scholarly journals Dual promoters of the major catalase (KatA) govern distinct survival strategies of Pseudomonas aeruginosa

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
In-Young Chung ◽  
Bi-o Kim ◽  
Hye-Jeong Jang ◽  
You-Hee Cho
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Survival Strategies ◽  
Dual Promoters

scholarly journals SURVIVAL STRATEGIES OF PSEUDOMONAS AERUGINOSA IN THE RESPIRATORY TRACT IN CYSTIC FIBROSIS

2021 ◽  
Vol 2 (1) ◽  
pp. 29
Author(s):  
O. V. Ishchenko ◽  
A. О. Efimenko ◽  
O. Andriiashyna ◽  
I. P. Koshova ◽  
D. O. Stepanskyi
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Respiratory Tract ◽  
Survival Strategies

scholarly journals Computationally designed pyocyanin demethylase acts synergistically with tobramycin to kill recalcitrant Pseudomonas aeruginosa biofilms

2021 ◽  
Vol 118 (12) ◽  
pp. e2022012118
Author(s):  
Chelsey M. VanDrisse ◽  
Rosalie Lipsh-Sokolik ◽  
Olga Khersonsky ◽  
Sarel J. Fleishman ◽  
Dianne K. Newman
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Protein Design ◽  
Chronic Wounds ◽  
Fold Increase ◽  
Survival Strategies ◽  
Design Algorithm ◽  
Hypoxic Conditions ◽  
Opportunistic Human Pathogen ◽  
Biofilm Development

Pseudomonas aeruginosa is an opportunistic human pathogen that develops difficult-to-treat biofilms in immunocompromised individuals, cystic fibrosis patients, and in chronic wounds. P. aeruginosa has an arsenal of physiological attributes that enable it to evade standard antibiotic treatments, particularly in the context of biofilms where it grows slowly and becomes tolerant to many drugs. One of its survival strategies involves the production of the redox-active phenazine, pyocyanin, which promotes biofilm development. We previously identified an enzyme, PodA, that demethylated pyocyanin and disrupted P. aeruginosa biofilm development in vitro. Here, we asked if this protein could be used as a potential therapeutic for P. aeruginosa infections together with tobramycin, an antibiotic typically used in the clinic. A major roadblock to answering this question was the poor yield and stability of wild-type PodA purified from standard Escherichia coli overexpression systems. We hypothesized that the insufficient yields were due to poor packing within PodA’s obligatory homotrimeric interfaces. We therefore applied the protein design algorithm, AffiLib, to optimize the symmetric core of this interface, resulting in a design that incorporated five mutations leading to a 20-fold increase in protein yield from heterologous expression and purification and a substantial increase in stability to environmental conditions. The addition of the designed PodA with tobramycin led to increased killing of P. aeruginosa cultures under oxic and hypoxic conditions in both the planktonic and biofilm states. This study highlights the potential for targeting extracellular metabolites to assist the control of P. aeruginosa biofilms that tolerate conventional antibiotic treatment.

Multiple erythematous nodules as a manifestation of Pseudomonas aeruginosa septicemia

1980 ◽  
Vol 116 (4) ◽  
pp. 446-447 ◽  
Author(s):  
D. Schlossberg
Keyword(s):  
Pseudomonas Aeruginosa

306: Relationships between Biofilm-Forming Capacities of Pseudomonas Aeruginosa Isolates and Clinical Background / Antimicrobial Resistence in Urinary Tract Infections

2007 ◽  
Vol 177 (4S) ◽  
pp. 102-103
Author(s):  
Shinya Uehara ◽  
Koichi Monden ◽  
Koichiro Wada ◽  
Ayano Ishii ◽  
Reiko Kariyama ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Clinical Background ◽  
Tract Infections

Analytic Survival Strategies

1996 ◽  
Author(s):  
Maureen Murphy ◽  
Karen Rosica
Keyword(s):  
Survival Strategies

Green striped nails: chromonychia due to Pseudomonas aeruginosa

1968 ◽  
Vol 97 (2) ◽  
pp. 149-153 ◽  
Author(s):  
W. V. Shellow
Keyword(s):  
Pseudomonas Aeruginosa

Effekt von Acetylcystein auf die Pseudomonas aeruginosa-induzierte Mukusexpression

Pneumologie ◽  
2010 ◽  
Vol 64 (01) ◽  
Author(s):  
L Sprenger ◽  
T Goldmann ◽  
E Vollmer ◽  
B Wollenberg ◽  
P Zabel ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa

Effekt von Dexamethason auf die Pseudomonas aeruginosa-induzierte Mukusexpression in humaner Atemwegsmukosa

Pneumologie ◽  
2010 ◽  
Vol 64 (S 03) ◽  
Author(s):  
L Spenger ◽  
T Goldmann ◽  
E Vollmer ◽  
B Wollenberg ◽  
HP Hauber ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa
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