Survival strategies of SMEs amidst the COVID-19 pandemic: application of SEM and fsQCA

Purpose As a global pandemic, the COVID-19 crisis has profoundly affected the development of local firms, threatening the survival of small and medium enterprises (SMEs). This study aims to present an integrated framework by investigating the impact of strategic tools (i.e. firms’ capability of business agility, marketing operational efficiency, optimisation of innovation capability [OIC], managing employees’ satisfaction and rethinking customers’ experience) on the survival strategies of SMEs amidst the COVID-19 pandemic. Design/methodology/approach The current study used data from managers of SMEs and conducted an asymmetrical analysis (i.e. structural equation modelling [SEM]) to investigate the factors influencing the survival strategies of SMEs amidst the COVID-19 pandemic. This study also applied an asymmetrical approach (i.e. fuzzy sets qualitative comparative analysis-fsQCA) to explore the causal recipes and analysis of the necessary conditions to identify the factors required to achieve the expected outcome. Findings Results from SEM support all hypotheses. Results from fsQCA with the same data set show that firms’ business agility and OIC are necessary conditions for SMEs’ survival strategies. The result from fsQCA also reveals multiple sufficient conditions to succeed SMEs’ survival strategies amidst the COVID-19 pandemic. Practical implications Findings prescribe how SMEs adapt to this vulnerable business condition by applying the strategic tools and recipes suggested for survival. Originality/value This research applied an innovative analysis to reveal necessary and sufficient conditions that conventional methods such as SEM have limited power. This pioneering research in the context of the COVID-19 pandemic is considered novel in terms of the prescriptive strategic recipes offered to SMEs to adapt to and survive in the crisis caused by COVID-19.

Metabolic switching and cell wall remodelling of Mycobacterium tuberculosis during bone tuberculosis

Bone tuberculosis is widely characterized by irreversible bone destruction caused by Mycobacterium tuberculosis . Mycobacterium has the ability to adapt to various environmental stresses by altering its transcriptome in order to establish infection in the host. Thus, it is of critical importance to understand the transcriptional profile of M. tuberculosis during infection in the bone environment compared to axenic cultures of exponentially growing M.tb. In the current study, we characterized the in vivo transcriptome of M. tuberculosis within abscesses or necrotic specimens obtained from patients with bone TB using whole genome microarrays in order to gain insight into the M. tuberculosis adaptive response within this host microenvironment. A total of 914 mycobacterial genes were found to be significantly over-expressed and 1688 were repressed (fold change>2; p-value ≤ 0.05) in human bone TB specimens. Overall, the mycobacteria displayed a hypometabolic state with significant (p ≤ 0.05) downregulation of major pathways involved in translational machinery, cellular and protein metabolism and response to hypoxia. However, significant enrichment (p ≤ 0.05) of amino-sugar metabolic processes, membrane glycolipid biosynthesis, amino acid biosynthesis (serine, glycine, arginine and cysteine) and accumulation of mycolyl-arabinogalactan-peptidoglycan complex suggests possible mycobacterial survival strategies within the bone lesions by strengthening its cell wall and cellular integrity. Data were also screened for M.tb virulence proteins using Virulent-Pred and VICM-Pred tools, which revealed five genes (Rv1046c, Rv1230c, DppD, PE_PGRS26 and PE_PGRS43) with a possible role in the pathogenesis of bone TB. Next, an osteoblast cell line model for bone TB was developed allowing for significant intracellular multiplication of M.tb. Interestingly, three virulence genes (Rv1046c, DppD and PE_PGRS26) identified from human bone TB microarray data were also found to be overexpressed by intracellular M. tuberculosis in osteoblast cell lines. Overall, these data demonstrate that M. tuberculosis alters its transcriptome as an adaptive strategy to survive in the host and establish infection in bone. Additionally, the in vitro osteoblast model we describe may facilitate our understanding of the pathogenesis of bone TB.

“‘It ain’t have no sort of family life for us here’”: Community and its Discontents in Sam Selvon’s The Lonely Londoners

<div>Sam Seldon's The Lonely Londoners depicts an emergent collectivity of black immigrants who lead literally and metaphorically subterranean lives in 1950s Britain. Against the backdrop of a city undergoing an ambivalent transition from colonial metropole to postmodern cosmopolis, Seldon's "boys" remain largely inscrutable to and estranged from not only white Londoners but also one another. Critics have associate a depoliticized preoccupation with the everyday and eschewal of critical consciousness in Seldon’s work with widely critiqued features of Anglophone modernism. The present analysis suggests several reasons why political collectivity remains elusive to Seldon's black male immigrant characters. Specifically, they face discriminatory access to the labor market and social services, loci of possible solidarity with working-class white Londoners where formal political resistance might be coordinated. These systemic pressures combined with an atmospheric racism cause many of the boys to internalize the racialist, individualist, consumerist, and heterosexist attitudes and behaviors of the dominant white culture, which they adopt as survival strategies, in effect undermining black group identity and cohesion. If a note of optimism is to be sounded amid the many challenges to inter- and intraracial community the novel presents, it is in the potential undoing of black cultural nationalism that cultural theorist Paul Gilroy sees as a crucial step in the making of an egalitarian, convivial postcolonial world. The novel contests the homogenizing impulses of essentialist identity politics by portraying the heterodox, sometimes paradoxical, affinities that emerge between characters and communities.</div>

