A comparative study of cell attachment to self assembled monolayers and plasma polymers

1998 ◽  
Vol 8 (12) ◽  
pp. 2583-2584 ◽  
Author(s):  
Ros Daw ◽  
Ian M. Brook ◽  
A. Jane Devlin ◽  
Robert D. Short ◽  
Elaine Cooper ◽  
...  
1998 ◽  
Vol 544 ◽  
Author(s):  
D. B. Haddow ◽  
R. M. France ◽  
R. D. Short ◽  
S. Macneil ◽  
R. A. Dawson

AbstractHuman keratinocytes have been cultured on plasma co-polymers (PCPs), self assembled monolayers (SAMs), tissue culture poly(styrene) (TCPS) and collagen I. The degree of keratinocyte attachment was measured over 24 hours and cell proliferation and growth monitored over 7 days using optical microscopy and DNA concentrations. Cell attachment and proliferation and growth on the PCP surfaces were compared with 2 self assembled monolayer (SAM) systems. PCP surfaces containing carboxylic acid functionalities promoted keratinocyte attachment, with optimum attachment levels seen on surfaces containing less than 5% acid groups. The level of attachment on these surfaces was comparable to that seen on collagen I, a preferred substratum for the culturing of keratinocytes. After several days in culture the cells were well attached and proliferative. Keratinocytes attached well to acidterminated SAMs but attached poorly to methyl-terminated SAMs.


2006 ◽  
Vol 22 (2) ◽  
pp. 233-240 ◽  
Author(s):  
Warakorn Limbut ◽  
Proespichaya Kanatharana ◽  
Bo Mattiasson ◽  
Punnee Asawatreratanakul ◽  
Panote Thavarungkul

2003 ◽  
pp. 2231-2236 ◽  
Author(s):  
Izaura Cirino Nogueira Diógenes ◽  
Jackson Rodrigues de Sousa ◽  
Idalina Maria Moreira de Carvalho ◽  
Márcia Laudelina Arruda Temperini ◽  
Auro Atsushi Tanaka ◽  
...  

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