scholarly journals Dengue virus dominates lipid metabolism modulations in Wolbachia-coinfected Aedes aegypti

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Cassandra Koh ◽  
M. Nurul Islam ◽  
Yixin H. Ye ◽  
Nunya Chotiwan ◽  
Barbara Graham ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Molecular Level ◽  
Lipid Profiles ◽  
Virus Serotype ◽  
Direct Competition ◽  
New Research ◽  
Virus Interactions ◽  
Virus Blocking ◽  
Dual Infections

AbstractCompetition between viruses and Wolbachia for host lipids is a proposed mechanism of Wolbachia-mediated virus blocking in insects. Yet, the metabolomic interaction between virus and symbiont within the mosquito has not been clearly defined. We compare the lipid profiles of Aedes aegypti mosquitoes bearing mono- or dual-infections of the Wolbachia wMel strain and dengue virus serotype 3 (DENV3). We found metabolic signatures of infection-induced intracellular events but little evidence to support direct competition between Wolbachia and virus for host lipids. Lipid profiles of dual-infected mosquitoes resemble those of DENV3 mono-infected mosquitoes, suggesting virus-driven modulation dominates over that of Wolbachia. Interestingly, knockdown of key metabolic enzymes suggests cardiolipins are host factors for DENV3 and Wolbachia replication. These findings define the Wolbachia-DENV3 metabolic interaction as indirectly antagonistic, rather than directly competitive, and reveal new research avenues with respect to mosquito × virus interactions at the molecular level.

scholarly journals Selection on Aedes aegypti alters Wolbachia-mediated dengue virus blocking and fitness

2019 ◽  
Vol 4 (11) ◽  
pp. 1832-1839 ◽  
Author(s):  
Suzanne A. Ford ◽  
Scott L. Allen ◽  
Johanna R. Ohm ◽  
Leah T. Sigle ◽  
Aswathy Sebastian ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Virus Blocking

scholarly journals The Influence of Dengue Virus Serotype-2 Infection on Aedes aegypti (Diptera: Culicidae) Motivation and Avidity to Blood Feed

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e65252 ◽  
Author(s):  
Rafael Maciel-de-Freitas ◽  
Gabriel Sylvestre ◽  
Mariana Gandini ◽  
Jacob C. Koella
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Virus Serotype ◽  
Dengue Virus Serotype 2

scholarly journals Under-the-Radar Dengue Virus Infections in Natural Populations of Aedes aegypti Mosquitoes

mSphere ◽  
2020 ◽  
Vol 5 (2) ◽  
Author(s):  
Sean M. Boyles ◽  
Carla N. Mavian ◽  
Esteban Finol ◽  
Maria Ukhanova ◽  
Caroline J. Stephenson ◽  
...  
Keyword(s):  
United States ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
The United States ◽  
Human Case ◽  
Mosquito Vector ◽  
Public Health Response ◽  
Content Type ◽  
Virus Serotype ◽  
Dengue Outbreaks

ABSTRACT The incidence of locally acquired dengue infections increased during the last decade in the United States, compelling a sustained research effort concerning the dengue mosquito vector, Aedes aegypti, and its microbiome, which has been shown to influence virus transmission success. We examined the “metavirome” of four populations of Aedes aegypti mosquitoes collected in 2016 to 2017 in Manatee County, FL. Unexpectedly, we discovered that dengue virus serotype 4 (DENV4) was circulating in these mosquito populations, representing the first documented case of such a phenomenon in the absence of a local DENV4 human case in this county over a 2-year period. We confirmed that all of the mosquito populations carried the same DENV4 strain, assembled its full genome, validated infection orthogonally by reverse transcriptase PCR, traced the virus origin, estimated the time period of its introduction to the Caribbean region, and explored the viral genetic signatures and mosquito-specific virome associations that potentially mediated DENV4 persistence in mosquitoes. We discuss the significance of prolonged maintenance of the DENV4 infections in A. aegypti that occurred in the absence of a DENV4 human index case in Manatee County with respect to the inability of current surveillance paradigms to detect mosquito vector infections prior to a potential local outbreak. IMPORTANCE Since 1999, dengue outbreaks in the continental United States involving local transmission have occurred only episodically and only in Florida and Texas. In Florida, these episodes appear to be coincident with increased introductions of dengue virus into the region through human travel and migration from countries where the disease is endemic. To date, the U.S. public health response to dengue outbreaks has been largely reactive, and implementation of comprehensive arbovirus surveillance in advance of predictable transmission seasons, which would enable proactive preventative efforts, remains unsupported. The significance of our finding is that it is the first documented report of DENV4 transmission to and maintenance within a local mosquito vector population in the continental United States in the absence of a human case during two consecutive years. Our data suggest that molecular surveillance of mosquito populations in high-risk, high-tourism areas of the United States may enable proactive, targeted vector control before potential arbovirus outbreaks.

scholarly journals Immune transcript variations among Aedes aegypti populations with distinct susceptibility to dengue virus serotype 2

Acta Tropica ◽  
2012 ◽  
Vol 124 (2) ◽  
pp. 113-119 ◽  
Author(s):  
D. Carvalho-Leandro ◽  
C.F.J. Ayres ◽  
D.R.D. Guedes ◽  
L. Suesdek ◽  
M.A.V. Melo-Santos ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Virus Serotype ◽  
Dengue Virus Serotype 2

scholarly journals Resistance Status of Dengue Virus Serotype in Aedes Aegypti on The Exposure of Insecticide Temefos and Cypermethrin