“‘It ain’t have no sort of family life for us here’”: Community and its Discontents in Sam Selvon’s The Lonely Londoners

<div>Sam Seldon's The Lonely Londoners depicts an emergent collectivity of black immigrants who lead literally and metaphorically subterranean lives in 1950s Britain. Against the backdrop of a city undergoing an ambivalent transition from colonial metropole to postmodern cosmopolis, Seldon's "boys" remain largely inscrutable to and estranged from not only white Londoners but also one another. Critics have associate a depoliticized preoccupation with the everyday and eschewal of critical consciousness in Seldon’s work with widely critiqued features of Anglophone modernism. The present analysis suggests several reasons why political collectivity remains elusive to Seldon's black male immigrant characters. Specifically, they face discriminatory access to the labor market and social services, loci of possible solidarity with working-class white Londoners where formal political resistance might be coordinated. These systemic pressures combined with an atmospheric racism cause many of the boys to internalize the racialist, individualist, consumerist, and heterosexist attitudes and behaviors of the dominant white culture, which they adopt as survival strategies, in effect undermining black group identity and cohesion. If a note of optimism is to be sounded amid the many challenges to inter- and intraracial community the novel presents, it is in the potential undoing of black cultural nationalism that cultural theorist Paul Gilroy sees as a crucial step in the making of an egalitarian, convivial postcolonial world. The novel contests the homogenizing impulses of essentialist identity politics by portraying the heterodox, sometimes paradoxical, affinities that emerge between characters and communities.</div>

Regulation of major bacterial survival strategies by transcript sequestration in a membraneless organelle

Liquid-liquid phase separation (LLPS) of proteins was shown in recent years to regulate spatial organization of cell content without the need for membrane encapsulation in eukaryotes and prokaryotes. Yet evidence for the relevance of LLPS for bacterial cell functionality is largely missing. Here we show that the sugar metabolism-regulating clusters, recently shown by us to assemble in the E. coli cell poles by means of the novel protein TmaR, are formed via LLPS. A mutant screen uncovered residues and motifs in TmaR that are important for its condensation. Upon overexpression, TmaR undergoes irreversible liquid-to-solid transition, similar to the transition of disease-causing proteins in human, which impairs bacterial cell morphology and proliferation. Not only does RNA contribute to TmaR phase separation, but by ensuring polar localization and stability of flagella-related transcripts, TmaR enables cell motility and biofilm formation, thus providing a linkage between LLPS and major survival strategies in bacteria.

The CovRS Environmental Sensor Directly Controls the ComRS Signaling System To Orchestrate Competence Bimodality in Salivarius Streptococci

Combining production of antibacterial compounds and uptake of DNA material released by dead cells, competence is one of the most efficient survival strategies in streptococci. Yet, this powerful tactic is energy consuming and reprograms the metabolism to such an extent that cell proliferation is transiently impaired.

Robust Heat Shock Response in Chlamydia Lacking a Typical Heat Shock Sigma Factor

Cells reprogram their transcriptome in response to stress, such as heat shock. In free-living bacteria, the transcriptomic reprogramming is mediated by increased DNA-binding activity of heat shock sigma factors and activation of genes normally repressed by heat-induced transcription factors. In this study, we performed transcriptomic analyses to investigate heat shock response in the obligate intracellular bacterium Chlamydia trachomatis, whose genome encodes only three sigma factors and a single heat-induced transcription factor. Nearly one-third of C. trachomatis genes showed statistically significant (≥1.5-fold) expression changes 30 min after shifting from 37 to 45°C. Notably, chromosomal genes encoding chaperones, energy metabolism enzymes, type III secretion proteins, as well as most plasmid-encoded genes, were differentially upregulated. In contrast, genes with functions in protein synthesis were disproportionately downregulated. These findings suggest that facilitating protein folding, increasing energy production, manipulating host activities, upregulating plasmid-encoded gene expression, and decreasing general protein synthesis helps facilitate C. trachomatis survival under stress. In addition to relieving negative regulation by the heat-inducible transcriptional repressor HrcA, heat shock upregulated the chlamydial primary sigma factor σ66 and an alternative sigma factor σ28. Interestingly, we show for the first time that heat shock downregulates the other alternative sigma factor σ54 in a bacterium. Downregulation of σ54 was accompanied by increased expression of the σ54 RNA polymerase activator AtoC, thus suggesting a unique regulatory mechanism for reestablishing normal expression of select σ54 target genes. Taken together, our findings reveal that C. trachomatis utilizes multiple novel survival strategies to cope with environmental stress and even to replicate. Future strategies that can specifically target and disrupt Chlamydia’s heat shock response will likely be of therapeutic value.

Adaptive Leadership in Micro Social Enterprise Teams

COVID-19 is a relatively new disease, and most victims are in low income, people-facing jobs. The most economically disadvantaged in society are less able to comply with certain non-pharmaceutical interventions (NPIs) partly due to their financial situation. Hope for the Helpless (H4H), set up as part of a micro social enterprise to cater for rough sleepers in London, UK, is at the heart of the COVID-19 response to the homeless there. A lack of financial resources or capital can constrain social entrepreneurs and restrict their ability to create social capital. However, some social entrepreneurs have been able to innovatively leverage on previous relationships to augment their resource base because engagement with stakeholders is critical. The study explores one such relationship between H4H and a local health centre. It is hoped that this study will uncover survival strategies for social entrepreneurs to succeed and thrive in a post-COVID-19 world.