Interaction of reducible polypeptide gene delivery vectors with supported lipid bilayers: pore formation and structure–function relationships

Soft Matter ◽  
2010 ◽  
Vol 6 (11) ◽  
pp. 2517 ◽  
Author(s):  
Mahmoud Soliman ◽  
Rujikan Nasanit ◽  
Stephanie Allen ◽  
Martyn C. Davies ◽  
Simon S. Briggs ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Structure Function ◽  
Lipid Bilayers ◽  
Pore Formation ◽  
Supported Lipid Bilayers ◽  
Gene Delivery Vectors

scholarly journals Silica nanoparticle supported lipid bilayers for gene delivery

2009 ◽  
pp. 5100 ◽  
Author(s):  
Juewen Liu ◽  
Alison Stace-Naughton ◽  
C. Jeffrey Brinker
Keyword(s):  
Gene Delivery ◽  
Lipid Bilayers ◽  
Silica Nanoparticle ◽  
Supported Lipid Bilayers

Structure–function relationships of gene delivery vectors in a limited polycation library

2005 ◽  
Vol 103 (1) ◽  
pp. 273-283 ◽  
Author(s):  
David J. Chen ◽  
Brian S. Majors ◽  
Alexander Zelikin ◽  
David Putnam
Keyword(s):  
Gene Delivery ◽  
Structure Function ◽  
Gene Delivery Vectors

scholarly journals Hypersonic poration of supported lipid bilayers

2019 ◽  
Vol 3 (5) ◽  
pp. 782-790 ◽  
Author(s):  
Yao Lu ◽  
Jurriaan Huskens ◽  
Wei Pang ◽  
Xuexin Duan
Keyword(s):  
Gene Delivery ◽  
Lipid Bilayers ◽  
Biomedical Applications ◽  
Membrane Disruption ◽  
Supported Lipid Bilayers ◽  
New Type ◽  
Disruption Method ◽  
Drug And Gene Delivery

Hypersound (ultrasound of gigahertz (GHz) frequency) has been recently introduced as a new type of membrane-disruption method for cells, vesicles and supported lipid bilayers (SLBs), with the potential to improve the efficiency of drug and gene delivery for biomedical applications.

Influence of Brain Gangliosides on the Formation and Properties of Supported Lipid Bilayers for Binding Kinetics Studies

2018 ◽  
Author(s):  
Luke Jordan ◽  
Nathan Wittenberg
Keyword(s):  
Lipid Bilayers ◽  
Binding Kinetics ◽  
Supported Lipid Bilayers ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Supported Lipid Bilayer ◽  
Head Groups ◽  
Lipid Diffusion ◽  
Kinetics Of ◽  
Brain Gangliosides

This is a comprehensive study of the effects of the four major brain gangliosides (GM1, GD1b, GD1a, and GT1b) on the adsorption and rupture of phospholipid vesicles on SiO2 surfaces for the formation of supported lipid bilayer (SLB) membranes. Using quartz crystal microbalance with dissipation monitoring (QCM-D) we show that gangliosides GD1a and GT1b significantly slow the SLB formation process, whereas GM1 and GD1b have smaller effects. This is likely due to the net ganglioside charge as well as the positions of acidic sugar groups on ganglioside glycan head groups. Data is included that shows calcium can accelerate the formation of ganglioside-rich SLBs. Using fluorescence recovery after photobleaching (FRAP) we also show that the presence of gangliosides significantly reduces lipid diffusion coefficients in SLBs in a concentration-dependent manner. Finally, using QCM-D and GD1a-rich SLB membranes we measure the binding kinetics of an anti-GD1a antibody that has similarities to a monoclonal antibody that is a hallmark of a variant of Guillain-Barre syndrome.

Recent advances in the analysis full/empty capsid ratio and genome integrity of adeno-associated virus (AAV) gene delivery vectors

2020 ◽  
Vol 20 ◽  
Author(s):  
L. Hajba ◽  
A. Guttman
Keyword(s):  
Gene Delivery ◽  
High Efficiency ◽  
Analytical Techniques ◽  
Viral Gene ◽  
Adeno Associated Virus ◽  
Gene Delivery Vectors ◽  
Empty Capsid ◽  
Purification Methods ◽  
Viral Gene Delivery

: Adeno-associated virus (AAV) is one of the most promising viral gene delivery vectors with long-term gene expression and disease correction featuring high efficiency and excellent safety in human clinical trials. During the production of AAV vectors,there are several quality control (QC)parameters that should be rigorously monitored to comply with clini-cal safety and efficacy. This review gives a short summary of the most frequently used AVV production and purification methods,focusing on the analytical techniques applied to determine the full/empty capsid ratio and the integrity of the encapsidated therapeutic DNA of the products.

scholarly journals Formation of Supported Lipid Bilayers Derived from Vesicles of Various Compositional Complexity on Conducting Polymer/Silica Substrates

Langmuir ◽  
2021 ◽  
Author(s):  
Hanna Ulmefors ◽  
Josefin Nissa ◽  
Hudson Pace ◽  
Olov Wahlsten ◽  
Anders Gunnarsson ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Conducting Polymer ◽  
Supported Lipid Bilayers ◽  
Silica Substrates

scholarly journals Measuring microtubule binding kinetics of membrane-bound kinesin motors using supported lipid bilayers

2021 ◽  
Vol 2 (3) ◽  
pp. 100691
Author(s):  
Rui Jiang ◽  
William O. Hancock
Keyword(s):  
Lipid Bilayers ◽  
Binding Kinetics ◽  
Supported Lipid Bilayers ◽  
Microtubule Binding ◽  
Membrane Bound ◽  
Kinetics Of

scholarly journals Delivery of Ricin Toxin A-Chain by Peptide-Targeted Mesoporous Silica Nanoparticle-Supported Lipid Bilayers

2012 ◽  
Vol 1 (3) ◽  
pp. 348-353 ◽  
Author(s):  
Katharine Epler ◽  
David Padilla ◽  
Genevieve Phillips ◽  
Peter Crowder ◽  
Robert Castillo ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Lipid Bilayers ◽  
Silica Nanoparticle ◽  
Supported Lipid Bilayers ◽  
Toxin A ◽  
Mesoporous Silica Nanoparticle ◽  
Ricin Toxin ◽  
A Chain

Effects of Ethanol and n-Butanol on the Fluidity of Supported Lipid Bilayers

2021 ◽  
pp. 105091
Author(s):  
Masroor Hossain ◽  
G.J. Blanchard
Keyword(s):  
Lipid Bilayers ◽  
Supported Lipid Bilayers
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