Family level variation in Wolbachia-mediated dengue virus blocking in Aedes aegypti

AbstractCompetition between viruses and Wolbachia for host lipids is a proposed mechanism of Wolbachia-mediated virus blocking in insects. Yet, the metabolomic interaction between virus and symbiont within the mosquito has not been clearly defined. We compare the lipid profiles of Aedes aegypti mosquitoes bearing mono- or dual-infections of the Wolbachia wMel strain and dengue virus serotype 3 (DENV3). We found metabolic signatures of infection-induced intracellular events but little evidence to support direct competition between Wolbachia and virus for host lipids. Lipid profiles of dual-infected mosquitoes resemble those of DENV3 mono-infected mosquitoes, suggesting virus-driven modulation dominates over that of Wolbachia. Interestingly, knockdown of key metabolic enzymes suggests cardiolipins are host factors for DENV3 and Wolbachia replication. These findings define the Wolbachia-DENV3 metabolic interaction as indirectly antagonistic, rather than directly competitive, and reveal new research avenues with respect to mosquito × virus interactions at the molecular level.

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Detection of dengue virus in Aedes mosquitoes in Delhi, India

ENTOMON ◽

10.33307/entomon.v44i3.466 ◽

2019 ◽

Vol 44 (3) ◽

pp. 213-218

Author(s):

Suresh Chand Kaushik ◽

Sukhvir Singh ◽

Purnima Srivastava ◽

R. Rajendran

Keyword(s):

Early Intervention ◽

Early Detection ◽

Aedes Aegypti ◽

Dengue Virus ◽

Infection Rate ◽

Transmission Season ◽

Aedes Mosquitoes ◽

Alternative Approach ◽

Human Sera ◽

Minimum Infection Rate

Detection of viruses in human sera particularly in endemic areas is cumbersome and laborious. Therefore, an alternative approach, Immuno-fluorescence assay (IFA) was performed to determine dengue virus (DENV) positivity in mosquitoes. A total of 1055 adult Aedes aegypti female mosquitoes were tested for IFA test against DENV. Minimum infection rate (MIR) for DENV was found higher during August to November 2016 ranging from 10.75 to 20.83. The average yearly MIR was about 6.64. Higher MIR for Ae. aegypti was found in Sarfabad, Noida (12.71) and Khoda Colony, Ghaziabad (11.90). Minimum MIR (4.67) was observed in Sanjay colony (Faridabad). The main contribution of this study resides in the development of a more suitable monitoring system for early detection of viral circulation and to prioritize early intervention in the non-transmission season.

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Difusi dan Pola Spasial Sebaran Penyakit Demam Berdarah Dengue (DBD) Di Kota Bandar Lampung

KESMARS Jurnal Kesehatan Masyarakat Manajemen dan Administrasi Rumah Sakit ◽

10.31539/kesmars.v1i1.192 ◽

2018 ◽

Vol 1 (1) ◽

pp. 87-95

Author(s):

Nurul Qamila ◽

Agel Vidian Krama

Keyword(s):

Public Health ◽

Spatial Analysis ◽

Aedes Aegypti ◽

Dengue Virus ◽

Spatial Pattern ◽

Dengue Hemorrhagic Fever ◽

Health Problem ◽

Public Health Problem ◽

Hemorrhagic Fever ◽

Descriptive Method

Dengue hemorrhagic fever (DHF) is a contagious disease caused by the dengue virus and is transmitted by the mosquito Aedes aegypti (Aa.aegypti). The population is still a public health problem that increases the number of sufferers and also widespread, with population and education. This study aims to reveal the spatial pattern and distribution of Dengue Hemorrhagic Fever (DHF) with the spatial pattern and the spread of Dengue Hemorrhagic Fever (DHF) can result in different locations of these allegations. From the map that can be used for the prevention of Dengue Hemorrhagic Fever (DBD) in Bandar Lampung City. This study aims to reveal the spatial pattern and distribution of Dengue Hemorrhagic Fever (DHF) with the descriptive method and spatial pattern of Dengue Hemorrhagic Fever (DHF) can result in different locations of these allegations. From the map that can be used for the prevention of Dengue Hemorrhagic Fever (DBD) in Bandar Lampung City. Keywords: DHF, Spatial Analysis

