The metabonomics of aging and development in the rat: an investigation into the effect of age on the profile of endogenous metabolites in the urine of male rats using 1H NMR and HPLC-TOF MS

2005 ◽  
Vol 1 (2) ◽  
pp. 166 ◽  
Author(s):  
R. E. Williams ◽  
E. M. Lenz ◽  
J. S. Lowden ◽  
M. Rantalainen ◽  
I. D. Wilson
Keyword(s):  
1H Nmr ◽  
Male Rats ◽  
Endogenous Metabolites ◽  
Effect Of Age ◽  
Tof Ms

Effect of age on alveolar bone turnover adjacent to maxillary molar roots in male rats: A histomorphometric study

2007 ◽  
Vol 52 (1) ◽  
pp. 44-50 ◽  
Author(s):  
Yasuko Misawa ◽  
Toru Kageyama ◽  
Keita Moriyama ◽  
Saburo Kurihara ◽  
Hiroshi Yagasaki ◽  
...  
Keyword(s):  
Bone Turnover ◽  
Alveolar Bone ◽  
Male Rats ◽  
Histomorphometric Study ◽  
Maxillary Molar ◽  
Effect Of Age

Case study: monitoring of Glc4 tetrasaccharide in the urine of Pompe patients, use of MALDI-TOF MS, and 1H NMR

2018 ◽  
Vol 73 (3) ◽  
pp. 701-711 ◽  
Author(s):  
Zuzana Pakanová ◽  
Mária Matulová ◽  
Iveta Uhliariková ◽  
Darina Behúlová ◽  
Anna Šalingová ◽  
...  
Keyword(s):  
1H Nmr ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Study Monitoring ◽  
Tof Ms

scholarly journals Age and tissue specific differences in the development of acute insulin resistance following injury

2009 ◽  
Vol 203 (3) ◽  
pp. 365-374 ◽  
Author(s):  
Lidong Zhai ◽  
Joseph L Messina
Keyword(s):  
Insulin Resistance ◽  
Skeletal Muscle ◽  
Insulin Signaling ◽  
Intracellular Signaling ◽  
Male Rats ◽  
Hepatic Insulin Resistance ◽  
Surgical Trauma ◽  
Induce Insulin Resistance ◽  
Old Rats ◽  
Effect Of Age

Injuries, hemorrhage, sepsis, burn, and critical illnesses all induce insulin resistance, and insulin resistance is strongly associated with advancing age. However, the effect of age on injury induced insulin resistance is not well studied. We performed surgical trauma in male rats of three different ages (3-, 6-, and 10-weeks old). Rats were either hemorrhaged to a mean arterial pressure of 35–40 mmHg and subsequently maintained at that pressure for up to 90 min, or maintained without hemorrhage as controls. Results indicate that insulin-induced intracellular signaling was diminished in liver and skeletal muscle of 6- and 10-week old rats following trauma and hemorrhage. In even younger rats, immediately post-weaning (∼3 weeks of age), insulin signaling was lost in liver, but not in skeletal muscle. Glucocorticoids can play a role in the chronic development of insulin resistance. Our results demonstrate that corticosterone levels were increased in 6- and 10-week old animals following hemorrhage, but little change was measured in 3-week old animals. Blockade of glucocorticoid synthesis prevented the development of insulin resistance in skeletal muscle, but not in liver of 6- and 10-week old rats. Moreover, skeletal muscle glucocorticoid receptor levels increased dramatically between 3 and 6 weeks of age. These results indicate that trauma and hemorrhage-induced hepatic insulin resistance occurs at all ages tested. However, there is no development of insulin resistance following trauma and hemorrhage in skeletal muscle of post-weaning rats. In skeletal muscle of 6- and 10-week old rats, inhibition of glucocorticoid levels prevents the development of insulin resistance.

scholarly journals Integration of 1H NMR and UPLC-Q-TOF/MS for a Comprehensive Urinary Metabonomics Study on a Rat Model of Depression Induced by Chronic Unpredictable Mild Stress

PLoS ONE ◽  
2013 ◽  
Vol 8 (5) ◽  
pp. e63624 ◽  
Author(s):  
Hong-mei Jia ◽  
Yu-fei Feng ◽  
Yue-tao Liu ◽  
Xing Chang ◽  
Lin Chen ◽  
...  
Keyword(s):  
1H Nmr ◽  
Rat Model ◽  
Mild Stress ◽  
Chronic Unpredictable Mild Stress ◽  
Tof Ms

Dynamic analysis of the endogenous metabolites in depressed patients treated with TCM formula Xiaoyaosan using urinary 1H NMR-based metabolomics

