yu-Ping Lyu ◽  
Zu-Jie Yao ◽  
Yong-Hong Hu
Keyword(s):  
Phosphatidyl Ethanolamine ◽  
Phosphatidyl Inositol ◽  
Phosphatidyl Serine ◽  
Sphingolipid Metabolism ◽  
Phosphatidyl Glycerol ◽  
Alpha Linolenic Acid ◽  
Oil Accumulation ◽  
Oil Crops ◽  
Sphingosine 1 Phosphate ◽  
Fatty Acyls

AbstractLipid components in the developing kernel of Paeonia ostii were determined, and the fatty acid (FA) distributions in triacylglycerol and phospholipids were characterized. The lipids in the kernel were mainly phospholipids (43%), neutral glycerides (24%), fatty acyls (26%), and sphingolipids (4.5%). The dominant neutral glycerides were TAG and diacylglycerol. The PL components included phosphatidic acid, phosphatidyl glycerol, phosphatidyl choline, phosphatidyl serine, phosphatidyl inositol, and phosphatidyl ethanolamine. As the kernel developed, the profiles of the molecular species comprising TAG and PL changed, especially during the earlier phases of oil accumulation. During rapid oil accumulation, the abundances of sphingosine-1-phosphate, pyruvic acid, stearic acid, and alpha-linolenic acid changed significantly; the sphingolipid metabolism and unsaturated FAs biosynthesis pathways were significantly enriched in these differentially abundant metabolites. Our results improve our understanding of lipid accumulation in tree peony seeds, and provide a framework for the analysis of lipid metabolisms in other oil crops.

Effects of cholesterol on growth and lipid composition of Pythium sp. PRL 2142

1972 ◽  
Vol 18 (7) ◽  
pp. 1059-1063 ◽  
Author(s):  
J. A. Brushaber ◽  
J. J. Child ◽  
R. H. Haskins
Keyword(s):  
Growth Rate ◽  
Phosphatidyl Ethanolamine ◽  
Initial Period ◽  
Lipid Synthesis ◽  
Phosphatidyl Inositol ◽  
Dry Weight ◽  
Phosphatidyl Serine ◽  
Phosphatidyl Glycerol ◽  
Ethanolamine Phosphatidyl ◽  
Weight Yield

Addition of exogenous cholesterol to Pythium initially increases the growth rate, but the final dry weight yield is reduced. Cholesterol induces an overall increase in lipid synthesis after the initial period of rapid growth. The lipid content of cholesterol-grown mycelium becomes about double that of mycelium grown without cholesterol. The proportion of phosphatidyl serine relative to other phospholipids is reduced by half in mycelia grown with cholesterol. The major phospholipids are phosphatidyl ethanolamine, phosphatidyl serine, and phosphatidyl choline. Minor phospholipids identified are phosphatidyl inositol, lysophosphatidyl choline, lysophosphatidyl ethanolamine, phosphatidyl glycerol, and cardiolipin. No significant differences were noted in fatty acid composition.

Sign in / Sign up

Export Citation Format

Share Document