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Faculty Opinions recommendation of Engineering RNA interference-based resistance to dengue virus type 2 in genetically modified Aedes aegypti.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1030708.367881 ◽

2006 ◽

Author(s):

Vishvanath Nene

Keyword(s):

Rna Interference ◽

Aedes Aegypti ◽

Dengue Virus ◽

Virus Type ◽

Genetically Modified ◽

Dengue Virus Type 2

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Faculty Opinions recommendation of Field- and clinically derived estimates of Wolbachia-mediated blocking of dengue virus transmission potential in Aedes aegypti mosquitoes.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.732369982.793541519 ◽

2018 ◽

Cited By ~ 2

Author(s):

Jean-Luc Imler ◽

Nelson Martins

Keyword(s):

Aedes Aegypti ◽

Dengue Virus ◽

Virus Transmission ◽

Transmission Potential

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The Lethal(2)-Essential-for-Life [L(2)EFL] Gene Family Modulates Dengue Virus Infection in Aedes aegypti

International Journal of Molecular Sciences ◽

10.3390/ijms21207520 ◽

2020 ◽

Vol 21 (20) ◽

pp. 7520

Author(s):

Lucky R. Runtuwene ◽

Shuichi Kawashima ◽

Victor D. Pijoh ◽

Josef S. B. Tuda ◽

Kyoko Hayashida ◽

...

Keyword(s):

Aedes Aegypti ◽

Dengue Virus ◽

Cell Line ◽

Dengue Infection ◽

Fruit Fly ◽

Initiation Factor ◽

Poly I:C ◽

Gene Products ◽

Vector Mosquito

Efforts to determine the mosquito genes that affect dengue virus replication have identified a number of candidates that positively or negatively modify amplification in the invertebrate host. We used deep sequencing to compare the differential transcript abundances in Aedes aegypti 14 days post dengue infection to those of uninfected A. aegypti. The gene lethal(2)-essential-for-life [l(2)efl], which encodes a member of the heat shock 20 protein (HSP20) family, was upregulated following dengue virus type 2 (DENV-2) infection in vivo. The transcripts of this gene did not exhibit differential accumulation in mosquitoes exposed to insecticides or pollutants. The induction and overexpression of l(2)efl gene products using poly(I:C) resulted in decreased DENV-2 replication in the cell line. In contrast, the RNAi-mediated suppression of l(2)efl gene products resulted in enhanced DENV-2 replication, but this enhancement occurred only if multiple l(2)efl genes were suppressed. l(2)efl homologs induce the phosphorylation of eukaryotic initiation factor 2α (eIF2α) in the fruit fly Drosophila melanogaster, and we confirmed this finding in the cell line. However, the mechanism by which l(2)efl phosphorylates eIF2α remains unclear. We conclude that l(2)efl encodes a potential anti-dengue protein in the vector mosquito.

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Use of immunodot blot and multiplex reverse transcriptase-polymerase chain reaction in dengue virus detection in macerates of Aedes aegypti larvae

Microbiology Research ◽

10.4081/mr.2012.e13 ◽

2012 ◽

Vol 3 (1) ◽

pp. 13

Author(s):

Aline T.A. Chagas ◽

Michelle D. Oliveira ◽

Jose M.S. Mezencio ◽

Eduardo A.M. Silva ◽

Leandro L. Oliveira ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Aedes Aegypti ◽

Dengue Virus ◽

Reverse Transcriptase ◽

Epidemiological Surveillance ◽

Rt Pcr ◽

Chain Reaction ◽

Denv Serotypes ◽

Polymerase Chain ◽

Pcr Techniques

The <em>Dengue virus</em> is the main arbovirus that affects man in terms of morbidity and mortality. The detection of the virus is very important for epidemiological surveillance, so here we propose to standardize and compare the immunodot blot (IDB) and multiplex reverse transcriptase-polymerase chain reaction (M-RT-PCR) techniques to detect and characterize the dengue virus (DENV) serotypes in samples of <em>Aedes aegypti</em> larvae. Thus, the IDB and M-RT-PCR techniques were standardized using macerated samples of larvae collected in nature. The use of monoclonal antibodies in IDB has not shown great results, but DENV detection through this method was possible using polyclonal antibodies. The distinction of serotypes 1, 2 and 3 was carried out by M-RT-PCR.

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