2014 ◽  
Vol 158 ◽  
pp. 1-10 ◽  
Author(s):  
Jun-sheng Tian ◽  
Guo-jiang Peng ◽  
Xiao-xia Gao ◽  
Yu-zhi Zhou ◽  
Jie Xing ◽  
...  
Keyword(s):  
Dynamic Analysis ◽  
1H Nmr ◽  
Depressed Patients ◽  
Endogenous Metabolites

Comprehensive Non-Targeted Analysis of Contaminated Groundwater of a Former Ammunition Destruction Site using 1H-NMR and HPLC-SPE-NMR/TOF-MS

2009 ◽  
Vol 43 (18) ◽  
pp. 7055-7061 ◽  
Author(s):  
Markus Godejohann ◽  
Lea Heintz ◽  
Cristina Daolio ◽  
Jean-Daniel Berset ◽  
Daniel Muff
Keyword(s):  
1H Nmr ◽  
Contaminated Groundwater ◽  
Targeted Analysis ◽  
Tof Ms

Spontaneous running activity in male rats: effect of age

1985 ◽  
Vol 58 (5) ◽  
pp. 1553-1557 ◽  
Author(s):  
C. E. Mondon ◽  
C. B. Dolkas ◽  
C. Sims ◽  
G. M. Reaven
Keyword(s):  
Maximum Intensity ◽  
Male Rats ◽  
High Achievers ◽  
Maximal Rate ◽  
Running Records ◽  
Progressive Loss ◽  
Running Activity ◽  
Intensity Of Exercise ◽  
Effect Of Age

Alterations in the intensity and pattern of spontaneous running activity as rats increase in age from 7 wk to 1 yr was studied in male rats placed in exercise wheel cages. Daily running records were obtained on 27 rats for periods up to 12 mo, and 24-h activity recordings were made of selected runners to study the variation in activity during the day. The data indicate that for rats running over 2,940 revolutions (or 2 miles/day), the maximum intensity of running attained can be divided into a group of high achievers (approximately 8 miles/day) and moderate achievers (averaging 4.5 miles/day). For both groups, spontaneous running activity reached maximal rates after 4–5 wk. This maximal rate was sustained for 7–8 wk, then fell to levels approximately 60% of maximum for 4–5 mo, and then fell again to levels approximately 25% of maximum from 8 to 12 mo of age. The hourly pattern of running activity during the day was defined in rats of increasing age, who averaged 13,280, 6,662, 3,874, and 1,755 rev/day, corresponding to 9.0, 4.5, 2.6, and 1.2 miles/day, respectively. The overall patterns at each level indicated that the major running period occurred between 6:00 P.M. and 6:00 A.M., the greater activity of younger rats was paralleled by faster speeds and longer duration at each hour of the day, and the peak running activity for each group generally occurred between 7:00 and 9:00 P.M. In summary, there is a progressive loss in speed and duration of spontaneous running activity as male rats increase in age, with intensity of exercise falling below 2 miles/day after 7–8 mo of age.

scholarly journals Effects of the Chiral Fungicides Metalaxyl and Metalaxyl-M on the Earthworm Eisenia fetida as Determined by 1H-NMR-Based Untargeted Metabolomics

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1293 ◽  
Author(s):  
Renke Zhang ◽  
Zhiqiang Zhou
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Citric Acid ◽  
Magnetic Resonance ◽  
1H Nmr ◽  
Eisenia Fetida ◽  
Tricarboxylic Acid ◽  
Untargeted Metabolomics ◽  
1H Nuclear Magnetic Resonance ◽  
Endogenous Metabolites ◽  
Arginine Aspartate

Although metalaxyl and metalaxyl-M are widely used fungicides, very little is known about their subacute and enantiospecific effects on the earthworm metabolome. In this study, Eisenia fetida were exposed to metalaxyl and metalaxyl-M at three concentrations (0.5, 5 and 50 mg/kg) for seven days. 1H nuclear magnetic resonance (1H-NMR)-based untargeted metabolomics showed that metalaxyl and metalaxyl-M exposure disturbed earthworms’ metabolism at all three concentrations. Endogenous metabolites, such as succinate, arginine, aspartate, urea, asparagine, alanine, trimethylamine, taurine, cysteine, serine, threonine, histidine, lysine, glucose, choline, carnitine, citric acid, alpha-ketoisovaleric acid, fumaric acid and so on, were significantly changed. These results indicate that metalaxyl and metalaxyl-M produce different, enantiospecific disturbances in the earthworm metabolism, particularly in the tricarboxylic acid (TCA) and urea cycles. The application of untargeted metabolomics thus provides more information for evaluating the toxic risks of metalaxyl and metalaxyl-M.

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