scholarly journals Resisting majesty: Apis cerana, has lower antennal sensitivity and decreased attraction to queen mandibular pheromone than Apis mellifera

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Shihao Dong ◽  
Ping Wen ◽  
Qi Zhang ◽  
Xinyu Li ◽  
Ken Tan ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Apis Cerana ◽  
Decanoic Acid ◽  
Enoic Acid ◽  
Queen Mandibular Pheromone ◽  
Social Bees ◽  
Specific Sensitivity ◽  
Evolutionarily Conserved ◽  
Ovarian Activation ◽  
Species Specific

Abstract In highly social bees, queen mandibular pheromone (QMP) is vital for colony life. Both Apis cerana (Ac) and Apis mellifera (Am) share an evolutionarily conserved set of QMP compounds: (E)-9-oxodec-2-enoic acid (9-ODA), (E)-9-hydroxydec-2-enoic acid (9-HDA), (E)-10-hydroxy-dec-2-enoic acid (10-HDA), 10-hydroxy-decanoic acid (10-HDAA), and methyl p–hydroxybenzoate (HOB) found at similar levels. However, evidence suggests there may be species-specific sensitivity differences to QMP compounds because Ac workers have higher levels of ovarian activation than Am workers. Using electroantennograms, we found species-specific sensitivity differences for a blend of the major QMP compounds and three individual compounds (9-HDA, 10-HDAA, and 10-HDA). As predicted, Am was more sensitive than Ac in all cases (1.3- to 2.7- fold higher responses). There were also species differences in worker retinue attraction to three compounds (9-HDA, HOB, and 10-HDA). In all significantly different cases, Am workers were 4.5- to 6.2-fold more strongly attracted than Ac workers were. Thus, Ac workers responded less strongly to QMP than Am workers, and 9-HDA and 10-HDA consistently elicited stronger antennal and retinue formation responses.

scholarly journals Authentication of Apis cerana Honey and Apis mellifera Honey Based on Major Royal Jelly Protein 2 Gene

Molecules ◽  
2019 ◽  
Vol 24 (2) ◽  
pp. 289 ◽  
Author(s):  
Yan-Zheng Zhang ◽  
Shuai Wang ◽  
Yi-Fan Chen ◽  
Yu-Qi Wu ◽  
Jing Tian ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Royal Jelly ◽  
Gene Selection ◽  
Apis Cerana ◽  
Melt Curve Analysis ◽  
Pcr Product ◽  
Authentication System ◽  
Rapid Discrimination ◽  
Species Specific ◽  
Royal Jelly Protein

In Asia, honey is mainly produced by Apis mellifera and Apis cerana. However, the price of A. cerana honey is usually much higher than A. mellifera honey. Seeing considerable profits, some dishonest companies and beekeepers mislabel A. mellifera honey as A. cerana honey or incorporate A. mellifera honey into A. cerana honey. In the present study, we developed methods to discriminate A. cerana honey from A. mellifera honey based on the MRJP2 (major royal jelly protein 2) gene. Two pairs of species-specific primers were designed. The amplification products of A. cerana and A. mellifera were 212 and 560 bp, respectively. As little as one percent incorporation of A. mellifera honey in the mixture can be detected by duplex PCR. Additionally, another method based on the melt curve analysis using the same primers was also developed, allowing a rapid discrimination of real-time PCR product of different species. Our study shows that the entomological authentication of honey samples can be identified by nuclear genes other than mitochondrial genes and this extends the possibility of gene selection in identification. The authentication system we proposed could be a useful tool for discriminating A. cerana honey from A. mellifera honey.

Occurrence of 9-oxodec-trans-2-enoic acid in queens of Apis dorsata, Apis cerana, and Apis mellifera

1970 ◽  
Vol 16 (7) ◽  
pp. 1437-1441 ◽  
Author(s):  
D.A. Shearer ◽  
R. Boch ◽  
R.A. Morse ◽  
F.M. Laigo
Keyword(s):  
Apis Mellifera ◽  
Apis Cerana ◽  
Enoic Acid ◽  
Apis Dorsata

scholarly journals Comparative Foraging Behavior of Apis Cerana F. and Apis Mellifera L. in Rapeseed under Cage Condition in Chitwan, Nepal

2014 ◽  
Vol 2 (4) ◽  
pp. 483-487
Author(s):  
Rameshwor Pudasaini ◽  
Resham Bahadur Thapa
Keyword(s):  
Apis Mellifera ◽  
Foraging Behavior ◽  
Apis Cerana ◽  
Lower Number ◽  
Pollen Collection ◽  
Cage Condition

