Optimization of a decellularization protocol of porcine tracheas. Long-term effects of cryopreservation. A histological study

Objective: The aim of this study was to optimize a decellularization protocol in the trachea of Sus scrofa domestica (pig) as well as to study the effects of long-term cryopreservation on the extracellular matrix of decellularized tracheas. Methods: Porcine tracheas were decellularized using Triton X-100, SDC, and SDS alone or in combination. The effect of these detergents on the extracellular matrix characteristics of decellularized porcine tracheas was evaluated at the histological, biomechanical, and biocompatibility level. Morphometric approaches were used to estimate the effect of detergents on the collagen and elastic fibers content as well as on the removal of chondrocytes from decellularized organs. Moreover, the long-term structural, ultrastructural, and biomechanical effect of cryopreservation of decellularized tracheas were also estimated. Results: Two percent SDS was the most effective detergent tested concerning cell removal and preservation of the histological and biomechanical properties of the tracheal wall. However, long-term cryopreservation had no an appreciable effect on the structure, ultrastructure, and biomechanics of decellularized tracheal rings. Conclusion: The results presented here reinforce the use of SDS as a valuable decellularizing agent for porcine tracheas. Furthermore, a cryogenic preservation protocol is described, which has minimal impact on the histological and biomechanical properties of decellularized porcine tracheas.

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Severe but not moderate hyperoxia of newborn mice causes an emphysematous lung phenotype in adulthood without persisting oxidative stress and inflammation

BMC Pulmonary Medicine ◽

10.1186/s12890-019-0993-5 ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 2

Author(s):

Anke Kindermann ◽

Leonore Binder ◽

Jan Baier ◽

Beate Gündel ◽

Andreas Simm ◽

...

Keyword(s):

Oxidative Stress ◽

Later Life ◽

Lung Compliance ◽

Elastic Fibers ◽

Newborn Mouse ◽

Long Term Effects ◽

Newborn Mice ◽

Functional Changes ◽

Oxygen Concentrations

Abstract Background Preterm newborns typically require supplemental oxygen but hyperoxic conditions also damage the premature lung. Oxygen-induced lung damages are mainly studied in newborn mouse models using oxygen concentrations above 75% and looking at short-term effects. Therefore, we aimed at the investigation of long-term effects and their dependency on different oxygen concentrations. Methods Newborn mice were exposed to moderate vs. severe hyperoxic air conditions (50 vs. 75% O2) for 14 days followed by a longer period of normoxic conditions. Lung-related parameters were collected at an age of 60 or 120 days. Results Severe hyperoxia caused lower alveolar density, enlargement of parenchymal air spaces and fragmented elastic fibers as well as higher lung compliance with peak airflow limitations and higher sensitivity to ventilation-mediated damages in later life. However, these long-term lung structural and functional changes did not restrict the voluntary physical activity. Also, they were not accompanied by ongoing inflammatory processes, increased formation of reactive oxygen species (ROS) or altered expressions of antioxidant enzymes (superoxide dismutases, catalase) and lung elasticity-relevant proteins (elastin, pro-surfactant proteins) in adulthood. In contrast to severe hyperoxia, moderate hyperoxia was less lung damaging but also not free of long-term effects (higher lung compliance without peak airflow limitations, increased ROS formation). Conclusions Severe but not moderate neonatal hyperoxia causes emphysematous lungs without persisting oxidative stress and inflammation in adulthood. As the existing fragmentation of the elastic fibers seems to play a pivotal role, it indicates the usefulness of elastin-protecting compounds in the reduction of long-term oxygen-related lung damages.

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234. Long Term Effects of pcDNA3_VEGF165 Intramyocardial Transfer after Experimental Myocardial Infarction in the Rat Scar Tissue Extracellular Matrix

Molecular Therapy ◽

10.1016/j.ymthe.2006.08.260 ◽

2006 ◽

Vol 13 ◽

pp. S90

Author(s):

Fabio D. Mataveli ◽

Sang W. Han ◽

Aline Mendes ◽

Rose Kanashiro ◽

Paulo Tucci ◽

...

Keyword(s):

Myocardial Infarction ◽

Extracellular Matrix ◽

Scar Tissue ◽

Experimental Myocardial Infarction ◽

Long Term Effects

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Histological study of long-term effects induced by systemic administration of KA at different developing ages

Neurochemistry International ◽

10.1016/0197-0186(90)90344-s ◽

1990 ◽

Vol 16 ◽

pp. 62

Keyword(s):

Histological Study ◽

Systemic Administration ◽

Long Term Effects

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Short- and long-term effects of small bowel resection: a unique histological study in a piglet model of short bowel syndrome

Histochemistry and Cell Biology ◽

10.1007/s00418-011-0778-2 ◽

2011 ◽

Vol 135 (2) ◽

pp. 195-202 ◽

Cited By ~ 24

Author(s):

Prue M. Pereira-Fantini ◽

Sarah L. Thomas ◽

Guineva Wilson ◽

Russell G. Taylor ◽

Magdy Sourial ◽

...

Keyword(s):

Small Bowel ◽

Short Bowel Syndrome ◽

Bowel Resection ◽

Histological Study ◽

Small Bowel Resection ◽

Long Term Effects ◽

Short Bowel ◽

Short And Long Term

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Primary Extracellular Matrix Enables Long-Term Cultivation of Human Tumor Oral Mucosa Models

Frontiers in Bioengineering and Biotechnology ◽

10.3389/fbioe.2020.579896 ◽

2020 ◽

Vol 8 ◽

Author(s):

Leonie Gronbach ◽

Philipp Jurmeister ◽

Monika Schäfer-Korting ◽

Ulrich Keilholz ◽

Ingeborg Tinhofer ◽

...