2020 ◽  
Vol 5 (2) ◽  
pp. 215-220
Author(s):  
Arimaswati Arimaswati ◽  
La Ode Alifariki ◽  
Heriviyatno Julika Siagian ◽  
Tukatman Tukatman ◽  
La Rangki
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Virus Serotype

scholarly journals Intrinsic variation in the vertically transmitted insect-specific core virome of Aedes aegypti

2021 ◽  
Author(s):  
H. Coatsworth ◽  
J Bozic ◽  
J. Carrillo ◽  
E.A. Buckner ◽  
A.R. Rivers ◽  
...  
Keyword(s):  
United States ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Index Case ◽  
Natural Populations ◽  
The United States ◽  
Sample Type ◽  
Rt Pcr ◽  
Fundamental Information ◽  
Virus Serotype

AbstractSince 2009, local outbreaks of dengue (serotypes 1-3) mediated by Aedes aegypti mosquitoes have occurred in the United States, particularly in Florida (FL). In 2016 and 2017, dengue virus serotype 4 was found alongside several insect-specific viruses (ISVs) in pools of A. aegypti from sites in Manatee County, FL, in the absence of an index case. Although ISVs have been characterized in A. aegypti globally, the constitution of a core virome in natural populations remains unclear. Using mosquitoes sampled from the same area in 2018, we compared baseline ovary viromes of field (G0) and lab (Orlando) A. aegypti via metagenomic RNA sequencing. Across all samples, virome composition varied by sample type (field- or colony-derived). Four ISVs comprised >97% of virus sequences: a novel partiti-like virus (Partitiviridae), a previously described toti-like virus (Totiviridae), unclassified Riboviria, and four previously described orthomyxo-like viruses (Orthormyxoviridae). Whole or partial genomes for the toti-like virus, partiti-like virus, and one orthomyxo-like virus were assembled and analyzed phylogenetically. Multigenerational maintenance of these ISVs was confirmed orthogonally by RT-PCR in G0 and G7 mosquitoes, indicating vertical transmission as the mechanism for ISV sustentation. This study provides fundamental information regarding ISV ecology, persistence, and variation in A. aegypti in nature.

scholarly journals Family level variation in Wolbachia-mediated dengue virus blocking in Aedes aegypti

2017 ◽  
Vol 10 (1) ◽  
Author(s):  
Gerard Terradas ◽  
Scott L. Allen ◽  
Stephen F. Chenoweth ◽  
Elizabeth A. McGraw
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Level Variation ◽  
Family Level ◽  
Virus Blocking

scholarly journals Infection Kinetics and Transmissibility of a Reanimated Dengue Virus Serotype 4 Identified Originally in Wild Aedes aegypti From Florida

2021 ◽  
Vol 12 ◽  
Author(s):  
Jasmine B. Ayers ◽  
Xuping Xie ◽  
Heather Coatsworth ◽  
Caroline J. Stephenson ◽  
Christy M. Waits ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Adult Female ◽  
Disease Risk ◽  
Homo Sapiens ◽  
Infectious Clone ◽  
Mosquito Population ◽  
Mosquito Vector ◽  
Metagenomic Sequencing ◽  
Virus Serotype

Dengue virus is the most prevalent mosquito-borne virus, causing approximately 390 million infections and 25,000 deaths per year. Aedes aegypti, the primary mosquito vector of dengue virus, is well-established throughout the state of Florida, United States. Autochthonous transmission of dengue virus to humans in Florida has been increasing since 2009, alongside consistent importation of dengue cases. However, most cases of first infection with dengue are asymptomatic and the virus can be maintained in mosquito populations, complicating surveillance and leading to an underestimation of disease risk. Metagenomic sequencing of A. aegypti mosquitoes in Manatee County, Florida revealed the presence of dengue virus serotype 4 (DENV-4) genomes in mosquitoes from multiple trapping sites over 2years, in the absence of a human DENV-4 index case, and even though a locally acquired case of DENV-4 has never been reported in Florida. This finding suggested that: (i) DENV-4 may circulate among humans undetected; (ii) the virus was being maintained in the mosquito population, or (iii) the detected complete genome sequence may not represent a viable virus. This study demonstrates that an infectious clone generated from the Manatee County DENV-4 (DENV-4M) sequence is capable of infecting mammalian and insect tissue culture systems, as well as adult female A. aegypti mosquitoes when fed in a blood meal. However, the virus is subject to a dose dependent infection barrier in mosquitoes, and has a kinetic delay compared to a phylogenetically related wild-type (WT) control virus from a symptomatic child, DENV-4H (strain Homo sapiens/Haiti-0075/2015, GenBank accession MK514144.1). DENV-4M disseminates from the midgut to the ovary and saliva at 14days post-infection. Viral RNA was also detectable in the adult female offspring of DENV-4M infected mosquitoes. These results demonstrate that the virus is capable of infecting vector mosquitoes, is transmissible by bite, and is vertically transmitted, indicating a mechanism for maintenance in the environment without human-mosquito transmission. These findings suggest undetected human-mosquito transmission and/or long-term maintenance of the virus in the mosquito population is occurring in Florida, and underscore the importance of proactive surveillance for viruses in mosquitoes.GRAPHICAL ABSTRACTIn order to better assess the public health risk posed by a detection of DENV-4 RNA in Manatee County, FL Aedes aegypti, we produced an infectious clone using the sequence from the wild-caught mosquitoes and characterized it via laboratory infections of mosquitoes and mosquito tissues.

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