scholarly journals Increased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol metabolism in Pichia pastoris

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Elena Cámara ◽  
Nils Landes ◽  
Joan Albiol ◽  
Brigitte Gasser ◽  
Diethard Mattanovich ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Aox1 Promoter ◽  
Methanol Metabolism ◽  
Regulated Expression ◽  
Transcription Attenuation

Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast Pichia pastoris

1985 ◽  
Vol 5 (5) ◽  
pp. 1111-1121
Author(s):  
S B Ellis ◽  
P F Brust ◽  
P J Koutz ◽  
A F Waters ◽  
M M Harpold ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Alcohol Oxidase ◽  
Methylotrophic Yeasts ◽  
Sequence Analyses ◽  
Yeast Pichia Pastoris ◽  
Oxidation Of Methanol ◽  
Regulated Expression

The oxidation of methanol follows a well-defined pathway and is similar for several methylotrophic yeasts. The use of methanol as the sole carbon source for the growth of Pichia pastoris stimulates the expression of a family of genes. Three methanol-responsive genes have been isolated; cDNA copies have been made from mRNAs of these genes, and the protein products from in vitro translations have been examined. The identification of alcohol oxidase as one of the cloned, methanol-regulated genes has been made by enzymatic, immunological, and sequence analyses. Methanol-regulated expression of each of these three isolated genes can be demonstrated to occur at the level of transcription. Finally, DNA subfragments of two of the methanol-responsive genomic clones from P. pastoris have been isolated and tentatively identified as containing the control regions involved in methanol regulation.

Invertase secretion in Hansenula polymorpha under the AOX1 promoter from Pichia pastoris

Yeast ◽  
1996 ◽  
Vol 12 (9) ◽  
pp. 815-822 ◽  
Author(s):  
L. Rodriguez ◽  
R. E. Narciandi ◽  
H. Roca ◽  
J. Cremata ◽  
R. Montesinos ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Hansenula Polymorpha ◽  
Aox1 Promoter

scholarly journals Enhancing the efficiency of the Pichia pastoris AOX1 promoter via the synthetic positive feedback circuit of transcription factor Mxr1

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Ching-Hsiang Chang ◽  
Hao-An Hsiung ◽  
Kai-Lin Hong ◽  
Ching-Tsan Huang
Keyword(s):  
Transcription Factor ◽  
Pichia Pastoris ◽  
Positive Feedback ◽  
Feedback Circuit ◽  
Aox1 Promoter ◽  
Positive Feedback Circuit

Non-repressing carbon sources for alcohol oxidase (AOX1) promoter of Pichia pastoris

2001 ◽  
Vol 92 (6) ◽  
pp. 585-589 ◽  
Author(s):  
Mehmet Inan ◽  
Michael M. Meagher
Keyword(s):  
Pichia Pastoris ◽  
Carbon Sources ◽  
Alcohol Oxidase ◽  
Aox1 Promoter

Regulation of alcohol oxidase 1 (AOX1) promoter and peroxisome biogenesis in different fermentation processes in Pichia pastoris

2013 ◽  
Vol 166 (4) ◽  
pp. 174-181 ◽  
Author(s):  
Sehoon Kim ◽  
Shannon Warburton ◽  
Istvan Boldogh ◽  
Cecilia Svensson ◽  
Liza Pon ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Alcohol Oxidase ◽  
Peroxisome Biogenesis ◽  
Aox1 Promoter ◽  
Fermentation Processes

PpNrg1 is a transcriptional repressor for glucose and glycerol repression of AOX1 promoter in methylotrophic yeast Pichia pastoris

2015 ◽  
Vol 38 (2) ◽  
pp. 291-298 ◽  
Author(s):  
Xiaolong Wang ◽  
Menghao Cai ◽  
Lei Shi ◽  
Qi Wang ◽  
Jinxiang Zhu ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Transcriptional Repressor ◽  
Methylotrophic Yeast ◽  
Aox1 Promoter ◽  
Yeast Pichia Pastoris ◽  
Methylotrophic Yeast Pichia Pastoris

scholarly journals A Mobile PTS2 Receptor for Peroxisomal Protein Import in Pichia pastoris

1998 ◽  
Vol 140 (4) ◽  
pp. 807-820 ◽  
Author(s):  
Ype Elgersma ◽  
Minetta Elgersma-Hooisma ◽  
Thibaut Wenzel ◽  
J. Michael McCaffery ◽  
Marilyn G. Farquhar ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Protein Import ◽  
Subcellular Location ◽  
Chondrodysplasia Punctata ◽  
Rhizomelic Chondrodysplasia Punctata ◽  
Methanol Metabolism ◽  
Mutant Proteins ◽  
Peroxisomal Targeting ◽  
Signal Type ◽  
Model Protein

Abstract. Using a new screening procedure for the isolation of peroxisomal import mutants in Pichia pastoris, we have isolated a mutant (pex7) that is specifically disturbed in the peroxisomal import of proteins containing a peroxisomal targeting signal type II (PTS2). Like its Saccharomyces cerevisiae homologue, PpPex7p interacted with the PTS2 in the two-hybrid system, suggesting that Pex7p functions as a receptor. The pex7Δ mutant was not impaired for growth on methanol, indicating that there are no PTS2-containing enzymes involved in peroxisomal methanol metabolism. In contrast, pex7Δ cells failed to grow on oleate, but growth on oleate could be partially restored by expressing thiolase (a PTS2-containing enzyme) fused to the PTS1. Because the subcellular location and mechanism of action of this protein are controversial, we used various methods to demonstrate that Pex7p is both cytosolic and intraperoxisomal. This suggests that Pex7p functions as a mobile receptor, shuttling PTS2-containing proteins from the cytosol to the peroxisomes. In addition, we used PpPex7p as a model protein to understand the effect of the Pex7p mutations found in human patients with rhizomelic chondrodysplasia punctata. The corresponding PpPex7p mutant proteins were stably expressed in P. pastoris, but they failed to complement the pex7Δ mutant and were impaired in binding to the PTS2 sequence.

scholarly journals Correction: Enhancing monellin production by Pichia pastoris at low cell induction concentration via effectively regulating methanol metabolism patterns and energy utilization efficiency

PLoS ONE ◽  
2018 ◽  
Vol 13 (7) ◽  
pp. e0201085
Author(s):  
Luqiang Jia ◽  
Tingyong Tu ◽  
Qiangqiang Huai ◽  
Jiaowen Sun ◽  
Shanshan Chen ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Energy Utilization ◽  
Utilization Efficiency ◽  
Methanol Metabolism ◽  
Cell Induction
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