Interaction of reducible polypeptide gene delivery vectors with supported lipid bilayers: pore formation and structure–function relationships

Soft Matter ◽  
2010 ◽  
Vol 6 (11) ◽  
pp. 2517 ◽  
Author(s):  
Mahmoud Soliman ◽  
Rujikan Nasanit ◽  
Stephanie Allen ◽  
Martyn C. Davies ◽  
Simon S. Briggs ◽  
...  
2009 ◽  
pp. 5100 ◽  
Author(s):  
Juewen Liu ◽  
Alison Stace-Naughton ◽  
C. Jeffrey Brinker

2005 ◽  
Vol 103 (1) ◽  
pp. 273-283 ◽  
Author(s):  
David J. Chen ◽  
Brian S. Majors ◽  
Alexander Zelikin ◽  
David Putnam

2019 ◽  
Vol 3 (5) ◽  
pp. 782-790 ◽  
Author(s):  
Yao Lu ◽  
Jurriaan Huskens ◽  
Wei Pang ◽  
Xuexin Duan

Hypersound (ultrasound of gigahertz (GHz) frequency) has been recently introduced as a new type of membrane-disruption method for cells, vesicles and supported lipid bilayers (SLBs), with the potential to improve the efficiency of drug and gene delivery for biomedical applications.


2018 ◽  
Author(s):  
Luke Jordan ◽  
Nathan Wittenberg

This is a comprehensive study of the effects of the four major brain gangliosides (GM1, GD1b, GD1a, and GT1b) on the adsorption and rupture of phospholipid vesicles on SiO2 surfaces for the formation of supported lipid bilayer (SLB) membranes. Using quartz crystal microbalance with dissipation monitoring (QCM-D) we show that gangliosides GD1a and GT1b significantly slow the SLB formation process, whereas GM1 and GD1b have smaller effects. This is likely due to the net ganglioside charge as well as the positions of acidic sugar groups on ganglioside glycan head groups. Data is included that shows calcium can accelerate the formation of ganglioside-rich SLBs. Using fluorescence recovery after photobleaching (FRAP) we also show that the presence of gangliosides significantly reduces lipid diffusion coefficients in SLBs in a concentration-dependent manner. Finally, using QCM-D and GD1a-rich SLB membranes we measure the binding kinetics of an anti-GD1a antibody that has similarities to a monoclonal antibody that is a hallmark of a variant of Guillain-Barre syndrome.


2020 ◽  
Vol 20 ◽  
Author(s):  
L. Hajba ◽  
A. Guttman

: Adeno-associated virus (AAV) is one of the most promising viral gene delivery vectors with long-term gene expression and disease correction featuring high efficiency and excellent safety in human clinical trials. During the production of AAV vectors,there are several quality control (QC)parameters that should be rigorously monitored to comply with clini-cal safety and efficacy. This review gives a short summary of the most frequently used AVV production and purification methods,focusing on the analytical techniques applied to determine the full/empty capsid ratio and the integrity of the encapsidated therapeutic DNA of the products.


Langmuir ◽  
2021 ◽  
Author(s):  
Hanna Ulmefors ◽  
Josefin Nissa ◽  
Hudson Pace ◽  
Olov Wahlsten ◽  
Anders Gunnarsson ◽  
...  

2012 ◽  
Vol 1 (3) ◽  
pp. 348-353 ◽  
Author(s):  
Katharine Epler ◽  
David Padilla ◽  
Genevieve Phillips ◽  
Peter Crowder ◽  
Robert Castillo ◽  
...  

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