scholarly journals Determination of free L-carnitine levels in type II diabetic women with and without complications

2007 ◽  
Vol 61 (7) ◽  
pp. 892-895 ◽  
Author(s):  
A Poorabbas ◽  
F Fallah ◽  
J Bagdadchi ◽  
R Mahdavi ◽  
A Aliasgarzadeh ◽  
...  
Keyword(s):  
Type Ii

Human Umbilical Cord Mesenchymal Stem Cells Induce into Chondrocytes in 3D Cultured System

2013 ◽  
Vol 749 ◽  
pp. 198-205
Author(s):  
Li Yu ◽  
Jing Liu ◽  
Chao Xu ◽  
Er Mei Luo ◽  
Ming Qiao Tang
Keyword(s):  
Real Time ◽  
Alcian Blue ◽  
Culture System ◽  
Type Ii Collagen ◽  
Human Umbilical Cord ◽  
Type Ii ◽  
Pellet Culture ◽  
Hla Dr

Objective: To investigate a better method of inducing hUC-MSCs into chondrocytes in different culture system in vitro. Method: hUC-MSCs were isolated and cultured by tissue block culture, and the cells surface antigens were identified by flow cytometry, hUC-MSCs were cultured with chondrogenic media and stained with Alcian Blue. The production of matrix was estimated from the determination of hydroxyproline content and Alcian Blue method. Expressions of glycosaminoglycan (GAG), type II collagen and Sox-9 were assayed by real-time fluorescence quantitative PCR. Results: The cultured hUC-MSCs phenotype was CD105+/CD29+/CD44+/ CD31-/CD34-/ CD40-/CD45-/HLA-DR-. hUC-MSCs weakly expressed chondrocyte marker, which strongly expressed GAG and type II collagen after chondrogenic induction, and the cells were incubated in pellet culture with higher expression. Real-time PCR results demonstrated that chondrogenic induction cells were expressed GAG, type II collagen and Sox-9, and the cells were incubated in pellet culture with higher expression. Conclusion: hUC-MSCs incubated in pellet culture is more conducive to differentiate into chondrocytes than those cultured in monolayer culture system.

Administration of a Mixture of Fungal Glucosidases to a Patient with Type II Glycogenosis (Pompe's Disease)

PEDIATRICS ◽  
1968 ◽  
Vol 42 (4) ◽  
pp. 672-676
Author(s):  
Ronald M. Lauer ◽  
Thelma Mascarinas ◽  
Antonio S. Racela ◽  
Antoni M. Diehl ◽  
Barbara Illingworth Brown
Keyword(s):  
Skeletal Muscle ◽  
Enzyme Assay ◽  
Glycogen Content ◽  
Hydrolytic Enzymes ◽  
Type Ii ◽  
Electron Microscopic ◽  
Pompe’S Disease ◽  
Pompe's Disease ◽  
Type Ii Glycogenosis

A case of Type II glycogenosis (Pompe's disease) has been studied by histochemical, electron microscopic, and biochemical techniques. These studies have been made prior to and after the intramuscular administration for 1 week of a mixture of hydrolytic enzymes containing both α-1,4- and α-1,6-glucosidase activities. Electron photomicrographs of the liver before enzyme administration showed glycogen to be located both within and outside of membrane-limited vacuoles. No change in this distribution could be detected in tissue removed by biopsy after enzyme administration. This impression was confirmed by the determination of glycogen content which was shown to be unchanged. Nevertheless, the liver was found by enzyme assay to contain the administered enzyme. Leucocytes isolated from blood taken 4 hours after the last enzyme injection were also shown to contain the parenterally administered glucosidases. In skeletal muscle glycogen was present chiefly as extrasaccular deposits which were unchanged in appearance by enzyme administration. No glucosidase activity was demonstrable in the skeletal muscle after such a treatment. Myocardium sectioned after autopsy had major deposits of glycogen in extrasaccular areas.

scholarly journals DETERMINATION OF CROSS SECTIONS FOR GRAVITY TYPE QUAY WALLS

2017 ◽  
pp. 5
Author(s):  
Tugce Yuksel ◽  
Yalcin Yuksel ◽  
Busra Basaran ◽  
Esin Cevik
Keyword(s):  
Cross Sections ◽  
Vertical Wall ◽  
Block Size ◽  
Seismic Loading ◽  
Block Type ◽  
Type I ◽  
Type Ii ◽  
Seismic Loadings ◽  
Port Structures

Block type quay walls are widely used as port structures in the world. In this study three types of vertical block type quay walls with different block size exposed to seismic loading were investigated experimentally. The block ratios of Type I, Type II and III vertical wall models are B/h=2, 1.5 and 1.5 & H/h=6, 6 and 3, respectively. The tests were conducted in the shaking tank with different harmonic seismic loadings and the behaviors of these walls were investigated comparatively.

