scholarly journals Population genomics of C. melanopterus using target gene capture data: demographic inferences and conservation perspectives

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Pierpaolo Maisano Delser ◽  
Shannon Corrigan ◽  
Matthew Hale ◽  
Chenhong Li ◽  
Michel Veuille ◽  
...  
Keyword(s):  
Target Gene ◽  
Population Genomics ◽  
Gene Capture ◽  
Target Gene Capture

scholarly journals Population genomics ofC. melanopterususing target gene capture data: demographic inferences and conservation perspectives

2016 ◽  
Author(s):  
Pierpaolo Maisano Delser ◽  
Shannon Corrigan ◽  
Matthew Hale ◽  
Chenhong Li ◽  
Michel Veuille ◽  
...  
Keyword(s):  
Target Gene ◽  
Population Genomics ◽  
Model Organism ◽  
Sampling Scheme ◽  
Model Organisms ◽  
Gene Capture ◽  
Sampling Schemes ◽  
History Of ◽  
Carcharhinus Melanopterus ◽  
Target Gene Capture

AbstractPopulation genetics studies on non-model organisms typically involve sampling few markers from multiple individuals. Next-generation sequencing approaches open up the possibility of sampling many more markers from fewer individuals to address the same questions. Here, we applied a target gene capture method to deep sequence ∼1000 independent autosomal regions of a non-model organism, the blacktip reef shark (Carcharhinus melanopterus). We devised a sampling scheme based on the predictions of theoretical studies of metapopulations to show that sampling few individuals, but many loci, can be extremely informative to reconstruct the evolutionary history of species. We collected data from a single deme (SID) from Northern Australia and from a scattered sampling representing various locations throughout the Indian Ocean (SCD). We explored the genealogical signature of population dynamics detected from both sampling schemes using an ABC algorithm. We then contrasted these results with those obtained by fitting the data to a non-equilibrium finite island model. Both approaches supported anNmvalue ∼40, consistent with philopatry in this species. Finally, we demonstrate through simulation that metapopulations exhibit greater resilience to recent changes in effective size compared to unstructured populations. We propose an empirical approach to detect recent bottlenecks based on our sampling scheme.

Molecular Diagnostic Approaches to Metabolic Myopathies by Target Gene Capture and Next Generation Sequencing (P07.215)

Neurology ◽  
2012 ◽  
Vol 78 (Meeting Abstracts 1) ◽  
pp. P07.215-P07.215
Author(s):  
V. Zhang ◽  
H. Cui ◽  
J. Wang ◽  
L.-j. Wong
Keyword(s):  
Next Generation Sequencing ◽  
Target Gene ◽  
Molecular Diagnostic ◽  
Next Generation ◽  
Gene Capture ◽  
Metabolic Myopathies ◽  
Diagnostic Approaches ◽  
Generation Sequencing ◽  
Target Gene Capture

scholarly journals Improved molecular diagnosis by the detection of exonic deletions with target gene capture and deep sequencing

2014 ◽  
Vol 17 (2) ◽  
pp. 99-107 ◽  
Author(s):  
Yanming Feng ◽  
David Chen ◽  
Guo-Li Wang ◽  
Victor Wei Zhang ◽  
Lee-Jun C. Wong
Keyword(s):  
Molecular Diagnosis ◽  
Deep Sequencing ◽  
Target Gene ◽  
Gene Capture ◽  
Target Gene Capture

scholarly journals Target Gene Capture Sequencing in Chinese Population of Sporadic Parkinson Disease

Medicine ◽  
2015 ◽  
Vol 94 (20) ◽  
pp. e836 ◽  
Author(s):  
Zhiming Li ◽  
Qing Lin ◽  
Wenqing Huang ◽  
Chi-Meng Tzeng
Keyword(s):  
Parkinson Disease ◽  
Chinese Population ◽  
Target Gene ◽  
Gene Capture ◽  
Capture Sequencing ◽  
Target Gene Capture

scholarly journals Identification of a Novel ACTN4 Gene Mutation Which Is Resistant to Primary Nephrotic Syndrome Therapy

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Lingzhang Meng ◽  
Shan Cao ◽  
Na Lin ◽  
Jingjie Zhao ◽  
Xulong Cai ◽  
...  
Keyword(s):  
Nephrotic Syndrome ◽  
Target Gene ◽  
Minimal Change ◽  
Cleavage Sites ◽  
Primary Nephrotic Syndrome ◽  
Minimal Change Nephropathy ◽  
Filtration Barrier ◽  
Capture Method ◽  
Generation Sequencing ◽  
Target Gene Capture

ACTN4, a gene which codes for the protein α-actinin-4, is critical for the maintenance of the renal filtration barrier. It is well known that ACTN4 mutations can lead to kidney dysfunction, such as familial focal segmental glomerulosclerosis (FSGS), a common cause of primary nephrotic syndrome (PNS). To elucidate whether other mutations of ACTN4 exist in PNS patients, we sequenced the ACTN4 gene in biopsies collected from 155 young PNS patients (≤16 years old). The patients were classified into five groups: FSGS, minimal change nephropathy, IgA nephropathy, membranous nephropathy, and those without renal puncture. Ninety-eight healthy people served as controls. Samples were subjected to Illumina’s next generation sequencing protocols using FastTarget target gene capture method. We identified 5 ACTN4 mutations which occurred only in PNS patients: c.1516G > A (p.G506S) on exon 13 identified in two PNS patients, one with minimal change nephropathy and another without renal puncture; c.1442 + 10G > A at the splice site in a minimal change nephropathy patient; c.2191-4G > A at the cleavage site, identified from two FSGS patients; and c.1649A > G (p.D550G) on exon 14 together with c.2191-4G > A at the cleavage sites, identified from two FSGS patients. Among these, c.1649A > G (p.D550G) is a novel ACTN4 mutation. Patients bearing the last two mutations exhibited resistance to clinical therapies.

Muscle RAS oncogene homolog is a novel therapeutic target gene contributing to hepatocellular carcinoma progression and sorafenib-resistance

2018 ◽  
Vol 56 (01) ◽  
pp. E2-E89
Author(s):  
M Bildstein ◽  
C Hellerbrand ◽  
A Bosserhoff ◽  
P Dietrich
Keyword(s):  
Hepatocellular Carcinoma ◽  
Therapeutic Target ◽  
Target Gene ◽  
Ras Oncogene ◽  
Novel Therapeutic

Investigating miR-125b target gene Arid3a in trisomy 21-associated leukemogenesis

2020 ◽  
Author(s):  
K Weigert ◽  
O Alejo-Valle ◽  
M Labuhn ◽  
V Amstislavskiy ◽  
S Emmrich ◽  
...  
Keyword(s):  
Trisomy 21 ◽  
Target Gene

Identification of Id1 as target gene of profibrogenic TGF-β family signaling during transdifferentiation of hepatic stellate cells

2004 ◽  
Vol 42 (01) ◽  
Author(s):  
E Wiercinska ◽  
L Wickert ◽  
J Hamzavi ◽  
AM Gressner ◽  
S Dooley
Keyword(s):  
Hepatic Stellate Cells ◽  
Target Gene ◽  
Stellate Cells

Suppression of 11[beta]-hydroxysteroid dehydrogenase type 1 target gene regulation by hypoxia

2016 ◽  
Author(s):  
Bushra Shammout ◽  
Adewonuola Alase ◽  
Miriam Wittmann ◽  
Paul Stewart ◽  
Ana Tiganescu
Keyword(s):  
Gene Regulation ◽  
Target Gene ◽  
Hydroxysteroid Dehydrogenase
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