scholarly journals Experimental long-term diabetes mellitus alters the transcriptome and biomechanical properties of the rat urinary bladder

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Emad A. Hindi ◽  
Craig J. Williams ◽  
Leo A. H. Zeef ◽  
Filipa M. Lopes ◽  
Katie Newman ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Extracellular Matrix ◽  
Urinary Bladder ◽  
Biomechanical Properties ◽  
Molecular Signature ◽  
Detrusor Muscle ◽  
Rat Urinary Bladder ◽  
Force Microscopy ◽  
Diabetic Cystopathy

AbstractDiabetes mellitus (DM) is the leading cause of chronic kidney disease and diabetic nephropathy is widely studied. In contrast, the pathobiology of diabetic urinary bladder disease is less understood despite dysfunctional voiding being common in DM. We hypothesised that diabetic cystopathy has a characteristic molecular signature. We therefore studied bladders of hyperglycaemic and polyuric rats with streptozotocin (STZ)-induced DM. Sixteen weeks after induction of DM, as assessed by RNA arrays, wide-ranging changes of gene expression occurred in DM bladders over and above those induced in bladders of non-hyperglycaemic rats with sucrose-induced polyuria. The altered transcripts included those coding for extracellular matrix regulators and neural molecules. Changes in key genes deregulated in DM rat bladders were also detected in db/db mouse bladders. In DM rat bladders there was reduced birefringent collagen between detrusor muscle bundles, and atomic force microscopy showed a significant reduction in tissue stiffness; neither change was found in bladders of sucrose-treated rats. Thus, altered extracellular matrix with reduced tissue rigidity may contribute to voiding dysfunction in people with long-term DM. These results serve as an informative stepping stone towards understanding the complex pathobiology of diabetic cystopathy.

Optimization of a decellularization protocol of porcine tracheas. Long-term effects of cryopreservation. A histological study

2021 ◽  
pp. 039139882110089
Author(s):  
Lara Milian ◽  
María Sancho-Tello ◽  
Joan Roig-Soriano ◽  
Giovanna Foschini ◽  
Néstor J Martínez-Hernández ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Histological Study ◽  
Biomechanical Properties ◽  
Elastic Fibers ◽  
Appreciable Effect ◽  
Long Term Effects ◽  
Minimal Impact ◽  
Biomechanical Effect ◽  
Triton X

Objective: The aim of this study was to optimize a decellularization protocol in the trachea of Sus scrofa domestica (pig) as well as to study the effects of long-term cryopreservation on the extracellular matrix of decellularized tracheas. Methods: Porcine tracheas were decellularized using Triton X-100, SDC, and SDS alone or in combination. The effect of these detergents on the extracellular matrix characteristics of decellularized porcine tracheas was evaluated at the histological, biomechanical, and biocompatibility level. Morphometric approaches were used to estimate the effect of detergents on the collagen and elastic fibers content as well as on the removal of chondrocytes from decellularized organs. Moreover, the long-term structural, ultrastructural, and biomechanical effect of cryopreservation of decellularized tracheas were also estimated. Results: Two percent SDS was the most effective detergent tested concerning cell removal and preservation of the histological and biomechanical properties of the tracheal wall. However, long-term cryopreservation had no an appreciable effect on the structure, ultrastructure, and biomechanics of decellularized tracheal rings. Conclusion: The results presented here reinforce the use of SDS as a valuable decellularizing agent for porcine tracheas. Furthermore, a cryogenic preservation protocol is described, which has minimal impact on the histological and biomechanical properties of decellularized porcine tracheas.

The influence of diabetes mellitus on glucose utilization by the rat urinary bladder

Metabolism ◽  
1993 ◽  
Vol 42 (6) ◽  
pp. 749-755 ◽  
Author(s):  
Penelope A. Longhurst ◽  
Janice A.K. Briscoe ◽  
Robert E. Leggett ◽  
Saeed Samadzadeh ◽  
Robert M. Levin
Keyword(s):  
Diabetes Mellitus ◽  
Urinary Bladder ◽  
Glucose Utilization ◽  
Rat Urinary Bladder

Characterization of αGal(−) ECM Bioscaffolds: An Indication for Application in Tissue Engineering

2011 ◽  
Author(s):  
Rui Liang ◽  
Matthew B. Fisher ◽  
Guoguang Yang ◽  
Christine Hall ◽  
Savio L-Y. Woo
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Small Intestine ◽  
Animal Models ◽  
Urinary Bladder ◽  
Biomechanical Properties ◽  
Small Intestine Submucosa ◽  
Urinary Bladder Matrix ◽  
Porcine Small Intestine Submucosa

Extracellular matrix (ECM) bioscaffolds derived from animal sources, such as porcine small intestine submucosa (SIS) and urinary bladder matrix (UBM), have a successful history for application in clinical and/or experimental settings [1]. In our research center, we have demonstrated that the porcine SIS bioscaffolds could improve the morphological, biochemical, and biomechanical properties of healing ligaments and tendons in different animal models [2, 3].

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