Keyword(s):

Extracellular Matrix ◽

Oral Mucosa ◽

Hypoxia Inducible Factor ◽

Human Tumor ◽

Patient Specific ◽

Long Term Effects ◽

Tumor Models

3D tumor models clearly outperform 2D cell cultures in recapitulating tissue architecture and drug response. However, their potential in understanding treatment efficacy and resistance development should be better exploited if also long-term effects of treatment could be assessed in vitro. The main disadvantages of the matrices commonly used for in vitro culture are their limited cultivation time and the low comparability with patient-specific matrix properties. Extended cultivation periods are feasible when primary human cells produce the extracellular matrix in situ. Herein, we adapted the hyalograft-3D approach from reconstructed human skin to normal and tumor oral mucosa models and compared the results to bovine collagen-based models. The hyalograft models showed similar morphology and cell proliferation after 7 weeks compared to collagen-based models after 2 weeks of cultivation. Tumor thickness and VEGF expression increased in hyalograft-based tumor models, whereas expression of laminin-332, tenascin C, and hypoxia-inducible factor 1α was lower than in collagen-based models. Taken together, the in situ produced extracellular matrix better confined tumor invasion in the first part of the cultivation period, with continuous tumor proliferation and increasing invasion later on. This proof-of-concept study showed the successful transfer of the hyalograft approach to tumor oral mucosa models and lays the foundation for the assessment of long-term drug treatment effects. Moreover, the use of an animal-derived extracellular matrix is avoided.

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Long-term effects for acute phase myocardial infarct VEGF165gene transfer cardiac extracellular matrix remodeling

Growth Factors ◽

10.1080/08977190802574765 ◽

2009 ◽

Vol 27 (1) ◽

pp. 22-31 ◽

Cited By ~ 10

Author(s):

Fabio D'Aguiar Mataveli ◽

Sang Won Han ◽

Helena Bonciani Nader ◽

Aline Mendes ◽

Rose Kanishiro ◽

...

Keyword(s):

Extracellular Matrix ◽

Acute Phase ◽

Myocardial Infarct ◽

Extracellular Matrix Remodeling ◽

Matrix Remodeling ◽

Long Term Effects

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Evaluation of long-term effects of artificial sweeteners on rat brain: a biochemical, behavioral, and histological study

Journal of Biochemical and Molecular Toxicology ◽

10.1002/jbt.22053 ◽

2018 ◽

Vol 32 (6) ◽

pp. e22053 ◽

Cited By ~ 6

Author(s):

Oytun Erbaş ◽

Mümin Alper Erdoğan ◽

Asghar Khalilnezhad ◽

Volkan Solmaz ◽

Fulya Tuzcu Gürkan ◽

...

Keyword(s):

Rat Brain ◽

Histological Study ◽

Artificial Sweeteners ◽

Long Term Effects

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Extracellular Matrix Determines Biomechanical Properties of Chondrospheres during Their Maturation In Vitro

Cartilage ◽

10.1177/1947603518798890 ◽

2018 ◽

Vol 11 (4) ◽

pp. 521-531 ◽

Cited By ~ 6

Author(s):

Nikolai P. Omelyanenko ◽

Pavel A. Karalkin ◽

Elena A. Bulanova ◽

Elizaveta V. Koudan ◽

Vladislav A. Parfenov ◽

...

Keyword(s):

Extracellular Matrix ◽

Self Assembly ◽

Biomechanical Properties ◽

Culture Conditions ◽

Secant Modulus ◽

Structural Determinants ◽

Maturation In Vitro

Objective Chondrospheres represent a variant of tissue spheroids biofabricated from chondrocytes. They are already being used in clinical trials for cartilage repair; however, their biomechanical properties have not been systematically investigated yet. The aim of our study was to characterize chondrospheres in long-term in vitro culture conditions for morphometric changes, biomechanical integrity, and their fusion and spreading kinetics. Results It has been demonstrated that the increase in chondrospheres secant modulus of elasticity is strongly associated with the synthesis and accumulation of extracellular matrix. Additionally, significant interplay has been found between biomechanical properties of tissue spheroids and their fusion kinetics in contrast to their spreading kinetics. Conclusions Extracellular matrix is one of the main structural determinants of chondrospheres biomechanical properties during chondrogenic maturation in vitro. The estimation of tissue spheroids’ physical behavior in vitro prior to operative treatment can be used to predict and potentially control fusogenic self-assembly process after implantation in vivo.

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Are There Long-Term Effects of the Vietnam Draft on Political Attitudes or Behavior? Apparently Not

Journal of Experimental Political Science ◽

10.1017/xps.2018.18 ◽

2018 ◽

Vol 6 (02) ◽

pp. 71-80 ◽

Cited By ~ 1

Author(s):

Donald P. Green ◽

Tiffany C. Davenport ◽

Kolby Hanson

Keyword(s):

Vietnam War ◽

Political Attitudes ◽

Economic Effects ◽

Appreciable Effect ◽

Long Term Effects ◽

Early Experiences ◽

Wide Range ◽

Draft Lottery ◽

Vietnam Draft

AbstractThe Vietnam draft lottery exposed millions of men to risk of induction at a time when the Vietnam War was becoming increasingly unpopular. We study the long-term effects of draft risk on political attitudes and behaviors of men who were eligible for the draft in 1969–1971. Our 2014–2016 surveys of men who were eligible for the Vietnam draft lotteries reveal no appreciable effect of draft risk across a wide range of political attitudes. These findings are bolstered by analysis of a vast voter registration database, which shows no differences in voting rates or tendency to register with the Democratic or Republican parties. The pattern of weak long-term effects is in line with studies showing that the long-term economic effects of Vietnam draft risk dissipated over time and offers a counterweight to influential observational studies that report long-term persistence in the effects of early experiences on political attitudes.

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