An experiment was conducted to determine the foraging behavior of Apis mellifera L. and Apis cerana F. in rapeseed under cage condition in Chitwan, Nepal during 2012-2013. This experiment showed that Apis cerana F. foraged extra 42 minute per day as compared to Apis mellifera L. Apis cerana F. were more attracted to nectar, whereas Apis mellifera L. were more attracted to pollen collection throughout the day. The activities, in into hives and out from hives, for both species were recorded more at 2:00 pm and least at 8:00 am. The highest in-out were observed at 2:00 pm on both species as Apis mellifera L. 44.33 bees entered into hives and 49.66 bees went out of hives, whereas lower number of Apis cerana F. 43.66 bees entered into hives and 48.16 bees were out of hives. Apis mellifera L. collect 1.22:1 and 0.41:1 pollen nectar ratio at 10:00 am and 4:00 am whereas at same hours Apis cerana collect 1.16:1 and 0.30:1 pollen nectar ratio. Apis cerana F. foraged significantly higher number of rapeseed flowers and plants as compared to Apis mellifera L. under caged condition. It shows that Apis cerana F. was more efficient pollinator as compared to Apis mellifera L. under caged condition. DOI: http://dx.doi.org/10.3126/ijasbt.v2i4.11238Int J Appl Sci Biotechnol, Vol. 2(4): 483-487  

scholarly journals INFESTAÇÃO NATURAL DE Varroa jacobsoni EM Apis mellifera scutellata (HYMENOPTERA: APIDAE)

2000 ◽  
Vol 5 (1) ◽  
Author(s):  
A. PEGORARO ◽  
E. M. MARQUES ◽  
A.C. NETO ◽  
E.C. COSTA
Keyword(s):  
Apis Mellifera ◽  
Apis Cerana ◽  
Varroa Jacobsoni ◽  
Friedman Test ◽  
Apis Mellifera Scutellata ◽  
Biological Relationship ◽  
Natural Infestation

Varroa jacobsoni foi descrita em 1904 por Oldenans em Java em cria de Apis cerana. O nível de infestação com V. jacobsoni mede indiretamente o grau de tolerância da A. mellifera à V. jacobsoni. O estudo foi conduzido no Município de Mandirituba-PR. Os enxames foram capturados com caixa iscas. A percentagem de infestação V. jacobsoni foi resistrada mensalmente. Aplicando-se o teste de Friedman e usando-se o rank de cada colônia, separou-se os grupos de colônias homogêneas. A tendência sazonal foi demostrada com representação gráfica. O experimento foi delineado segundo blocos inteiramente casualizados. Em todas as amostras foi encontrado o ácaro V. jacobsoni. Diferenças significativas entre as colônias foram observadas. Na população de Apis mellifera scutellata existem três grupos homogêneos de colônias quanto ao nível de infestação com esse ácaro. O inverno é a época onde o grau de infestação com V. jacobsoni é mais elevado. Natural infestation of Apis mellifera scutellata (Hymenoptera; Apidae) by Varroa jacobsoni (Mesostigmata; Varroidae) Abstract Infestation by Varroa jacobsoni in an offspring of Apis cerana was first described as early as 1904 in Java. Since the level of infestation by V. jacobsoni may be an indirect procedure to measure the Apis mellifera scutellata tolerance degree towards it, the present research was carried out in order to evaluate such biological relationship between host and parasite and its implication in the Apis mellifera scutellata productivity. This study was carried out at Mandirituba, Paraná, Brazil. The swarms were captured with bait boxes. The percentage of V. jacobsoni infestation was established monthly. According to the Friedman test ant through the rank, homogeneous colonies were single out. The experiment has been delineated as entirely randomized blocks.

Evolutionary origin of the cell nucleus and its functional architecture

2010 ◽  
Vol 48 ◽  
pp. 1-24 ◽  
Author(s):  
Jan Postberg ◽  
Hans J. Lipps ◽  
Thomas Cremer
Keyword(s):  
Cell Nucleus ◽  
Interdisciplinary Research ◽  
Nuclear Organization ◽  
Nuclear Architecture ◽  
Evolutionary Origin ◽  
Cell Type ◽  
Research Strategies ◽  
Evolutionarily Conserved ◽  
Species Specific ◽  
The Impact

Understanding the evolutionary origin of the nucleus and its compartmentalized architecture provides a huge but, as expected, greatly rewarding challenge in the post-genomic era. We start this chapter with a survey of current hypotheses on the evolutionary origin of the cell nucleus. Thereafter, we provide an overview of evolutionarily conserved features of chromatin organization and arrangements, as well as topographical aspects of DNA replication and transcription, followed by a brief introduction of current models of nuclear architecture. In addition to features which may possibly apply to all eukaryotes, the evolutionary plasticity of higher-order nuclear organization is reflected by cell-type- and species-specific features, by the ability of nuclear architecture to adapt to specific environmental demands, as well as by the impact of aberrant nuclear organization on senescence and human disease. We conclude this chapter with a reflection on the necessity of interdisciplinary research strategies to map epigenomes in space and time.

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