The Determination of Macrostresses (Type-I Stresses) and Microstresses (Type-Ii Stresses) by Neutron Diffraction

1994 ◽  
Vol 376 ◽  
Author(s):  
T.M. Holden
Keyword(s):  
Neutron Diffraction ◽  
Experimental Methods ◽  
Stress Fields ◽  
Type I ◽  
Type Ii ◽  
Sources Of Error ◽  
Precision And Accuracy

ABSTRACTNeutron diffraction has been used as tool for measuring stress fields (type-I stresses) and intergranular or microstresses (type-II stresses) for a decade. The experimental methods for measuring the two types of stresses are outlined. Emphasis is placed on maximizing the precision and accuracy of the measurements. Some serious sources of error in measurements near surfaces and some subtle wavelength effects are considered.

Baade-Wesselink Radius Determination of Type II Cepheids

1997 ◽  
Vol 113 ◽  
pp. 1833 ◽  
Author(s):  
Z. Balog ◽  
J. Vinko ◽  
G. Kaszas
Keyword(s):  
Type Ii

scholarly journals Type II Supernova Photospheres and the Distance to Supernova 1987A

1988 ◽  
Vol 108 ◽  
pp. 295-304
Author(s):  
Manorama Chilukuri ◽  
Robert V. Wagoner
Keyword(s):  
Time Scales ◽  
Direct Determination ◽  
Type Ii ◽  
Physical Conditions ◽  
Supernova 1987A ◽  
Spectral Flux ◽  
Spectrophotometric Data ◽  
Parallel Geometry ◽  
The Many

Among the many historic opportunities provided by the recent supernova in the LMC is that to improve our understanding of the physical conditions in the neighborhood of supernova photospheres, even though 1987A was initially characterized by radial and time scales smaller (by a factor 5–10) than “standard” more luminous SNII. Two consequences of this understanding, which we shall focus on in this contribution, are (a) an estimate of the (frequency-dependent) location and thickness of the photosphere and (b) the only direct determination of the distance of the supernova (via the generalized Baade method). We find that the photosphere is sharp enough to allow the use of plane-parallel geometry in the calculation of the emergent continuum spectral flux, if we confine our attention to those epochs (temperature T ~ 5000−6000 K) at which hydrogen is recombining at the photosphere. We also find that the distance to this supernova is 43 ± 4 kpc. The reliability of this determination should improve when accurate spectrophotometric data for dates other than March 1 become available to us.

Quantitation of Beta Lipoprotein (LDL) Cholesterol by Densitometric Evaluation of Disc Electropherograms

1972 ◽  
Vol 18 (3) ◽  
pp. 217-221 ◽  
Author(s):  
Robert F Moran ◽  
William P Castelli ◽  
Marianne V Moran
Keyword(s):  
Ldl Cholesterol ◽  
Electrophoretic Pattern ◽  
Disc Electrophoresis ◽  
Polyacrylamide Gel ◽  
Type Ii ◽  
Chemical Concentration ◽  
Type Iv ◽  
Chemical Technique ◽  
Peak Areas

Abstract This paper describes a rapid, facile, and accurate new procedure for determination of marginal Type IV or Type II hyperlipoproteinemias, in which discontinuous (disc) electrophoresis on polyacrylamide gel is used to quantitate LDL (beta) cholesterol without ultracentrifugation. Fasting sera, previously assayed by ultracentrifugation and chemical technique, were prestained with Sudan Black, and the lipoproteins were separated by disc electrophoresis. The beta-lipoprotein fraction of each electrophoretic pattern was quantitated by densitometry. The values for the integrated densitometric peak areas for the beta fraction and the chemically assayed cholesterol of the ultracentrifuged LDL fraction correlated well (r = .90). Results were determined from a curve relating chemical concentration of the integrated peak values by densitometry to the known LDL cholesterol of a series of standards.

The Development of Two Fluorimetric Assays for the Determination of Pyroglutamyl Aminopeptidase Type-II Activity

1997 ◽  
Vol 250 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Seán P. Gallagher ◽  
Rhona M. O'Leary ◽  
Brendan O'Connor
Keyword(s):  
Type Ii
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